RESUMO
Objective · To investigate ciliary beat frequency (CBF), and estrogen and progesterone receptor expression levels of human fallopian tubes after mifepristone treating in vitro. Methods · Human fallopian tube mucosa explants (n=25) were treated with different concentrations of mifepristone (0.1,1 and 10 μmol/L) or progesterone (10 μmol/L) separately, or both mifepristone and progesterone. After 24 h of treatment, CBF was measured. Quantitative real-time PCR and immunohistochemistry were used to research the expression of estrogen receptor-α (ERα) and progesterone receptor (PR) of human fallopian tubes after mifepristone treating. The ultrastructure of epithelial cells of fallopian tube after mifepristone (10 μmol/L) treating were observed with transmission electron microscopy. Results · The CBF at the concentrations of 0.1, 1 and 10 μmol/L was not affected by mifepristone (P=0.728,P=0.405 and P=0.941). The CBF decreased markedly in the group of 10 μmol/L progesterone compared to control group (P=0.000). Mifepristone (0.1,1 and 10 μmol/L) dose dependently antagonized the progesterone-induced CBF decrease (P=0.484, P=0.000 and P=0.000). Mifepristone upregulated the expression levels of ERα and PR in the fallopian tubes, but the ultrastructure of the cilia had no significant change. Conclusion · Mifepristone acts as progesterone antagonist in the human fallopian tube, which may explain the tubal contraceptive mechanism when used as an emergency contraceptive.
RESUMO
Objective · To investigate ciliary beat frequency (CBF), and estrogen and progesterone receptor expression levels of human fallopian tubes after mifepristone treating in vitro. Methods · Human fallopian tube mucosa explants (n=25) were treated with different concentrations of mifepristone (0.1,1 and 10 μmol/L) or progesterone (10 μmol/L) separately, or both mifepristone and progesterone. After 24 h of treatment, CBF was measured. Quantitative real-time PCR and immunohistochemistry were used to research the expression of estrogen receptor-α (ERα) and progesterone receptor (PR) of human fallopian tubes after mifepristone treating. The ultrastructure of epithelial cells of fallopian tube after mifepristone (10 μmol/L) treating were observed with transmission electron microscopy. Results · The CBF at the concentrations of 0.1, 1 and 10 μmol/L was not affected by mifepristone (P=0.728,P=0.405 and P=0.941). The CBF decreased markedly in the group of 10 μmol/L progesterone compared to control group (P=0.000). Mifepristone (0.1,1 and 10 μmol/L) dose dependently antagonized the progesterone-induced CBF decrease (P=0.484, P=0.000 and P=0.000). Mifepristone upregulated the expression levels of ERα and PR in the fallopian tubes, but the ultrastructure of the cilia had no significant change. Conclusion · Mifepristone acts as progesterone antagonist in the human fallopian tube, which may explain the tubal contraceptive mechanism when used as an emergency contraceptive.
RESUMO
10.3969/j.issn.1007-3969.2013.04.00X
RESUMO
The pharmacologic effects of verapamil (Ver) and phenylephrine(PE)on the smooth muscle of the human fallopian tube in vitro and their relationship to the stages of menstrul cycle were observed respectively. The results demonstrated that both the ampulla and isthmus of the human fallopian tube produced a contractive response to PE, which was antagonized by phentolamine. The PD_2 and PA_2 valucs were larger during the proliferative phase than during the secretory phase in the circular and longitudinal muscle of the isthmus. But there was no difference between the circular and longitudinal muscle. Ver supressed the activity of the human fallopian tuba smooth muscle and antagonized the effects of PE.The PD_2~' values were larger during the secretory phase than during the prolifertive phase. These pharmacologic characte risties of the tube indicated that d-adrenoceptor of the longitudinal muscle of the is thmus may be important, like the circular muscle in the regulation of ovum transport.