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OBJECTIVE To investigate the disinfectant-sulfanilamide resistance gene(qacE△1-sul1)and integrase gene(IntⅠ1)of Acinetobacter baumannii(ABA)isolated in pediatric clinic and analyze the relationship between these genes and multidrug resistance.METHODS Twenty eight strains of A.baumannii were collected and isolated from the sputum culture in the deep trachea of children with pneumonia during 2006.Identification of bacteria and susceptibility test by VITEK-32 automicroscan using GNI and GNS cards,were undertaken,qacE△1-sul1 gene and IntⅠ1 gene were analyzed by polymerase chain reaction(PCR).RESULTS Three of 28 strains of A.baumannii showed multidrug resistance,the positive rate was 10.71%.A.baumannii 4 strains were resistant to sulfamethoxazole/trimethoprim(SXT)with the positive rate 14.29%.Eleven strains that carrying qacE△1-sul1 genes and 4 strains carrying IntⅠ1 genes were detected,the positive rate was 39.29% and 14.29%,and qacE△1-sul1 and IntⅠ1 genes positive strains of A.baumannii were resistant to SXT,the other 7 qacE△1-sul1 positive strains were sensitive to SXT.CONCLUSIONS The main drug resistance in ABA resistant to SXT is to obtain qacE△1-sul1 gene.It should be paid attention to qacE△1-suⅠ1 positive but sensitive to SXT strains.It indicates that the strain carrying integron Ⅰ may show multidrug resistance.
RESUMO
OBJECTIVE To investigate the beta-lactamase gene and intⅠ1 gene in ESBLs-producing Klebsiella pneumoniae.METHODS Beta-lactamase gene and intⅠ1 gene were analyzed by PCR.RESULTS The positive rates of TEM,SHV,OKP,CTX-M-1group,CTX-M-2group,CTX-M-9group,GES,PER,VEB,OXA-10,ACT-1,LEN,DHA,and intⅠ1 were 51.4%,0%,0%,17.1%,0%,11.4%,0%,0%,0%,0%,0%,5.7%,17.1%,and 60%,respectively.Beta-lactamase gene was found in 62.9%,intⅠ1 gene was found in 60%.CONCLUSIONS There are very high positive percentages of beta-lactamase gene and intⅠ1 gene in ESBLs-producing K.pneumoniae.