Nephrotoxicity screening of main active components in Zuojin Pills / 中国药理学通报

Aim To study the nephrotoxicity effects of the main monomers in Zuojin Pills. Methods CCK-8 and high-content toxicity screening were used to preliminarily screen the main alkaloids in Zuojin Pills that may cause renal cell damage. Further, by confirmation of cell morphology, release rate of lactate dehydrogenase and cytochrome C, and expression of apoptosis-related proteins, the alkaloids causing cell damage were preliminarily identified, providing in vitro toxicological evidence for the compatibility of components of traditional Chinese medicine and compatibility attenuation. Results Preliminary screening using CCK-8 method and high-content technology showed that evodiamine (EVO) could significantly reduce cell number, increase cell membrane permeability, and reduce mitochondrial membrane potential. In addition, cell morphology, apoptosis and cytochrome C expression were consistent with the results of high-content screening. Western blot experiments indicate that EVO could induce apoptosis and cause renal cell damage. Conclusions EVO can obviously cause renal cell damage, and may induce apoptosis by affecting mitochondria, cytochrome C and cell membrane permeability.

Identification of the regulatory effect of evodiamine on functional regulation gene expressing in murine dendritic cell by microarrays analysis / 中国免疫学杂志

Objective:cDNA microarrary technique was employed to detect the effects of evodiamine on functional regulation of gene expressing in murine marrow-derived dendritic cells.Methods:Immature dendritic cells (iDC) derived from bone marrow of BALB/c mice were induced by GM-CSF for ten days in vitro and received corresponding treatment for 24 h.Then the cell were randomized into the control groups (Ⅰ),evoamine group (Ⅱ),LPS group (Ⅲ) and evodiamine+LPS group (Ⅳ).After 24 h’s treatment, total RNA of the cells was collected,and cDNA microarrary (GEArray S Series Mouse Dendritic & Antigen Presenting Cell Gene Array:MM-604) was used to screen the functionalg correlation genes of dendritic cells.Results:There were seven genes up-regulated and two genes down-regulated two-folds above in Ⅱ/Ⅰ;thirty four genes up-regulated and twelve genes dwon-regulated two-folds above in Ⅲ/Ⅰ;forty six genes up-regulated and seven genes down-regulated two-folds above in Ⅳ/Ⅱ;twenty four genes up-regulated and two genes down-regulated two-folds above in Ⅳ/Ⅲ.The function of these genes involve the secret of the cytokines and the expression of their receptors,antigen uptake,antigen presentation,cell surface receptors and signal transduction.Conclusion:The effects of evodiamine on dendritic cell involve in multi-regulation and expression of genes,these genes contribute to the functions,differentiation and maturation of the dendritic cells.The work provides a potentiality to seek the target of medicine.