Sodium Phenylacetate Decreases the Immunosuppressive Factors Derived from Tumor Cells / 中国肿瘤生物治疗杂志

The immunosuppressive effect of supernatant of the cultured tumor cells, including breast adenocarcinoma ( MCF-7, MDA-453 ), gastrocarcinoma ( MKN-45 ), cervical cancer ( Hela ) and ovarian cancer ( 3AO ), was studied by MTT assay. The results showed that the supernatant of tumor cell culture suppressed the proliferation of lymphocytes activated with PHA. The supernatant of cultured tumor cells, except that of cultured MDA-453 cells, also suppressed the cytotoxic activity of LAK cells. It is suggested that there are immunosuppressive factors in the supernatant of tumor cell culture. But the immunosuppressive effect of supernatant derived from the cultured tumor cells treated with sodium phenylacetate that is a noncytotoxic differentiation inducer on the proliferation of lymphocytes activated with PHA and the cytotoxic activity of LAK cells , was decreased significantly. It indicates that sodium phenylacetate could decrease the immunosuppressive factors derived from tumor cells.

Effect of Lycium Barbarum Polysacch aride on Immunosuppressive Factors VEGF and TGF-?1in H_(22)-bearing Mice / 中药新药与临床药理

Objective To investigate the effects of Lycium barbarum polysaccharide(LBP)on the factors of immunologic escape.Methods LBP was given orally to H 22 -bearing mice for two weeks.The therapeutic effects of LBP were evaluat-ed by determining tumor weight ,thymus index and the inhibition rate of tumor growth.The levels of serum v ascular ep-ithelial growth factor(VEGF)and transforming growth factor beta1(TGF -?1)were measured by ELISA.Results LBP can inhibit tumor growth,protect thymus gland cells and decre ase the levels of serum VEGF \TGF -?1in H 22 -bearing mice.Conclusion The anti -tumor effect of LBP may be re lated to the inhibition of secretion of VEGF \TGF -?1in tuomr cells,and to the regulation of immunologic escape.