RESUMO
Objective Observe the fine distribution and morphologic feature of the intramural lymphatics in rats' rectum and to provide the morphological evidence for studying the mechanism of lymphous metastasis of rectum carcinoma.Methods The intramural lymphatics of rectum in rats were studied by light and electron microscopy to obtain some data regarding their fine distribution.Results The intramural capillary lymphatics of rats' rectum were observed in deep lamina propria of tunica membrane and the dermal papilla layer of anal pectin. The intramural capillary lymphatics and lymphatics were both observed in tela submucosa, reticular layer, hypodermis and intermuscular and serosa. The capillary lymphatics in rectums' wall have the similar ultrastructure feature to normal ones. Three kinds of endothelial cells junctions are overlapping-junctions, embedding-junctions and end-to-end-type junctions. About 21% of cell-junctions are opening types. There are normal organelles and many vesicals in the endothelial of capillary lymphatics. Conclusion The distribution of lymphatics in every layer of dentate line upwards are similar to other digestive tubes'. The distribution of lymphatics in anal pecten of rats' rectum are similar to cutis. The results show that big molecule substance and tissue hydrolysate are transporting mainly by opening-junction of endothelial cells and the vesicle system.
RESUMO
Since the conventional dyes and puncture injection give no satisfactory effect in observing the pulmonary deep lymphatics, the author is inspired to introduce two new kinds of mixture, one is AgNO_3 with India ink, one is Riger's solution with India ink. Injections of the new mixtures directly into the artery, and the following findings are obtained. 1.With the lymphatic vessel related to the pulmonary artery as the main current, the pulmonary deep lymphatic vessels extend themselves to somewhere near the meta-arteriole while the lymphatic capillaries can be found around the inter-alveolar septa close to the terminal arterioles. 2.The pre-lymphovascular fluid pathway is carefully observed, which is described by Professor T. Kihara as of sponge like reticular fibres, around the lymphatic capillary blind sacs under the external base lamina propria of bronchioles. It is convinced that the lymphatic capillary, which is not yet found under the respiratory bronchiole membrane, starts where the final terminal bronchiole branches. 3.The fact that the valve is absent in the lymphatic vessel under the external membrane of both the bronchiole and the arteriole of related to the terminal bronchiolus shows that the lymphatic vessel farther off is capillary. 4.The tube of the pulmonary lymphatic capillary appears, under the microscope, irregular in shape where its endothelial cells, which are thin all over except for the part at the nuclei, have no pericytes and are lacking in the laminae or just have scattered ones. The cells are loosely joined in various ways and in some places they are separated by anchoring filaments.