Formación de la cultura metrológica desde las potencialidades del deporte y la Educación Física

Este artículo trata la formación de la cultura metrológica, en los estudiantes de la Educación Preuniversitaria, desde las potencialidades del deporte y las clases de Educación Física, como uno de los elementos que debe ser priorizado en el proceso de enseñanza-aprendizaje de la Física y como parte de la preparación de los estudiantes para la vida. El objetivo de este trabajo consistió en diseñar un sistema de tareas docentes integradoras que permita aprovechar las potencialidades del deporte, en general, y las clases de Educación Física, en particular para la formación de la cultura metrológica en los estudiantes de décimo grado. Para el desarrollo de la investigación se utilizaron los métodos: analítico-sintético, inductivo-deductivo, modelación, observación, análisis documental, encuesta, entrevista y estadística descriptiva que permitieron procesar la información referente al tema objeto de investigación; así como determinar las causas de las insuficiencias que se presentan en el proceso de enseñanza-aprendizaje de la Física, para la formación de la cultura metrológica de los estudiantes de décimo grado. El sistema de tareas docentes integradoras propuesto se caracterizó por armonizar núcleos conceptuales básicos para el tratamiento a los contenidos metrológicos como son la estimación y medición de magnitudes y la conversión de unidades de medida; además de aprovechar las potencialidades del deporte y las clases de Educación Física.

Este artigo trata da formação da cultura metrológica em alunos da Educação Pré-Universitária, a partir das potencialidades das aulas de esportes e de Educação Física, como um dos elementos que devem ser priorizados no processo de ensino-aprendizagem de Física e como parte da preparação dos alunos para a vida. O objetivo deste trabalho foi conceber um sistema de tarefas didáticas integradoras que permita aproveitar as potencialidades do esporte, em geral, e das aulas de Educação Física, em particular, para a formação da cultura metrológica em alunos do décimo ano. Para desenvolver a pesquisa foram utilizados os seguintes métodos: analítico-sintético, indutivo-dedutivo, modelagem, observação, análise documental, levantamento, entrevista e estatística descritiva que permitiram o tratamento das informações referentes ao tema sob investigação; bem como determinar as causas das insuficiências que surgem no processo de ensino-aprendizagem de Física, para a formação da cultura metrológica dos alunos do décimo ano. O sistema de tarefas de ensino integrativo proposto caracterizou-se por harmonizar núcleos conceituais básicos para o tratamento de conteúdos metrológicos como a estimativa e medição de grandezas e a conversão de unidades de medida; além de aproveitar o potencial das aulas de esportes e Educação Física.

This article deals with the formation of metrological culture in Pre-University Education students, from the potential of sports and Physical Education classes, as one of the elements that must be prioritized in the teaching-learning process of Physics and as part of preparing students for life. The objective of this work was to design a system of integrative teaching tasks that allows taking advantage of the potential of sport, in general, and Physical Education classes, in particular, for the formation of metrological culture in tenth grade students. To develop the research, the following methods were used: analytical-synthetic, inductive-deductive, modeling, observation, documentary analysis, survey, interview and descriptive statistics that allowed the processing of information regarding the topic under investigation; as well as determine the causes of the insufficiencies that arise in the teaching-learning process of Physics, for the formation of the metrological culture of tenth grade students. The proposed system of integrative teaching tasks was characterized by harmonizing basic conceptual cores for the treatment of metrological content such as the estimation and measurement of magnitudes and the conversion of measurement units; in addition to taking advantage of potential of the sports and Physical Education classes.

Stimulation of mouse hematopoietic stem cells by angiogenin and DNA preparations

Immature hematopoietic progenitors are a constant source for renewal of hemocyte populations and the basic component of the tissue and cell repair apparatus. A unique property of these cells of internalizing extracellular double-stranded DNA has been previously shown. The leukostimulatory effect demonstrated in our pioneering studies was considered to be due to the feature of this cell. In the present research, we have analyzed the effects of DNA genome reconstructor preparation (DNAgr), DNAmix, and human recombinant angiogenin on both hematopoietic stem cells and multipotent progenitors. Treatment with bone marrow cells of experimental mice with these preparations stimulates colony formation by hematopoietic stem cells and proliferation of multipotent descendants. The main lineage responsible for this is the granulocyte-macrophage hematopoietic lineage. Using fluorescent microscopy as well as FACS assay, co-localization of primitive c-Kit- and Sca-1-positive progenitors and the TAMRA-labeled double-stranded DNA has been shown. Human recombinant angiogenin was used as a reference agent. Cells with specific markers were quantified in intact bone marrow and colonies grown in the presence of inducers. Quantitative analysis revealed that a total of 14,000 fragment copies of 500 bp, which is 0.2% of the haploid genome, can be delivered into early progenitors. Extracellular double-stranded DNA fragments stimulated the colony formation in early hematopoietic progenitors from the bone marrow, which assumed their effect on cells in G0. The observed number of Sca1+/c-Kit+ cells in colonies testifies to the possibility of both symmetrical and asymmetrical division of the initial hematopoietic stem cell and its progeny.

Dos nuevos registros para la flora agrostológica de Perú / Two new records for the agrostological flora of Peru

Resumen Se reporta por primera vez la presencia de Piptochaetium bicolor (Vahl) É. Desv. y Trichachne californica var. villosissima (Henrard) Wipff & Shaw para Perú, basada en colecciones realizadas en el ecosistema de formación de lomas costeras del departamento de Arequipa. Se presentan descripciones, fotografías, ilustraciones y una clave taxonómica para cada taxón.

Abstract The presence of Piptochaetium bicolor (Vahl) É. Desv. and Trichachne californica var. villosissima (Henrard) Wipff & Shaw are recorded for the first time from Peru, based upon collections made in the coastal lomas formations ecosystem of the department of Arequipa. Descriptions, photographs, illustrations and a taxonomic key are presented for each taxon.

Changes in pulmonary pericytes and tube formation of pulmonary vascular endothelial cells in mouse models of broncho-pulmonary dysplasia / 中国组织工程研究

BACKGROUND:Pulmonary pericytes are located at the concavity where pulmonary vessels are interconnected,which is closely related to the formation and stability of pulmonary vascularization.However,there are few studies on how pulmonary pericytes affect the activity of pulmonary vascular endothelial cells in the pathogenesis of broncho-pulmonary dysplasia. OBJECTIVE:To analyze the relationship between the quantity of subgroups of pulmonary pericytes and endothelial cells in different stages of broncho-pulmonary dysplasia and to explore the effects of PDGFR-β+NG2+α-SMA+ pericytes on the early tube-forming activity of pulmonary vascular endothelial cells. METHODS:(1)Animal experiment:Twelve newborn C57BL/6 mice were randomly divided into normoxia and hyperoxia groups within 24 hours of birth,with six mice in each group.Mice in the hyperoxia group were exposed to an 85%O2 environment to build the mouse models of broncho-pulmonary dysplasia,while those in the normoxia group were fed in the same room air.The lung tissues of the mice in the two groups were taken at 7 and 14 days after birth.The pathological changes of the lung tissues were observed by hematoxylin-eosin staining.Three subgroups of pulmonary pericytes were measured by flow cytometry:PDGFR-β+NG2+α-SMA-,PDGFR-β+NG2+α-SMA+,and PDGFR-β+NG2-α-SMA+ cells.(2)Cellular experiment:Passage 3 PDGFR-β+NG2+α-SMA+ pericytes were co-cultured with mouse pulmonary vascular endothelial cells(experimental group)at a ratio of 1:4.Mouse pulmonary vascular endothelial cells cultured alone were used as controls.The tube-forming difference between two groups was analyzed after 15 hours of co-culture. RESULT AND CONCLUSION:(1)Animal experiment:Hematoxylin-eosin staining revealed that on day 7,the lung tissue of mice in the normoxia group had regular structure,obvious alveolar structure,and uniform size,while the number of alveoli in the lung tissue of mice in the hyperoxia group was less and the morphology of alveoli was irregular.On day 14,the alveoli of mice in the normoxia group gradually developed and matured,the alveolar structure gradually became regular and uniform in size,and the alveolar density gradually increased.The lung tissue structure of mice in the hyperoxia group was relatively disordered and the alveolar formation was delayed with the size gradually increasing and the alveolar structure being simplified.Flow cytometry results indicated that the number of PDGFR-β+NG2-α-SMA+ and PDGFR-β+NG2+α-SMA+ pericytes was increased in the hypoxia group compared with the normoxia group(P＜0.01),while the number of PDGFR-β+NG2+α-SMA-pericytes and pulmonary vascular endothelial cells was decreased(P＜0.01,P＜0.04).(2)Cellular experiment:In the control group,the pulmonary vascular endothelial cells arranged in cords and extended around,and lumen-like structures formed in some areas.In the experimental group,PDGFR-β+NG2+α-SMA+ pericytes and their pseudopodia were not observed,the irregular grid structure of pulmonary vascular endothelial cells was significantly less than that of the control group,and the endothelial cells mainly clustered in clumps.To conclude,α-SMA+ pericyte subgroups are predominant in mice with broncho-pulmonary dysplasia.PDGFR-β+NG2+α-SMA+ pericytes can directly inhibit the tube-forming activity of pulmonary vascular endothelial cells,which may be involved in the process of abnormal vascularization in broncho-pulmonary dysplasia.

Exosome-derived microRNA with bone and joint diseases:role and mechanism / 中国组织工程研究

BACKGROUND:Exosomes are vesicle-like structures secreted by cells into extracellular compartments in the form of cytosol,which contain a large amount of microRNAs with important intercellular communication roles.MicroRNAs in exosomes rely on exosome transport and are able to enter target cells to exert important biological regulatory effects.In common bone and joint diseases,abnormal or damaged bone metabolism releases a large number of exosomes,while some exosome-derived microRNAs also promote the progression of osteoarthritis.Therefore,exosome-derived microRNAs are closely related to the skeletal system and are important for the development as well as diagnosis and treatment of many osteoarticular diseases. OBJECTIVE:To review the research progress of exosome-derived microRNAs in bone metabolism and bone and joint diseases. METHODS:Using"exosomes,extracellular vesicle,microRNA,miRNA,bone,bone diseases,bone formation,bone regeneration,bone resorption,bone destruction"as Chinese and English search terms,articles were searched on CNKI,Metasys,and PubMed databases.Finally,86 articles were included for summarization. RESULTS AND CONCLUSION:Exosome-derived microRNAs can regulate bone metabolism by affecting bone formation and bone resorption,and are closely related to the development of bone and joint diseases such as fracture healing,osteoporosis,osteoarthritis,rheumatoid arthritis,osteonecrosis of the femoral head,and osteosarcoma.Exosome-derived microRNAs will be an effective means of diagnosis and treatment of certain bone and joint diseases in the future.However,the current research on exosome-derived microRNAs in osteoarthritic diseases is limited,and more explorations and researches are still needed to diagnose and treat osteoarthritic diseases using exosome-derived microRNAs.

Role of autophagy in orthodontic tooth movement / 中国组织工程研究

BACKGROUND:The application of orthodontic force triggers autophagy in the periodontal tissue via diverse signaling pathways,augmenting or attenuating the activity of relevant cell types such as periodontal ligament cells,osteocytes,osteoclasts,and osteoblasts,thus facilitating the process of periodontal remodeling. OBJECTIVE:To review the research progress in orthodontic force mediated autophagy in periodontal tissue and its impact on orthodontic tooth movement. METHODS:The PubMed,Web of Science,China Biology Medicine disc and CNKI were searched for literature published from 2010 to 2023 to summarize the progress in orthodontics-related autophagy.And 76 papers were finally included in the analysis and discussion. RESULTS AND CONCLUSION:Orthodontic force can trigger a series of biochemical signal changes through periodontal mechanical receptors and aseptic inflammation they cause,leading to autophagy in periodontal tissue.Subsequently,autophagy generates corresponding feedback through cascaded amplified signaling pathways such as Phosphoinositide 3-kinase/protein kinase B,Hippo,and mitogen-activated protein kinase pathways,promoting periodontal tissue remodeling and ultimately achieving tooth movement and stability.Orthodontic force-induced autophagy can differentially regulate bone resorption on the tooth pressure side and bone formation on the tension side.Related targets have good prospects in the clinical application of orthodontic treatment.Orthodontics and autophagy have complex mechanisms.However,existing research has only focused on exploring the role of autophagy in orthodontic tooth movement.Further exploration is needed to investigate the mutual regulatory effects between autophagy and orthodontic tooth movement,as well as the interactions between upstream mechanical receptors and signaling pathways involved in related pathways.

Research progress on inhibition of filtration area scarring after implantation of Ahmed glaucoma valve / 中华实验眼科杂志

Ahmed glaucoma drainage valve (AGV) implantation is one of the main methods for the treatment of refractory glaucoma with a higher success rate than conventional filtration surgery.However, as a foreign body, the AGV often causes hyperplasia of scar tissue in the filtration area, wrapping around the drainage plate, thereby inhibiting aqueous fluid outflow and causing the intraocular pressure to rise again, leading to surgical failure.Although multiple injections of anti-metabolic drugs during and after AGV implantation can inhibit postoperative scarring, multiple postoperative subconjunctival injections will not only cause discomfort to patients, but also lead to complications.Therefore, it is necessary to improve the AGV to avoid repeated injection of the drug, achieve slow local release of the drug, and reduce the foreign body reaction of AGV at the same time.Recently, the development of new materials, such as Ologen collagen, poly (2-hydroxyethyl methacrylate), poly lactic-co-glycolic acid and opal shale and new techniques provides new methods to inhibit the scarring of filtration area after AGV implantation.This article reviews the methods and progress of inhibition of scar formation in filtration area from the aspects of development of AGV drainage plate materials, construction of drug delivery system of AGV combined with new materials, and improvement of AGV drainage plate structure.

Infiltration of neutrophil extracellular traps promotes activation of ure-thral fibroblasts and scar formation after urethral trauma / 中国病理生理杂志

AIM:This study was performed to investigate the impact of neutrophil extracellular traps(NETs)on scar formation following urethral trauma.METHODS:(1)Clinical samples were derived from patients of Department of Urology,The First Affiliated Hospital of Fujian Medical University,from June 2021 to December 2022.Levels of NETs in the blood and urine were compared between patients with urethral trauma(n=20)and those without urethral trauma(controls,n=20).The relationship between NETs and scar formation was analyzed.(2)Urethral fibroblasts were isolated from urethral scar tissues,and neutrophils were induced to produce NETs in vitro.The urethral fibroblasts were treated with normal saline,0.5 mg/L NETs,or 1.5 mg/L NETs to investigate the effects of NETs on activation and collagen syn-thesis of urethral fibroblasts.Additionally,a rabbit model of urethral trauma was established and the animals were dioided into four groups to explore the therapeutic potential of deoxyribonuclease I(DNase I)in preventing urethral scar forma-tion:control,operation + transforming growth factor-β1(TGF-β1),operation + normal saline,and operation+DNase I.RESULTS:The level of NETs in urine increased after urethral trauma(P<0.05),but the level of NETs in blood did not change(P>0.05).In the animal models,the urethral scar became more severe as the level of NETs in the urine increased(P<0.05).At the cellular level,NETs promoted the viability,migration,and collagen synthesis of urethral fibroblasts(P<0.05)..Additionally,urethral injection of DNase I after trauma reduced the level of NETs and inhibited the formation of urethral scar tissue in the animal models(P<0.05).CONCLUSION:Infiltration of NETs promotes activation of urethral fibroblasts and scar formation after urethral trauma.

Silencing Nodal inhibits the biological behavior of retinal vascular endothelial cells under high glucose conditions / 中华眼底病杂志

Objective:To observe the effect of Nodal on the biological behavior of retinal vascular endothelial cells (RF/6A cells) in monkeys with high glucose.Methods:RF/6A cells were divided into normal group, mannitol group, high glucose group, high glucose combined with non-specific small interfering RNA treatment group (HG+NC group), high glucose combined with small interfering Nodal treatment group (HG+siNodal group). The transfection efficiency of siNodal was observed by real-time fluorescence quantitative PCR and western blot protein immunoblotting. The effect of Nodal on the proliferation of RF/6A cells was detected by thiazole blue colorimetry. The effect of Nodal on migration ability of RF/6A cells was detected by cell scratch assay. The effect of Nodal on the formation of RF/6A cell lumen was measured by Matrigel three-dimensional in vitro. The expression of extracellular signal phosphorylated regulated kinase 1/2 (pERK1/2) in RF/6A cells was detected by western blot protein immunoblotting. One-way analysis of variance was used to compare groups.Results:Compared with HG+NC group, Nodal protein ( F=33.469) and mRNA relative expression levels ( F=38.191) in HG+siNodal group were significantly decreased, cell proliferation was significantly decreased ( F=28.548), and cell migration ability was significantly decreased ( F=24.182). The number of cell lumen formation was significantly decreased ( F=52.643), and the differences were statistically significant ( P<0.05). Compared with HG+NC group, the relative expression of pERK1/2 protein in HG+siNodal group was significantly decreased, and the difference was statistically significant ( F=44.462, P<0.01). Conclusions:Silencing Nodal expression can inhibit proliferation, migration and tube formation of RF/6A cells induced by high glucose. It may act by inhibiting pERK1/2 expression.

Study on the Intervention Effect of β-sitosterol in the Postmenopausal Osteoporosis / 浙江中医药大学学报

[Objective]To explore the intervention effect of β-sitosterol,an effective monomer component of Eucommia ulmoides,on postmenopausal osteoporosis in mice and its potential mechanism.[Methods]Twenty-four female C57BL/6J mice of 12 weeks old were randomly divided into sham operation group,model group and β-sitosterol intervention group,with 8 mice in each group.The model of postmenopausal osteoporosis was established by bilateral ovariectomy.Mice in sham group and model group were given 0.9％sodium chloride solution and β-sitosterol intervention group was given β-sitosterol.Eight weeks after operation,all the mice were sacrificed and samples were collected.Micro-computed tomography(Micro-CT)was used to observe the protective effect of the β-sitosterol intervention on postmenopausal bone loss and trabecular bone breakage.Hematoxylin-eosin(HE)staining was used to analyze the bone histomorphology of the distal femur.The tartrate-resistant acid phosphatase(TRAP)staining and the alkaline phosphatase(ALP)staining were used to evaluate bone resorption and bone formation respectively.Then the expression level of β-catenin in each group was detected by immunohistochemistry.[Results]Micro-CT scanning showed that β-sitosterol could reduce bone loss and bone microstructure destruction in postmenopausal mice.HE staining also confirmed that β-sitosterol could effectively maintain the number and morphology of trabecular bone and inhibit abnormal fat accumulation caused by estrogen deficiency.TRAP staining also showed that β-sitosterol treatment did not affect osteoclast formation,while immunohistochemical results showed that β-sitosterol could significantly promote the expression of osteogenic marker ALP.Moreover,β-sitosterol can increase the expression of β-catenin in the distal femur to promote bone formation.[Conclusion]β-sitosterol treatment could enhance osteogenic differentiation and promote bone formation to relieve postmenopausal osteoporosis by upregulating β-catenin expression in vivo.

Mechanism of calcium oxalate kidney stone formation mediated by autophagy and endoplasmic reticulum stress pathway regulated by p38 MAPK pathway / 现代泌尿外科杂志

【Objective】 To explore the effects and mechanism of p38 mitogen activated protein kinase (MAPK) pathway on the formation of calcium oxalate (CaOx) kidney stones in rats,so as to provide new ideas for the treatment of kidney stones. 【Methods】 A total of 40 rats were divided into control, SB203580, CaOx and SB203580+CaOx groups, with 10 rats in each group.Intragastric administration of a mixture of 1% ethylene glycol and 1% ammonium chloride was given to the CaOx and SB203580+CaOx groups to construct CaOx models, while intragastric administration of drinking water was given to the control and SB203580 groups.After molding, SB203580 and SB203580+CaOx groups were injected with 5 mg/kg SB203580 peritoneally once a day for 14 days, while the control and CaOx groups were injected with equal volume of normal saline.The renal mass of rats was measured and the renal coefficient was calculated; the serum levels of blood urea nitrogen (BUN) and serum creatinine (SCr) were measured with an automated biochemical analyzer; the urinary levels of neutrophil gelatinase-associated lipid carrier protein (NGAL) and kidney injury molecule-1 (KIM-1) were determined with enzyme-linked immunosorbent assay (ELISA); the crystal deposition and tissue damage in renal tissues were observed with Von Kossa staining; the apoptosis of renal tubule cells was observed with TUNEL; the expressions of autophagy markers in kidney tissues were detected with immunohistochemical staining; the molecular expressions of autophagy-endoplasmic reticulum stress related pathways in renal tissues were determined with RT-qPCR and Western blot. 【Results】 Compared with the CaOx group, the SB203580+CaOx group had increased body mass after molding (P<0.05); decreased kidney mass, kidney coefficient, BUN, SCr, NGAL and KIM-1 levels (P<0.05); alleviated pathological damage of kidney tissues; significantly reduced black crystal; down-regulated proportion of positive TUNEL cells, positive expression area of LC3B and Beclin-1, mRNA expressions of LC3B, Beclin-1, CHOP and GRP78, protein ratio of LC3-Ⅱ/LC3-Ⅰ, and protein expressions of Beclin-1, CHOP and GRP78 (P<0.05); but up-regulated mRNA and protein expressions of p62 (P<0.05). 【Conclusion】 The p38 MAPK pathway is involved in the formation of CaOx kidney stones in rats.Inhibition of this pathway can reduce the formation of kidney stones, which may be related to the regulation of autophagy and endoplasmic reticulum stress.

Effect of α-Klotho on macrophage-vascular endothelial cell crosstalk in diabetic oxidative stress environment / 国际眼科杂志(Guoji Yanke Zazhi)

AIM:To investigate the effects of overexpressing α-Klotho(KL)in RAW264.7 cells stimulated by oxidative stress on the proliferation, migration, tube-formation and tight junction of human umbilical vein endothelial cells(HUVECs).METHODS:RAW264.7 cells were categorized into control, 4-hydroxynonenal(4HNE), and 4HNE+KL groups, with F4/80 expression assessed via immunofluorescence staining. Three groups of conditional media were prepared for HUVECs and culture divided into M&#x0026;#xF8;-NC, M&#x0026;#xF8;-4HNE, and M&#x0026;#xF8;-4HNE+KL groups. Cell proliferation was evaluated using CCK8 assay, while scratch test and Transwell assays were employed to measure cell migration. Additionally, tube-formation assay was conducted to assess cell tubule formation, and Western blot assay was utilized to detect the protein expression levels of Claudin 5, Occludin and ZO 1.RESULTS:The results of immunofluorescence staining showed that the fluorescence intensity of F4/80 of RAW264.7 cells in the 4HNE group was significantly enhanced compared with the control group, while that of F4/80 in the 4HNE+KL group was significantly decreased compared with the 4HNE group(all P&#x003C;0.05). The CCK8 assay results revealed a significant increase in the proliferation of HUVECs in the M&#x0026;#xF8;-4HNE group compared with the M&#x0026;#xF8;-NC group. Conversely, the proliferation of the M&#x0026;#xF8;-4HNE+KL group exhibited a significant decrease compared with that in the M&#x0026;#xF8;-4HNE group(all P&#x003C;0.01). The results of scratch test and Transwell assays demonstrated a significant increase in the migration of HUVECs in the M&#x0026;#xF8;-4HNE group compared with the M&#x0026;#xF8;-NC group, while the migration of the M&#x0026;#xF8;-4HNE+KL group exhibited a significant decrease compared with the M&#x0026;#xF8;-4HNE group(all P&#x003C;0.01). In the tube-formation assay, it was observed that the number of tubes formed by HUVECs in the M&#x0026;#xF8;-4HNE group was significantly increased compared with the M&#x0026;#xF8;-NC group, while that of tubes formed in the M&#x0026;#xF8;-4HNE+KL group was significantly decreased compared with the M&#x0026;#xF8;-4HNE group(all P&#x003C;0.01). Additionally, the Western blot results revealed a significant decrease in the relative expression levels of Claudin 5, Occludin, and ZO 1 in the M&#x0026;#xF8;-4HNE group compared with the M&#x0026;#xF8;-NC group. Conversely, in the M&#x0026;#xF8;-4HNE+KL group, there was a significant increase in the relative expression levels of Claudin 5, Occludin, and ZO 1 compared to the M&#x0026;#xF8;-4HNE group(all P&#x003C;0.01).CONCLUSIONS: KL inhibits the proliferation, migration, and tube-formation of HUVECs while enhancing the tight junction by changing the activation state of macrophages in the diabetic oxidative stress environment.

Curcumin plays an anti-osteoporosis role by inhibiting NF-κB signaling pathway to reduce oxidative stress damage to osteogenesis / 中国药理学通报

Aim To investigate the mechanism of curcumin inhibition of oxidative stress on osteogenic differentiation and its dose-dependent anti-osteoporosis effect. Methods Cellular oxidative stress models were used, different concentrations of curcumin were added to determinethebone formation markers, and the potential signaling pathways involvedwere detected. Meanwhile, the mouse model of osteoporosis ( ovariecto- mized, 0VX) was used to confirm its effect against osteoporosis. Results In vitro experiments found that low concentrations of curcumin (1-10 μmol · L

The lysine methyltransferase SMYD2 facilitates neointimal hyperplasia by regulating the HDAC3-SRF axis

Coronary restenosis is an important cause of poor long-term prognosis in patients with coronary heart disease. Here, we show that lysine methyltransferase SMYD2 expression in the nucleus is significantly elevated in serum- and PDGF-BB-induced vascular smooth muscle cells (VSMCs), and in tissues of carotid artery injury-induced neointimal hyperplasia. Smyd2 overexpression in VSMCs (Smyd2-vTg) facilitates, but treatment with its specific inhibitor LLY-507 or SMYD2 knockdown significantly inhibits VSMC phenotypic switching and carotid artery injury-induced neointima formation in mice. Transcriptome sequencing revealed that SMYD2 knockdown represses the expression of serum response factor (SRF) target genes and that SRF overexpression largely reverses the inhibitory effect of SMYD2 knockdown on VSMC proliferation. HDAC3 directly interacts with and deacetylates SRF, which enhances SRF transcriptional activity in VSMCs. Moreover, SMYD2 promotes HDAC3 expression via tri-methylation of H3K36 at its promoter. RGFP966, a specific inhibitor of HDAC3, not only counteracts the pro-proliferation effect of SMYD2 overexpression on VSMCs, but also inhibits carotid artery injury-induced neointima formation in mice. HDAC3 partially abolishes the inhibitory effect of SMYD2 knockdown on VSMC proliferation in a deacetylase activity-dependent manner. Our results reveal that the SMYD2-HDAC3-SRF axis constitutes a novel and critical epigenetic mechanism that regulates VSMC phenotypic switching and neointimal hyperplasia.

Live Animal Training in Surgical Education for Undergraduate Medical Students / 医学教育

A live animal training program is offered to medical students seeking more advanced surgical education. This program requires active participation in all aspects, from planning to implementation.　The program provides medical students with a valuable opportunity to develop their interest in surgical care and to acquire advanced surgical techniques through a step-by-step approach. Additionally, they have the chance to develop various types of non-technical skills by assuming different roles within a team. Instructors offer support for their learning.　Peer learning allows medical students to learn from and discuss with each other, enabling them to acquire a broad range of knowledge and skills. Simultaneously, students are expected to understand the importance of a team approach by participating in this program from the perspectives of various medical professions. Moreover, the program contributes to the formation of their professional identity.

Analysis on Pathogenesis and Treatment of Pulmonary Sarcoidosis Based on the Theory of"Yang Leading to Qi and Yin Leading to Formation" / 中国中医药信息杂志

Theory of"yang leading to qi and yin leading to formation",as the law of nature giving birth to life,has wide applicability and can be used to interpret the nature and pathogenesis characteristics of pulmonary sarcoidosis.Based on yin-yang theory,this article elaborated on the pathological essence of pulmonary sarcoidosis.Under the guidance of the theory of"yang leading to qi and yin leading to formation",the treatment strategy of discerning the degree of yang qi deficiency and stagnation when forming pulmonary sarcoidosis,replenishing and unblocking the body's yang qi,and simultaneously soothing the liver and lungs,clearing collaterals,detoxifying,and dispersing masses to dissipate yin and coagulation,has certain guiding significance for clinical diagnosis and treatment of pulmonary sarcoidosis.

Effect of N-acetyl cysteine, rifampicin, and ozone on biofilm formation in pan-resistant Klebsiella pneumoniae: an experimental study

ABSTRACT BACKGROUND: To the best of our knowledge, this is the first study to evaluate the effectiveness of specific concentrations of antibiofilm agents, such as N-acetyl cysteine (NAC), rifampicin, and ozone, for the treatment of pan-resistant Klebsiella pneumoniae (PRKp). OBJECTIVES: We evaluated the effectiveness of antibiofilm agents, such as NAC, rifampicin, and ozone, on biofilm formation in PRKp at 2, 6, 24, and 72 h. DESIGN AND SETTING: This single-center experimental study was conducted on June 15, 2017, and July 15, 2018, at Istanbul Faculty of Medicine, Istanbul University, Turkey. METHODS: Biofilm formation and the efficacy of these agents on the biofilm layer were demonstrated using colony counting and laser-screened confocal microscopy. RESULTS: NAC at a final concentration of 2 μg/mL was administered to bacteria that formed biofilms (24 h), and no significant decrease was detected in the bacterial counts of all isolates (all P > 0.05). Rifampicin with a final concentration of 0.1 μg/mL was administered to bacteria that formed biofilm (24 h), and no significant decrease was detected in bacterial count (all P > 0.05). Notably, ozonated water of even 4.78 mg/L concentration for 72 h decreased the bacterial count by ≥ 2 log10. CONCLUSION: Different approaches are needed for treating PRKp isolates. We demonstrate that PRKp isolates can be successfully treated with higher concentrations of ozone.

Fundamentos do processo de trabalho em terapia ocupacional: uma abordagem analítica a partir do diálogo entre Terapia Ocupacional Social e Saúde Coletiva Latino-Americana / Labor process foundations of occupational therapy: an analytical approach based on the dialogue between Social Occupational Therapy and Latin American Collective Health / Fundamentos del proceso de trabajo de la terapia ocupacional: una aproximación analítica basada en el dialogo entre Terapia Social Ocupacional y Salud Colectiva Latinoamericana

Resumo Introdução Este artigo apresenta uma abordagem analítica da prática da terapia ocupacional baseada no materialismo histórico dialético, compreendendo que a prática profissional da terapia ocupacional é considerada trabalho no sistema capitalista, podendo ser analisada à luz da Teoria do Processo de Trabalho. Objetivo Propor uma abordagem analítica com o intuito de apoiar o desenvolvimento de práticas de terapia ocupacional emancipatórias, baseadas no diálogo entre a Terapia Ocupacional Social e a Saúde Coletiva Latino-Americana. Método Com base na pesquisa-ação emancipatória, foram realizadas dez oficinas com dez terapeutas ocupacionais visando à produção coletiva de conhecimento. Resultados A atividade humana foi identificada como objeto do processo de trabalho da terapia ocupacional, definida pela categoria trabalho em sua dimensão ontológica. A participação radical foi proposta como o produto intencionado deste processo, sendo entendida como a participação na luta por transformar elementos da determinação social das condições coletivas de trabalho e vida. Os sujeitos desse processo são os terapeutas ocupacionais e os indivíduos, grupos e/ou comunidades acompanhados, todos entendidos como seres sociais em parceria para a transformação social. São identificadas quatro ferramentas teórico-metodológicas: análise emancipatória de atividades humanas, proposição emancipatória de atividades humanas, operacionalização da participação radical e intervenções sobre o tecido social. Conclusão A terapia ocupacional como prática social oferece uma contribuição específica para o desenvolvimento de uma práxis revolucionária coletiva que, voltada para a realização de uma utopia concreta, poderá gerar os meios para produzir uma nova sociedade.

Abstract Introduction This paper presents an analytical approach to occupational therapy practice grounded in historical dialectical materialism. It understands occupational therapy professional practice as work within a capitalist system, and thus subject to analysis through the labor process theory. Objective To propose an analytical approach to occupational therapy practice aimed at supporting the development of an emancipatory practice framework based on the dialogue between Social Occupational Therapy and Latin American Collective Health. Method Utilizing emancipatory action research methodologies, this study conducted 10 workshops with 10 occupational therapists aimed at collective knowledge production. Results Human activity is identified as the object of occupational therapy working process, defined by the category work in its ontological dimension. Radical participation is set as the intended product of this process, which entails engaging in efforts to transform the social determination of collective living conditions. The individuals in this process include occupational therapists and the followed-up individuals, groups, and/or communities, all viewed as social beings collaborating toward social transformation. Four theoretical-methodological tools are identified: emancipatory analysis of human activities, emancipatory proposition of human activities, operationalization of radical participation, and interventions in the social fabric. Conclusion Occupational therapy, as a form of social practice, offers a specific contribution to the development of a collective revolutionary praxis. This praxis, aimed at realizing a concrete utopia, seeks to generate the means to create a new society.

Resumen Introducción Este artículo presenta una aproximación analítica a la práctica de la terapia ocupacional basada en el materialismo histórico dialéctico. Entiende la práctica profesional de terapia ocupacional como trabajo bajo el capitalismo y, como tal, puede ser analizada bajo la teoría del proceso de trabajo. Objetivo Proponer un enfoque analítico de la práctica de la terapia ocupacional con la intención de apoyar el desarrollo posterior de un marco para la práctica de la terapia ocupacional emancipadora basada en el dialogo entre la Terapia Ocupacional Social y la Salud Colectiva Latinoamericana. Método A partir de una investigación acción emancipadora, se realizaron 10 talleres con diez terapeutas ocupacionales con el objetivo de la producción colectiva de conocimiento. Resultados La actividad humana es identificada como objeto del proceso de trabajo de la terapia ocupacional, definida por la categoría trabajo en su dimensión ontológica. La participación radical se establece como el producto previsto del proceso, es decir, la participación en la lucha por los elementos transformadores de la determinación social de las condiciones de vida colectivas. Los sujetos del proceso de trabajo son los terapeutas ocupacionales y los individuos, grupos y/o comunidades acompañados, todos entendidos como seres sociales en colaboración para la transformación social. Se identifican cuatro herramientas teórico-metodológicas: análisis emancipatorio de las actividades humanas, propuesta emancipadora de las actividades humanas, operacionalización de la participación radical e intervenciones en el tejido social. Conclusión La terapia ocupacional como práctica social ofrece una contribución específica al desarrollo de una praxis revolucionaria colectiva que, dirigida a realizar una utopía concreta, puede generar los medios para producir una nueva sociedad.

A Percepção de Graduandos sobre a Psicologia Organizacional e do Trabalho / The Perception of Graduates on Work and Organizational Psychology / La Percepción de Graduandos sobre la Psicología Organizacional y Laboral

Resumo: A Psicologia Organizacional e do Trabalho (POT) é permeada por uma série de julgamentos negativos e estigmas, principalmente acerca de seu papel intermediador de relações de exploração sofridas pelo trabalhador. Investigar as percepções que graduandos têm sobre a área pode trazer acesso ao que há de central entre as diversas formas de pensar o tema. Este trabalho buscou analisar as percepções de 100 graduandos de duas instituições de ensino superior (IES) do Centro-Oeste Paulista, uma pública e outra privada, acerca da importância da atuação do psicólogo em POT e o interesse desses discentes em atuarem futuramente na área, estabelecendo uma comparação entre os resultados com base nas IES e entre primeiro e quinto ano da graduação. Os graduandos responderam a dois questionários estruturados com perguntas abertas e fechadas. Os resultados apontaram uma maior valorização da área de POT na IES privada e no quinto ano. A maioria dos discentes do primeiro ano de graduação apresentou pouco conhecimento sobre a POT. Os estudantes da IES privada apresentaram maior interesse em atuar na área do que os discentes da IES pública. Quando perguntados se houve uma mudança de opinião após a realização das disciplinas, 49 alunos do quinto ano responderam positivamente e apenas um respondeu negativamente. As respostas dissertativas se dividiram entre visões positivas, neutras e negativas sobre a POT. Os resultados permitiram avaliação das percepções da POT entre os graduandos, bem como das transformações ocorridas pelo impacto das experiências acadêmicas vivenciadas.

Abstract: Organizational and Work Psychology (OWP) is permeated by a series of negative judgments and stigmas, mainly about its role as an intermediary of relations exploiting workers. Investigating the perceptions undergraduates have about it can bring access to what is central among the different ways of thinking about the subject. This study sought to analyze the perceptions of 100 undergraduates from two higher education institutions (HEIs), one public and private in Midwestern São Paulo about the importance of psychologists' role in OWP and their interest in working in the area in the future, comparing the results per HEI and between the first and fifth year of graduation. Graduates answered two structured questionnaires with open and closed questions. Results pointed to a greater appreciation of OWP in the private HEI and in the fifth year. Most first-year undergraduate students had little knowledge about OWP. Students from the private HEI took greater interest in working in the area than students from the public HEI. When asked if taking the courses changed their opinions, 49 students responded positively and only one negatively. Participants' responses were divided into positive, neutra,l and negative views about OWP. Results evaluated the perceptions on OWP in undergraduates the changes due to the impact of academic experiences.

Resumen: La Psicología Organizacional y Laboral (POT) está permeada por una serie de juicios negativos y estigmas, principalmente sobre su papel como intermediaria de las relaciones de explotación laboral. Investigar las percepciones que los estudiantes tienen sobre este campo puede brindar acceso a lo que es central entre las diferentes formas de pensar sobre el tema. Este estudio buscó analizar las percepciones de 100 graduandos de dos instituciones de educación superior (IES), una pública y otra privada, del medio oeste de São Paulo, acerca de la importancia del rol del psicólogo en POT y del interés por actuar en el área, estableciendo una comparación entre los resultados por tipo de IES y período del curso (el primer y quinto año de grado). Los graduandos respondieron a dos cuestionarios estructurados con preguntas abiertas y cerradas. Los resultados apuntan a una mayor valoración de POT en las IES privadas y en el quinto año. La mayoría de los estudiantes de primer año tenían poco conocimiento sobre POT. Los estudiantes de la IES privada estaban más interesados en trabajar en el área que los estudiantes de la IES pública. Cuando se les preguntó si hubo un cambio de opinión luego de cursar las asignaturas, 49 estudiantes del quinto año respondieron positivamente y solo uno respondió negativamente. Las respuestas del ensayo se dividieron en puntos de vista positivos, neutrales y negativos sobre POT. Los resultados permitieron evaluar las percepciones de POT entre los graduandos, así como evaluar los cambios ocurridos por el impacto de las experiencias académicas.

O nó da terra: a renda da terra como base para análise das questões urbana e agrária / The land knot: land income as basis for analyzing urban and agrarian issues

Resumo: O objetivo deste artigo é discutir a renda da terra como base para análise das questões urbana e agrária. Para isso, realizamos um estudo temático cuja análise seguiu o método materialista histórico-dialético. Debruçamo-nos sobre os estudos desenvolvidos por Marx e Engels, além de elaborarmos uma pesquisa bibliográfica complementar. Buscou-se desvelar o laço que unifica a essência de duas expressões da questão social: a propriedade privada da terra e o processo de sua valorização a partir do trabalho incorporado à terra.

Abstract: This article aims to discuss land rent as a basis for analyzing urban and agrarian issues. To this end, we carried out a thematic study whose analysis followed the historical-dialectic materialist method. We focused on the studies developed by Marx, Engels, as well as complementary bibliographical research. We sought to unveil the bond that unifies the essence of these expressions of the "social issue": private ownership of land and the process of its valorization based on the work incorporated into the land.