A Rapid Validated Uni-Dimensional Double Development Hptlc-Densitometry Method For Simultaneous Estimation Of Metformin Hydrochloride, Gliclazide And Pioglitazone Hydrochloride

Objective: To develop and validate a uni-dimensional double development high-performance thin layer chromatography (UDDD-HPTLC) for estimation of anti-diabetic medicine compromising of metformin (MET) gliclazide (GLZ) and pioglitazone hydrochloride (PIO). Methods: The chromatographic separation of these drugs was carried out on precoated TLC plates silica gel 60F254by two mobile phases consisting of Ammonium Sulphate: Methanol: Acetonitrile: Water (4:3:2:1) for MET and PIO and Toluene: Ethyl Acetate: Formic Acid (6:4:0.5) for GLZ respectively for ideal separation and good resolution. The densitometric detection and quantification were carried out at 237 nm for MET and 200 nm for GLZ and PIO. The validation parameters were strictly followed as per the ICH guidelines. Results: The linearity range was obtained at 3000-8000ng/spot, 360-960 ng/spot, 90-240 ng/spot for MET, GLZ and PIO with r2value>0.999. The other parameters such as precision, reproducibility, robustness were efficiently obtained within the limits. The proposed method was successfully applied for simultaneous determination of MET, GLZ and PIO in the commercial formulation. Conclusion: In simultaneous estimation, the different polarity of drugs makes it more cumbersome to develop and validate any chromatographic method. In the present study, a uni-dimensional double development high-performance thin layer chromatography (UDDD-HPTLC) for estimation of these drugs have been developed and validated to resolve the estimation problem. It is an effortless and speedy method which was developed and validated using ICH guidelines. The developed and validated method using ICH guidelines is effortless and speedy technique.

Investigation of the stability of adriamycin liposome by TLC / 重庆医科大学学报

Objective:To investigate the usability of TLC as a evaluating method for adriamycin liposome stability by separating its composition and degradation products with TLC and improve this method. Methods:The plates were developed twice with four development systems,visualized by 4-methoxybenzaldehyde. Results:It was better to separate EPC,cholesterol and their degradation products by using chloroform-methanol-water-ammonia(65∶35∶2.5∶2.5) as the mobile phase. Conclusion:TLC could be used for the quality control of the adriamycin liposome due to its simple operation and high reproducibility.