Correlations of matrix metalloproteinase content and expression with invasion and metastasis of hepatocellular carcinoma / 吉林大学学报(医学版)

Objective To investigate the correlations of serum matrix metalloproteinase-2, -9 (MMP-2, MMP-9) contents and tissue expressions in hepatocellular carcinoma with tumor invasion and metastasis. Methods Serum MMP-2, MMP-9 contents were detected in 40 patient with hepatocellular carcinoma and 20 healthy controls by ELISA; the expressions and distributions of MMP-2 and MMP-9 in 40 patients and 10 normal tissues were detected by immunohistochemical method. Results Serum MMP-2, MMP-9 contents were significantly elevated in cancer samples compared with normal serum (P

Expression of matrix metalloproteinase-2 in patients with renal fibrosis and its clinical significance / 吉林大学学报(医学版)

Objective To study the change of matrix metalloproteinase-2 (MMP-2) in the development of renal fibrosis and evaluate the diagnostic value of blood MMP-2 level in renal fibrosis.Methods Thirty three patients with chronic glomerulonephritis were divided into proliferation group(n=18) and fibrosis group(n=15) according to renal fibrosis degree in renal pathology.Another 8 healthy subjects were used as control group.The levels of pro-MMP-2 and MMP-2 in serum were measured with the method of SDS-PAGE enzymography.Then the levels of pro-MMP-2 and MMP-2 in serum were compared between various groups.Results The serum MMP-2 level in proliferation group was much higher than that in control group(P0.05).Conclusion There is a significant difference of serum MMP-2 between suffering group and normal control group.Therefore the detection of serum MMP-2 level can be conducive to understand the development of disease and estimate prognosis.