RESUMO
By using histochemical reaction of Y-GTP,G-6-Pase and ATPase, an in vestiga-tion of alterations of Human Hepatocellular Carcincma(HCC)and its surrounding tissues was made. Liver tissues were obtained from 10 subjects at time of surgery. The experimental results showed that the enzymes significantly in HCC differed from those in its surrounding tissues. The Y-GTP activity in HCC was marked in 2 sub-lects, slight in 5 subjects,none in 3 subjects. The alterations mentioned above revealed no marked relation with the degree of HCC diferentiation .Thus Y-GTP could not be considered to be a reliable positive enzyme marker of HCC.The Y-GTP in its surrounding tissues showed increased staining intensity. G-6-Pase activity in HCC markedly decereased or disappeared, but increased markedly in its surrounding tissues. The staining of G-6-Pase could be used to distinguish cancerous from noncancerous areas. ATPase (Mg)aciivity in HCC decreased or disappeared as well.