RESUMO
Peptic ulcer disease is a common condition of present day life, and its incidence is markedly increasing. The available drugs for the treatment are associated with adverse effects. Hence, there is a need for newer and better treatment for the same. Objectives: To evaluate the anti-ulcer activity of ethanolic extract of Vitis Vinifera (seed) by Water Immersion stress induced gastric ulcer model in Wistar albino rats using two doses (100, 200mg/kg body weight.) Methods: The antiulcer activity was assessed by estimating gastric volume, pH, free and total acidity; ulcer number and its inhibition, gastric lesion, ulcer severity and ulcer index; and comparing it with the control groups. Results: The present study revealed that grape seed extract of 100mg/kg and 200mg/kg showed a significant (p<0.05) reduction in the gastric volume, ulcer number, gastric lesion and severity. The ulcer index was 45.33 and 36.66 for 100 and 200mg/kg respectively. Ulcer inhibition for 100 and 200 mg/kg group was 32.79% and 44.86% when compared with the control group. Ulcer protection with 100 and 200mg/kg was 29.21% and 39.82% when compared with the control group. Conclusion: This present study indicates that Vitis vinifera seed extract have potential anti-ulcer activity in the model tested.
RESUMO
Introduction: Glycine max L. with Drakshasava, widely used by traditional healers as a formulation for the treatment of peptic ulcer in rural northern Karnataka in India, appears to be effective as assessed by patients and in our previously published research work of traditionally used formulation. Aim: The present study was undertaken to evaluate the safety and effi cacy of the overnight water soaked G. max grains. This is one of the components of traditional formulation. The study, approved by Institutional Animal Ethics Committee was carried out in male Wistar rats after assessing its toxicity in mice. Materials and Methods: Four groups of rats (n = 6 in each group) were treated with aspirin 200 mg/kg oral. In addition to aspirin control group received normal saline, standard group received 20 mg/kg omeprazole and 3rd and 4th group received G. max 250 and 500 mg/kg, respectively. All treatments were administered orally every 24 h for 7 days. After 24 hours fasting, on the 8th day stomach contents were aspirated under anesthesia to estimate free and total acidity. Stomachs were opened along the greater curvature to calculate ulcer index and subjected to histopathology studies. Statistics: The results were analyzed by one-way analysis of variance followed-by Dunnett’s post hoc test. P ≤0.05 was considered as signifi cant. Results: The severity of aspirin induced ulceration was found signifi cantly (P < 0.05) decreased in test groups compared with the control group. Free and total acidity was signifi cantly reduced in 500 mg/kg treated group, compared with the control group and was inferior to omeprazole treated group. Conclusion: The grain of G. max was found to be effective against aspirin induced ulcers.