RESUMO
@#Nitric oxide (NO) as a signal transduction species and/or effector molecule plays an extremely important role in the cardiovascular, nervous, and immune systems. Insufficient production or abnormal signal transduction of NO is closely associated with onset and progression of many diseases. Studies on direct or indirect regulation of NO levels in vivo and related metabolic pathways have become a hot spot in biomedical and pharmaceutical fields. This review briefly introduces the generation of NO in vivo and its physiological and pathological functions in cardio-cerebrovascular system, with focus on the authors'' research on NO-donating cardio-cerebrovascular and related drugs, aiming to provide some reference for future development of these drugs.
RESUMO
Objective To define the relationship between genes expression of 5 immune effector molecules and acute rejection of renal allografts. Methods The magnitude of intragraft Fas, Fas ligand (FasL), granzyme B(GB) , perforin(P) and T cell intracellular antigen-1 (TIA-1) mRNA were quantified by competitive PCR in 42 samples. Results were expressed as the ratio of picograms of target gene to picograms of ?-actin, and were compared with the histological diagnosis based upon Banff criteria. Results Of all 42 tissues, the transcripts were detectable in 41 for Fas, 11 for FasL, 19 for GB, 33 for P and 27 for TIA-1. Intragraft expression of FasL, GB, P and TIA-1 but not Fas was significantly elevated in acute rejection group as compared with chronic rejection group ( P