Metal-organic Framework Immobilized Enzyme and Its Application in Screening of Enzyme Inhibitors of Traditional Chinese Medicine：A Review / 中国实验方剂学杂志

Enzymes are widely used in chemical and pharmaceutical industries because of their advantages of high efficiency and specificity. However， the shortcomings of the free enzymes， such as poor stability and difficulty in recycling， limit their application. Therefore， the immobilization and application of enzymes have become one of the research hotspots. The selection of the immobilization carriers is a critical step in the process of enzyme immobilization. Metal-organic frameworks（MOFs）， a kind of porous materials， are formed by the coordination of metal ions or metal clusters with organic ligands. As an emerging immobilization carrier， its advantages such as high porosity， strong stability， and surface modifiability make it ideal for immobilized enzyme carriers. By immobilizing the free enzyme on MOFs， the above mentioned deficiencies of the free enzymes can be effectively solved， which greatly broaden the applicable condition. Ligand fishing is a method to find receptor-specific ligands from complex components， which has the advantages of high efficiency， simple sample pretreatment and high specificity. The MOF-enzyme complex formed by enzyme immobilization can act as a "fishing rod" for ligand fishing， which can screen out the targets from the complex system of components. The complex chemical composition and various active ingredients of traditional Chinese medicine（TCM） make the ligand fishing technology to play a big role in the screening of enzyme inhibitors from TCM. And the screened enzyme inhibitors are expected to be further developed into the lead compounds with good efficacy and low adverse effects， so the immobilized enzymes of MOFs have a wide application in the screening of active ingredients from TCM. Based on this， this paper summarized the methods of immobilized enzymes of MOFs in recent years， analyzed the characteristics， advantages and disadvantages of each method， and summarized the laws of preparation conditions and mechanisms. Meanwhile， the application and future development of immobilized enzymes of MOFs in the field of enzyme inhibitor screening from TCM were also summarized and prospected， with a view to providing a reference for the development of natural ingredients and the modernization of TCM.

Construction of Chinese Herbal Medicine Traceability System Based on Influencing Factors of Chinese Herbal Medicine Quality / 中国实验方剂学杂志

As a Chinese saying goes， "good Chinese medicinal material makes good medicine"， the quality of Chinese herbal medicines is related to the development prospect of Chinese medicine industry in China. With the rapid development of new technologies such as traceability methods and monitoring instruments， it is imperative to integrate and innovate traditional Chinese herbal medicines with new-generation information technology in view of the quality problems existing in the current production and circulation of Chinese herbal medicines， and it is of great significance for the construction of traceability system to ensure the quality and safety of Chinese herbal medicines and to promote the industry of Chinese herbal medicines to move towards high-quality development. This paper reviews the development history of the traceability system of Chinese herbal medicines in China， takes the influencing factors of the quality of Chinese herbal medicines as the entry point， and proposes that the construction of the traceability system should satisfy the traceability requirements of the characteristics of Chinese herbal medicines and their traditional medication experience. By analyzing the influencing factors of the quality of Chinese herbal medicines， it is pointed out that focusing on the influencing factors to build a traceability system is of great significance for targeting the problematic links at a later stage and exploring the interrelationship between environmental factors and the quality of Chinese herbal medicines. Based on the previous explorations， the author summarizes the system framework， functional modules and practical applications of the traceability system of Chinese herbal medicines， and looks forward to the development of a traceability system with risk early warning function and expert decision-making function in its functional development. Finally， based on the factors affecting the quality of Chinese herbal medicines， the author puts forward several thoughts on construction of the traceability system， and makes an in-depth analysis and puts forward a solution for the current situation that a unified， standardized and universal traceability system has not yet been built， with a view to providing ideas and references for the construction of traceability system of Chinese herbal medicines.

The Screen Time Ecological Model (STEM): The development of a conceptual and practice model on screen time for children

@#It is undeniable that technology has become part of modern society that extends to children's grasp. However, the amount of screen time exposure in children and its effects are increasingly becoming a significant concern for families, educators, and child-related health professionals, such as occupational therapists. This study aims to help child-related health professionals understand the factors contributing to screen time and describe children's screen time levels to enable proper assessment and intervention planning. The Screen Time Ecological Model (STEM) follows the SocioEcological Model in describing influencing factors to screen time behavior, the Ecology of Human Performance in conceptualizing intervention approaches, and the Developmental Frame of Reference in describing the age-appropriate screen time recommendation children based on the American Academy of Pediatrics Media Use Guidelines for Families. The optimal goal of STEM is to promote the just-right screen time use within the pediatric population.

THE EVOLUTION OF CYBERSECURITY: ASSESSING THE EFFECTIVENESS OF LEGAL FRAMEWORKS IN THE FACE OF EMERGING TECHNOLOGIES AND TRANSNATIONAL CYBER ATTACKS

This research article aims to examine the impact of emerging technologies on cybersecurity and evaluate the effectiveness of legal frameworks in addressing transnational cyber-attacks. With the rapid advancement of technology, cyber threats have become more sophisticated and interconnected, requiring robust legal measures to combat them. With the emergence of new technologies, the threat landscape for cyber security has also evolved. The continuous development of new technologies like (A.I) artificial intelligence, (I.O.T) Internet of Things & cloud computing has led to a rapid increase in cyber-attacks and has made it even more challenging for organizations to protect their assets. This study utilizes a mixed-methods approach, incorporating both qualitative and quantitative research methods. The qualitative aspect involves an analysis of existing literature, case studies, and legal frameworks, while the quantitative component includes surveys and interviews with cybersecurity professionals and legal experts. By integrating these methods, this research aims to provide valuable insights into the effectiveness of current legal measures in mitigating transnational cyber-attacks and propose strategies to enhance cybersecurity in the face of emerging technologies.

Quais as consequências da aprovação do novo arcabouço fiscal para o SUS? / What are the implicationsof the adoptionof the new fiscal framework for the Brazilian Unified Health System? / ¿Cuáles son los efectosde la aprobación del nuevo marco fiscal para el Sistema Único de Salud del Brasil?

Quais as consequências da aprovação do novo arcabouço fiscal para o Sistema Único de Saúde (SUS)? Esta é a pergunta que norteia este editorial que reflete, com base em estudos econômicos e normativas federais, a necessidade de aprovação de um arcabouço fiscal que dialogue com um projeto de sistema universal de saúde, não desconsiderando o contexto histórico de desfinanciamento estrutural do SUS frente aos seus princípios, objetivos e diretrizes sistematizados pela nossa Constituição Federal.

What are the implications of the approval of the new fiscal framework for the Brazilian Unified Health System (SUS)? This question is the focus this editorial, which, based on economic studies and federal regulations, reflects the need to approve a fiscal framework consistent with the project of auniversal health system, not ignoring the historical context of structural underfunding of the SUS given its principles, objectives and guidelines systematized in Brazilian Federal Constitution.

¿Cuáles son los efectos de la aprobación del nuevo marco fiscal para el Sistema Único de Salud (SUS) del Brasil? Esa es la pregunta que guía este editorial, que refleja, con base en estudios económicos y normas federales, la necesidad de aprobar un marco fiscal que dialogue con un proyecto de sistema universal de salud, sin olvidar el contexto histórico de desfinanciamiento estructural del SUS frente de sus principios, objetivos y lineamientos sistematizados por la Constitución Federal de Brasil.

Writing A Research Framework For An Evaluation Of The Effectiveness Of Sustainable Tourism Policy Implementation In Cambodia: A Case Of Angkor World Heritage Area

This article aims at writing a research framework for exploring sustainable cultural heritage tourism policy implementation at Angkor (World Heritage Area). There are six policy initiatives within Angkor's Tourism Management Plan (2012-2020) to guide sustainability of tourism development. The year 2020 was the end of implementing these policy initiatives, but there were not any documents reporting the effectiveness and success of their implementations; therefore, this study will show the framework for investigating the implementation of policy initiatives within this timeframe. There are three main objectives in this framework. First, it is necessary to identify the outcomes of policy implementation judged by stakeholders. Second, it is important to outline and understand the barriers that hinder the successful implementation of the policy. Third, there is a need to create a framework for how to successfully implement sustainable tourism policies in the development of more sustainable tourism in the future.

Systematic construction of undergraduate curriculum for rehabilitation based on WHO rehabilitation competency framework / 中国康复理论与实践

ObjectiveTo systematically develop the courses of rehabilitation based on World Health Organization rehabilitation competency framework (RCF). MethodsThe theories and methods of RCF were used to analyze competency characteristics of rehabilitation workers and educational objectives to construct the curriculum contents in rehabilitation. ResultsThe competency of the rehabilitation workers included practice, professionalism, learning and development, management and leadership, research, values and beliefs in the specific rehabilitation situations. The objectives of rehabilitation medicine education needed to correspond to the competencies of the rehabilitation workers and set up knowledge units that could develop competency in terms of behaviour, activity tasks and proficiency. The curriculum systems of rehabilitation with RCF included the fields of rehabilitation physiotherapy, rehabilitation occupational therapy, hearing and speech rehabilitation, and prosthetic and orthopaedic engineering, etc. ConclusionThe teaching objectives corresponding to the competency characteristics of rehabilitation workers need to be translated into competency-based education. The curriculum contents in rehabilitation needs to be systematically constructed based on RCF from the areas of rehabilitation physiotherapy, rehabilitation occupational therapy, hearing and speech rehabilitation, and prosthetic and orthopaedic engineering.

Developing curriculum of speciality of education and rehabilitation using WHO rehabilitation competency framework / 中国康复理论与实践

ObjectiveTo construct rehabilitation competency framework (RCF) of the speciality of education and rehabilitation (SER-RCF) based on World Health Organization RCF, to innovate the curriculum system. MethodsThe competency for undergraduate level professionals in the speciality of education and rehabilitation were analyzed to develop curriculum using RCF theory and methodology. ResultsTalent training was a kind of competency-based and outcome-based education. SER-RCF integrated the competencies and activities in the areas of professionalism, practice, learning and development, management and leadership, and research required for professional work in education and rehabilitation around core values and beliefs. It also designed the training objectives of the speciality of education and rehabilitation: to be ethical and correct, to be physically and mentally healthy, to have a solid foundation, to be sustainable, to be co-operative and enjoyable, and to be reflective and enquiring. SER-RCF played a central role in developing the curriculum system of rehabilitation, education and psychology, and practice. ConclusionReshaping the undergraduate level curriculum system of education and rehabilitation based on RCF is not only the implementation of the construction concept of new medicine and new liberal arts, but also can effectively connect with the international standard of rehabilitation higher education.

Application of WHO rehabilitation competency framework in development of rehabilitation science education system and curriculum / 中国康复理论与实践

ObjectiveTo systematically analyze the application of World Health Organization rehabilitation competency framework (RCF) and its supporting guidelines in education system of rehabilitation science and curriculum development. MethodsBased on the conceptual framework of RCF and its supporting guidelines, the application of its areas and the modes were analyzed to construct the education system of rehabilitation science with reference to the framework of competency-based clinical medical education. ResultsThe education system of rehabilitation science was developed based on RCF. The competences for specific scenarios in rehabilitation were defined and investigated. A structured system of vocational qualification accreditation and occupational competency standards was constructed, as well as core education system and rehabilitation curricula in rehabilitation science, which helped to train competent rehabilitation workers. ConclusionThe construction of rehabilitation subspecialties, its education system and the development of a curriculum content system based on the RCF is an important way for the development of modern rehabilitation education. RCF theoretical framework and supporting tools provide a standardized and unified method and way for China's rehabilitation science higher education system and professional certification, career access and professional standards for rehabilitation personnel. The quality of rehabilitation science education and the professional development of rehabilitation personnel will also be improved in future.

Construction of basic rehabilitation training course for family doctor teams serving for people with disabilities based on WHO rehabilitation competency framework / 中国康复理论与实践

ObjectiveTo develop basic training courses for family doctor teams for people with disabilities. MethodsUtilizing the methods and theories of the World Health Organization (WHO) rehabilitation competency framework (RCF), and referring to the WHO universal health coverage global competency framework, the rehabilitation competency characteristics of family doctor teams for people with disabilities in community settings were analyzed, and a basic training course system for these teams based on the RCF was developed. Results and ConclusionBased on RCF, a competency framework for family doctor teams serving people with disabilities has been constructed. The objectives, content and training course system for basic rehabilitation training has been established.

Survey of rehabilitation competency of Chinese rehabilitation physicians using WHO rehabilitation competency framework / 中国康复理论与实践

ObjectiveTo investigate rehabilitation competence among trainers and trainees whom participated in the training program entitled National Training Program of Shortage Rehabilitation Physicians (NTPSRP) using World Health Organization rehabilitation competency framework (RCF). MethodsBased on RCF, a questionnaire was developed and administrated through network using Questionnaire Star. All the trainers and trainees who participated in NTPSRP were investigated in September, 2023. ResultsA total of 911 subjects were collected from 27 provinces, including 426 trainers (teachers) and 485 trainees (students). The average age of the teachers was older, with more years of work experience and professional experience in rehabilitation medicine. The degree and professional title of the teachers was also higher. The overall internal consistency reliability of the questionnaire was good (Cronbach's α = 0.988), and all the internal consistency reliability in five fields was good (Cronbach's α > 0.9). The five dimensional structure of RCF in the results was consistent with the prior model established by RCF theory. The self-ratings of all physicians in the five fields were higher than the median level, and was higher in the teachers than in the students [(3.42±0.68) vs. (2.73±0.80), P < 0.001]. Gender and years of experience in rehabilitation medicine were independent factors for the total score of the questionnaire. The total self-rating of female students was 9.65 points lower than that of the male (95%CI 4.386 to 14.914); and one more year in rehabilitation medicine would increase 1.78 points in self-rating (95%CI 1.140 to 2.419). ConclusionThe structure and content of RCF can be used to evaluate the competence of rehabilitation physicians. The teachers and students of NTPSRP are eligible to RCF. RCF-based evaluation can be used to guide the development of continuing education courses for rehabilitation professionals.

Development of traditional Chinese medicine rehabilitation curriculum based on World Health Organization rehabilitation competency framework / 中国康复理论与实践

ObjectiveTo develop a high-quality rehabilitation major curriculum using the World Health Organization rehabilitation competency framework (RCF), to improve the level of traditional Chinese medicine (TCM) rehabilitation major, to meet the requirements of competency-based education development. MethodsThe competence requirements of rehabilitation professionals of higher traditional TCM colleges and universities were analyzed using the theory and method of RCF. ResultsThe competency structure of TCM rehabilitation talents based on RCF was built, and the curriculum setting of TCM rehabilitation based on RCF was proposed. According to the characteristics of the educational environment of colleges and universities, a competency model suitable for undergraduate-level TCM rehabilitation major was established. ConclusionThe theory and methods of RCF are of great significance for the construction of competency-based education system of TCM rehabilitation major. Based on RCF, this study constructs the basic and practical curriculum system of TCM rehabilitation at the undergraduate level to promote the training of applied talents in TCM rehabilitation.

Application of Sendai Framework for Disaster Risk Reduction in Food Security During Flood Disaster in Malaysia: A Narrative Review

@#Disaster risk reduction (DRR) plays essential roles in ensuring food security to avoid adverse social, economic and financial implications to the disaster victims. This article aims to review existing DRR strategies in food security during flood disasters and recommend strategies that can be adapted into policies in Malaysia according to the Sendai Framework. Sendai Framework provides comprehensive, globally recognized priority themes in DRR while highlighting governmental and stakeholders’ responsibility. A literature review was carried out by searching available published literatures from online databases and selected information used in this review is taken from articles, journals, reports, national reports, news, and thesis regarding the topic from November 2021 to January 2022. This review revealed that DRR strategies of food security during flood disasters in Malaysia lack one out of 14 work areas in four priorities in the Sendai Framework, which is the national-level risk and vulnerability assessment system and tools. This article proposed 19 recommendations to improve eight work areas across all four priority areas to enhance national food security during flood disasters.

Construction and effectiveness evaluation of surgical complication monitoring mode based on medical record homepage data / 中华医院管理杂志

Objective:To explore the establishment of a surgical complication monitoring mode based on data on the medical record homepage, and analyze its impact on the trend of changes in surgical complication incidence.Methods:A monitoring mode of surgical complication was developed based on the " structure-process-results" framework by using surgical complication rates derived from performance appraisal for a tertiary general hospital in Guangzhou. The number of surgical complications and the number of discharged surgical patients was collected from the hospital from January 2019 to June 2022 through the home page collection system for performance appraisal of national tertiary public hospitals. Descriptive analysis was used to analyze the incidence of surgical complications, and Joinpoint regression was used to analyze the trend of changes in the incidence of surgical complications. Monthly percentage change ( MPC) and average monthly percentage change ( AMPC) were calculated. Results:Since the hospital began implementing the surgical complication monitoring mode in May 2021, the incidence of surgical complications had decreased from 2.55% in June 2021 to 0.82% in June 2022, with an MPC of -5.58% ( P=0.024), which was better than the changes from January 2019 to May 2021 ( MPC=0.18%, P=0.755). Conclusions:The surgical complication monitoring mode constructed by the hospital can effectively reduce the incidence of surgical complications, providing reference for optimizing hospital′s medical quality management process and decision-making mode.

A chiral metal-organic framework {(HQA)(ZnCl2)(2.5H2O)}n for the enantioseparation of chiral amino acids and drugs / 药物分析学报

Chiral metal-organic frameworks(CMOFs)with enantiomeric subunits have been employed in chiral chemistry.In this study,a CMOF formed from 6-methoxyl-(8S,9R)-cinchonan-9-ol-3-carboxylic acid(HQA)and ZnCl2,{(HQA)(ZnCl2)(2.5H2O)｝n was constructed as a chiral stationary phase(CSP)via an in situ fabrication approach and used for chiral amino acid and drug analyses for the first time.The{(HQA)(ZnCl2)(2.5H2O)}n nanocrystal and the corresponding chiral stationary phase were systematically characterised using a series of analytical techniques including scanning electron microscopy,X-ray diffraction,Fourier transform infrared spectroscopy,circular dichroism,X-ray photoelectron spectros-copy,thermogravimetric analysis,and Brunauer-Emmett-Teller surface area measurements.In open-tubular capillary electrochromatography(CEC),the novel chiral column exhibited strong and broad enantioselectivity toward a variety of chiral analytes,including 19 racemic dansyl amino acids and several model chiral drugs(both acidic and basic).The chiral CEC conditions were optimised,and the enantioseparation mechanisms are discussed.This study not only introduces a new high-efficiency member of the MOF-type CSP family but also demonstrates the potential of improving the enantiose-lectivities of traditional chiral recognition reagents by fully using the inherent characteristics of porous organic frameworks.

A metabolic intervention strategy to break evolutionary adaptability of tumor for reinforced immunotherapy

The typical hallmark of tumor evolution is metabolic dysregulation. In addition to secreting immunoregulatory metabolites, tumor cells and various immune cells display different metabolic pathways and plasticity. Harnessing the metabolic differences to reduce the tumor and immunosuppressive cells while enhancing the activity of positive immunoregulatory cells is a promising strategy. We develop a nanoplatform (CLCeMOF) based on cerium metal-organic framework (CeMOF) by lactate oxidase (LOX) modification and glutaminase inhibitor (CB839) loading. The cascade catalytic reactions induced by CLCeMOF generate reactive oxygen species "storm" to elicit immune responses. Meanwhile, LOX-mediated metabolite lactate exhaustion relieves the immunosuppressive tumor microenvironment, preparing the ground for intracellular regulation. Most noticeably, the immunometabolic checkpoint blockade therapy, as a result of glutamine antagonism, is exploited for overall cell mobilization. It is found that CLCeMOF inhibited glutamine metabolism-dependent cells (tumor cells, immunosuppressive cells, etc.), increased infiltration of dendritic cells, and especially reprogrammed CD8+ T lymphocytes with considerable metabolic flexibility toward a highly activated, long-lived, and memory-like phenotype. Such an idea intervenes both metabolite (lactate) and cellular metabolic pathway, which essentially alters overall cell fates toward the desired situation. Collectively, the metabolic intervention strategy is bound to break the evolutionary adaptability of tumors for reinforced immunotherapy.

Evaluation of the humoral immunity in mice induced by foot-and-mouth disease virus-like particles-ZIF-8 complexes with different sizes / 生物工程学报

To further enhance the immune effect of the foot-and-mouth disease (FMD) virus-like particles (VLPs) vaccine, this study prepared FMDV VLPs-zeolitic imidazolate (framework-8, ZIF-8) complexes with different particle sizes. We used a biomimetic mineralization method with Zn2+ and 2-methylimidazole in different concentration ratios to investigate the effect of size on the immunization effect. The results showed that FMDV VLPs-ZIF-8 with three different sizes were successfully prepared, with an approximate size of 70 nm, 100 nm, and 1 000 nm, respectively. Cytotoxicity and animal toxicity tests showed that all three complexes exhibited excellent biological safety. Immunization tests in mice showed that all three complexes enhanced the titers of neutralizing and specific antibodies, and their immune effects improved as the size of the complexes decreased. This study showed that ZIF-8 encapsulation of FMDV VLPs significantly enhanced their immunogenic effect in a size-dependent manner.

Immobilizing engineered Escherichia coli cells into zeolitic imidazolate framework 8 for efficient biosynthesis of Ala-Gln / 生物工程学报

The α-amino acid ester acyltransferase (SAET) from Sphingobacterium siyangensis is one of the enzymes with the highest catalytic ability for the biosynthesis of l-alanyl-l-glutamine (Ala-Gln) with unprotected l-alanine methylester and l-glutamine. To improve the catalytic performance of SAET, a one-step method was used to rapidly prepare the immobilized cells (SAET@ZIF-8) in the aqueous system. The engineered Escherichia coli (E. coli) expressing SAET was encapsulated into the imidazole framework structure of metal organic zeolite (ZIF-8). Subsequently, the obtained SAET@ZIF-8 was characterized, and the catalytic activity, reusability and storage stability were also investigated. Results showed that the morphology of the prepared SAET@ZIF-8 nanoparticles was basically the same as that of the standard ZIF-8 materials reported in literature, and the introduction of cells did not significantly change the morphology of ZIF-8. After repeated use for 7 times, SAET@ZIF-8 could still retain 67% of the initial catalytic activity. Maintained at room temperature for 4 days, 50% of the original catalytic activity of SAET@ZIF-8 could be retained, indicating that SAET@ZIF-8 has good stability for reuse and storage. When used in the biosynthesis of Ala-Gln, the final concentration of Ala-Gln reached 62.83 mmol/L (13.65 g/L) after 30 min, the yield reached 0.455 g/(L·min), and the conversion rate relative to glutamine was 62.83%. All these results suggested that the preparation of SAET@ZIF-8 is an efficient strategy for the biosynthesis of Ala-Gln.

Obesidade e interseccionalidade: análise crítica de narrativas no âmbito das políticas públicas de saúde no Brasil (2004-2021) / Obesity and intersectionality: critical analysis of narratives within public health policies in Brazil (2004-2021) / Obesidad e interseccionalidad: análisis crítico de narrativas en el ámbito de las políticas públicas de salud en Brasil (2004-2021)

Resumo: Objetivou-se realizar uma análise crítica da narrativa das políticas públicas de saúde brasileiras no cuidado da obesidade a partir de uma perspectiva interseccional. Trata-se de estudo qualitativo exploratório, documental e analítico, baseado na abordagem "What's the problem represented to be?" ["Qual é o problema representado para ser?"], conhecida como WPR. Tal abordagem se configura como uma ferramenta metodológica de análise crítica de políticas públicas a partir de seis perguntas norteadoras. Foram selecionados dez documentos, publicados entre 2004 a 2021 pelo governo brasileiro. A análise crítica resultou em três categorias: (i) causas da obesidade e narrativa dominante: quais são os problemas representados?; (ii) narrativa dominante e cuidado em saúde: quais são os efeitos para as pessoas com obesidade?; e (iii) obesidade e interseccionalidade: onde estão os silêncios? O consumo de alimentos e o sedentarismo foram a narrativa dominante como causas da obesidade. A interseccionalidade, mediada pelas categorias de gênero/sexo, raça/cor e classe social, foi identificada como um silêncio na narrativa das políticas públicas de saúde. Tais categorias não foram consideradas como causas atreladas à obesidade, tampouco foram incluídas de forma efetiva nas ações propostas pelas políticas públicas de saúde. Os silêncios encontrados no estudo destacam a necessidade de inclusão da interseccionalidade na elaboração e execução de políticas públicas de saúde e no cuidado das pessoas com obesidade. Tendo em vista as intersecções de gênero/sexo, raça/cor e classe social e suas formas de opressão com o surgimento e agravo da obesidade, são de extrema relevância análises críticas sobre as narrativas simplistas nas políticas públicas de saúde para problematização das lacunas que repercutem no cuidado dos usuários com obesidade.

Abstract: This study aimed to critically analyze the narrative of Brazilian public health policies in obesity care based on an intersectional approach. This is a qualitative exploratory, documentary, and analytical study based on the "What's the problem represented to be?" approach (WPR). This approach constitutes a methodological instrument for critical analysis of public policies based on six guiding questions. A total of ten documents were selected, published from 2004 to 2021 by the Brazilian government. The critical analysis resulted in three categories: (i) obesity causes and the dominant narrative: what problems are represented?; (ii) dominant narrative and health care: what are the effects for people with obesity?; (iii) obesity and intersectionality: where are silences? The consumption of food and sedentary lifestyle were the dominant narrative as causes of obesity. Intersectionality, mediated by the categories of gender/sex, race/skin-color, and social class, was identified as silenced in the narrative of public health policies, not being associated as linked causes of obesity, nor effectively included in the proposed actions of the policies. The silences found in the study highlight the need to include intersectionality in the elaboration and execution of public health policies and in the care of people with obesity. Considering the intersections of gender/sex, race/skin-color, and social class and their forms of oppression in the emergence and aggravation of obesity, critical analyses of simplistic narratives in public health policies are extremely relevant to problematize gaps affect the care of users with obesity.

Resumen: Este estudio tuvo como objetivo realizar un análisis crítico de la narrativa de las políticas públicas de salud brasileñas en el cuidado de la obesidad con base en un enfoque interseccional. Estudio cualitativo exploratorio, documental y analítico. Basado en el enfoque "Whats the problem represent to be?" [¿Cuál es el problema representado?], conocido como WPR. Tal enfoque se configura como una herramienta metodológica para el análisis crítico de las políticas públicas con base en seis preguntas rectoras. Se seleccionaron 10 documentos, publicados entre el 2004 y el 2021 por el gobierno brasileño. El análisis crítico resultó en tres categorías: (i) causas de la obesidad y la narrativa dominante: ¿Qué problemas se representan?; (ii) narrativa dominante y el cuidado en salud ¿Cuáles son los efectos para las personas con obesidad?; (iii) obesidad e interseccionalidad ¿Dónde están los silencios?. El consumo de alimentos y el sedentarismo fueron la narrativa dominante como causas de la obesidad. La interseccionalidad, mediada por las categorías de género/sexo, raza/color y clase social fue identificada como un silencio en la narrativa de las PPS, sin asociarlas como causas vinculadas a la obesidad ni incluirlas de forma efectiva en las acciones propuestas por las políticas públicas de salud. Los silencios encontrados en el estudio resaltan la necesidad de incluir la interseccionalidad en la elaboración y ejecución de las políticas públicas de salud y en el cuidado de las personas con obesidad. Considerando las intersecciones de género/sexo, raza/color y clase social y sus formas de opresión con el surgimiento y agravamiento de la obesidad, es sumamente relevante realizar análisis críticos sobre las narrativas simplistas en las políticas públicas de salud, para problematizar las brechas que repercuten en el cuidado de los usuarios con obesidad.

Intersectionality Theory, Challenges for Empirical Research and Contributions to Psychology / Teoria da Interseccionalidade, Desafios para Pesquisas Empíricas e Contribuições para a Psicologia / Teoría de la Interseccionalidad, Desafíos para la Investigación Empírica y Aportes a la Psicología

Abstract The Theory of Intersectionality is an important contribution from feminist epistemologies to the scientific field. This narrative literature review aims to discuss methodological possibilities and challenges in producing and analyzing empirical evidence based on the Theory of Intersectionality, as well as its contributions to Psychology. While this theory is increasingly being cited as the basis for empirical research, articulating its assumptions in knowledge production processes is still a difficulty. Qualitative approaches prevail in the field, but advancements in statistical analysis methods allow for an intersectional interpretation in quantitative studies. Intersectionality contributes to understanding psychological processes and challenging dominant and exclusionary assumptions in the field of Psychology. Embracing this theory requires a commitment to the imperative of social transformation and entails placing claims, values, practices, and power relations at the core of scientific knowledge production, regardless of the field under study.

Resumo A Teoria da Interseccionalidade é uma importante contribuição das epistemologias feministas para o campo científico. Este estudo de revisão narrativa da literatura teve como objetivo discutir possibilidades e desafios metodológicos na produção e análise de evidências empíricas embasadas na Teoria da Interseccionalidade e suas contribuições para a Psicologia. Embora essa teoria esteja crescentemente sendo citada como base de pesquisas empíricas, ainda se identifica a dificuldade de articular seus pressupostos nos processos de produção de conhecimento. As abordagens qualitativas são predominantes no campo e observa-se avanços em métodos de análises estatísticas que permitem uma leitura interseccional em estudos quantitativos. A interseccionalidade contribui para compreender processos psicológicos e desafiar suposições dominantes e excludentes no campo da Psicologia. Adotar essa teoria requer uma implicação com o imperativo de transformação social e implica tornar reivindicações, valores, práticas e relações de poder como cerne da produção de conhecimento científico, independentemente do campo estudado.

Resumen La Interseccionalidad es una importante contribución de las epistemologías feministas al campo científico. Este artículo de revisión narrativa tiene como objetivo discutir las posibilidades y desafíos metodológicos en la producción y análisis de evidencia empírica basada en la Interseccionalidad y sus contribuciones a la Psicología. Aunque esta teoría se cita cada vez más como base de investigaciones empíricas, aún se identifica la dificultad de articular sus supuestos en los procesos de producción de conocimiento. Los enfoques cualitativos predominan en el campo y se observan avances en métodos de análisis estadístico que permiten una lectura interseccional en estudios cuantitativos. La interseccionalidad contribuye a comprender los procesos psicológicos y desafiar suposiciones dominantes y excluyentes en el campo de la Psicología. Adoptar esta teoría requiere una implicación con el imperativo de transformación social e implica poner las demandas, valores, prácticas y relaciones de poder en el centro de la producción de conocimiento científico, independientemente del campo estudiado.