FASTR: A novel data format for concomitant representation of RNA sequence and secondary structure information.

Given the importance of RNA secondary structures in defining their biological role, it would be convenient for researchers seeking RNA data if both sequence and structural information pertaining to RNA molecules are made available together. Current nucleotide data repositories archive only RNA sequence data. Furthermore, storage formats which can frugally represent RNA sequence as well as structure data in a single file, are currently unavailable. This article proposes a novel storage format, ‘FASTR’, for concomitant representation of RNA sequence and structure. The storage efficiency of the proposed FASTR format has been evaluated using RNA data from various microorganisms. Results indicate that the size of FASTR formatted files (containing both RNA sequence as well as structure information) are equivalent to that of FASTA-format files, which contain only RNA sequence information. RNA secondary structure is typically represented using a combination of a string of nucleotide characters along with the corresponding dot-bracket notation indicating structural attributes. ‘FASTR’ – the novel storage format proposed in the present study enables a frugal representation of both RNA sequence and structural information in the form of a single string. In spite of having a relatively smaller storage footprint, the resultant ‘fastr’ string(s) retain all sequence as well as secondary structural information that could be stored using a dot-bracket notation. An implementation of the ‘FASTR’ methodology is available for download at http://metagenomics.atc.tcs.com/compression/fastr.

Way of DIB Derived from DICOM Medical Image File / 中国医学物理学杂志

Objective: According to clinical investigation, analysis on the storage format of DICOM3.0 medical images files and displaying them is the basis of medical image processing. It is very important for medical imaging technology. Methods: Firstly, with the DICOM standard, the format of the medical images is studied, mostly the format of the information header and data element. Then the DIB display of the DICOM images in Windows platform is put forward, using linear window-transformation. Results: It is successfully realized the display of CT、MR images. With the manual window-transformation, the displaying would be better. Conclusion: As the results show, the method studied in the paper can preferably realize the display of the DICOM medical images, meeting certain clinical needs, and laying a foundation for the following study.