RESUMO
Extracellular matrix (ECM) is a major component of the cellular microenvironment.ECM not only provides physical support for cells, hut also participates in various biological processes.In recent years, hundreds of cancer-related ECM (C-ECM) genes have been identified, and some of them can he used as biomarkers.However, rich information about C-ECM genes is scattered among thousands of publications, and there is no systematic summary of their role in tumorigenesis.Here, we present the CECMAtlas (http://biokb.ncpsb.org.cn/CECMAtlas/), a database which collects 225 C-ECM genes and the information of biological processes that they participate in through literature mining and manual curation.This database will contribute to the study of the mechanism of tumorigenesis and possible clinical application.
RESUMO
BACKGROUND: Diabetes mellitus is a heterogeneous group of disorders characterized by high serum glucose levels and by disturbances of carbohydrate and lipid metabolism. There are many cutaneous signs of this common endocrinopathy, such as nercobiosis lipoidica diabeticorum, diabetic bullosis, shin spot, diabetic pruritus, etc. OBJECTIVE: In this study, we investigated whether extracellular matrix gene expression in cultured skin fibroblast is influenced by insulin and Insulin-like growth factor-I(IGF-I). METHOD: Total RNA was isolated from insulin or IGF-I treated human skin fibroblasts. The Northern blot and slot-blot hybridization were then conducted. RESULTS: The mRNA levels of pro α1(I) collagen, pro α1(I11) collagen, fibronectin in insulin and IGF-I treated normal skin fibroblasts increased compared with untreated normal skin fibroblasts. CONCLUSION: Our results show that insulin and IGF-I stimulate collagen formation in normal skin fibroblast at physiological concentrations. Therefore, these demonstrate that insulin can modulate the expression of extracellular matrix gene.