Hematological Effects of Water Extracts of Cham-Dang-Gui on Dietary Induced Iron Deficient Anemia Rat

Cham-dang-gui (Angelica gigas nakai) has been used in traditional Korean medicine to treat hemotological disorders. The purpose of this study is to investigate the hematological effects of water extracts of Cham-dang-gui in rats with anemia induced by iron-free diets. Rats were divided into two groups, a regular diet group (RD) and an iron-free diet group (FeD). The iron-free diet group was then subdivided into the following three treatment groups: saline (1.0 ml/kgBW/day, FeDS), Cham-dang-gui (1.0 g/kgBW/day, FeDA), and iron (iron succinylate 14 mg/kgBW/day, FeDFe) groups. Rats were fed an iron-free diet for 6 weeks to induce iron-deficient anemia, and subsequently underwent the treatments, during which they were fed an iron-free diet for 3 weeks followed by a regular diet for 3 weeks. Body weights of the iron-diet groups (FeDS, FeDA, FeDFe) were lower than that of RD group. The blood levels of Hgb, Hct, MCV, MCH and TIBC were significantly higher in RD and FeDFe groups than those of other groups. The water extract of Cham-dang-gui with iron-free diet has no hematological effects. A regular diet followed by iron-free diets significantly increased blood levels of Hgb, Hct, and MCH.

Effects of Iron Supplementation on Iron Status of Anomic High School Girls / 대한지역사회영양학회지

This study was designed to investigate the effect of iron supplementation on the iron nutritional status and anemia of high school girls in Korea. One hundred thirty-five female students residing in Ulian metropolitan city in Korea diagnosed as having anemia or iron deficiency participated in this study. One or two tablets of iron medicine(80-160 mg Fe as ferrous sulfate/day) were administered to all participants for 3 months. Subjects were evaluated with a questionaire, measurement of hematological indices before and after iron supplementation. The average height and weight of respondents were 161.62 +/- 4.68 cm and 53.87 +/- 6.10 kg, respectively. Daily intakes of energy were 1597.8 +/- 302.35 kcal(76.0% RDA). Iron intakes were 13.72 +/- 4.17 mg (76.3% of RDA) and calcium intakes were 580.74 +/- 177.21(72.5% of RDA) before iron supp]ementation. At baseline, 63% of all participants had depleted store(serum ferritin 12 ug/ml and/or transferrin saturation(TS)<14%). After iron supplementation, this proportion declined to 19.3%. 55.6% of subjects had 12 ug/m1 of basal ferritin concentration before iron supplementation, and this proportion declined to 16.3% after iron supplementation. The basal hemoglobin(Hb) concentrations were 12.13 +/- 1.01 g/dl and they increased to 12.79 +/- 0.81 g/dl, which showed significant difference artier iron supplementation(p<0.001). The basal ferritin and TS(%) were 13.24 +/- 11.66 ng/ml, 18.42 +/- 10.12% and they significantly increased to 32.95 +/- 21.14 ng/ml, 33.53 +/- 16.64%, respectively(p<0.001). The basal total iron binding protein(TIBC) were 467.81 +/- 97.24 ug/dl and they significantly decreased to 325.05 +/- 48.89 ug/dl(p<0.001) after iron supplementation. The number of tablets administered was positively correlated with serum iron(t = 0.553, p<0.01), serum ferritin(t = 0.557, p<0.01), TS(%)(t = 0.588, p<0.01) and negatively correlated with TIBC(t= -0.409, p<0.01). The anemia symptoms such as 'Shortening of breath when going upstairs(p<0.01)', 'Tired out easily(p<0.01)', 'Feeling blue(p<0.001)', 'Decreased ability to concentrate(p<0.01)', and 'Poor memory(p<0.001)'improved significantly after iron supplementation. In this study, daily iron supplementations were efficacious in improving the iron status and anemic symptoms of female high school students. Regular check-ups and nutrition education for adolescents are necessary because of their vulnerability to iron deficiency. Further studies are needed to determine the minimum effective dose of iron and to examine the adverse effect of long-term iron supplementation.

A Study of Iron Status and Anemia in Female High School Students in Ulsan / 대한지역사회영양학회지

This study was designed to assess the iron nutritional status and anemia of high school students. 383 female subjects in Ulsan Metropolitan city were evaluated using a questionnaire, and a measurement of hematological indices. The average height and weight of the respondents were 161.24+/-4.90 cm and 53.12+/-6.37 kg, repectively. The average BMI(body mass index) was 20.43+/-2.26 which was in the normal ramge. The average hemoglobin(Hb) concentration of the subjects was 13.14+/-0.97g/dl, and the average hematocrit(Hct) level was 40.84+/-17.40%. Transferrin saturation{TS(%)} was 20.86+/-10.32%, and the ferritin by Hct(0.05)' and 'cold hands and feet' significantly(r= -0.109, p<0.05). The level of Mean corpuscular volume(MCV) was negatively correlated with 'feel dizzy when standing up', 'tired out easily', and 'decrease ability to concentrate' significantly(p<0.05). In particular, the level of Fe was negatively correlated with 'shortening of breath when going upstairs' and 'feeling blue' significantly(p<0.01). These results suggest That the prevalence of iron deficiency of female high school students is very high, therefore guidelin......

Study on the Correlation Between Iron Deficient Anemia and Pregnant Weeks in Gravida / 中国医师杂志

Objective To Study on regularity of iron deficient anemia(IDA) in gravida, provide preventive methods and improve hygienic quality.Methods IDA incidence rate of 12W～19W, 20W～27W, 28W～35W, 36W～pregnant duration were anlyzed in 521 gravida.Results The incidence of IDA in gravida was 16 87% and increased with the development of pregnancy.After 20 weeks of pregnancy,the incidence of IDA markedly increased accompanied with by the decreased Fe, SF and TS, especially during the 28～35 weeks of pregnancy, which reached the peak with the anenia incidence of 26 37%,and 80 50% of cases was irom deficient symptom,it suggesting iron deficiency in this stage and Fe, SF, TS decreased to the lowest level and the stored iron was almost exhausted and ZPP increased.Conclusions After 12 weeks of pregnancy,gravida should be supplemented with iron agent. It's also suggested that those gravida with gestational age older than 28 weeks with the Hb levels of less than 105g/L should be considered as IDA and should be treated with iron supplement. [

A Study on Iron Nutritional Status of Girls at Puberty in Kangnung Area / 대한지역사회영양학회지

This study was designed to assess the iron nutritional status of girls at puberty in Kangnung area. The subjects consisted of 161 adolescents in sixth-grade in primary school and first-grade in middle school girls. Anthropometric measurements were taken for body weight, height, percentage of body fat, and circumferences of waist and hip. Nutrient intakes were assessed by modified 24-hour recall method. Food models and other measuring tools were also used. Fasting blood samples were obtained and analyzed for hemoglobin(Hb) concentration, hematocrit(Hct), serum iron(FE) and total iron binding capacity(TIBC). Mean values for Hb, Hct, Fe, TIBC, TS and serum ferritin were 13.6+/-0.9g/dl, 39.6+/-3.9%, 91.3+/-36.3 microgram/dl, 327.9+/-45.2 microgram/dl, 28.3+/-11.8% and 37.4+/-24.2ng/ml, respectively. Prevalence of iron deficiency greatly varied by indices from 4.8% when judged by Hb to 18.4% by serum Fe concentration. The Hb concentration was positively correlated with Hct(r=0.641), serum iron(r=0.266) and transferrin saturation(r=0.237)(p<0.05). On the other hand, serum ferritin concentration showed significantly negative correlation with TIBC(r=-0.572). Mean daily intake of iron was 14.94mg and heme iron intake was 1.13mg and which was 7.6% of total iron intake. Total absorbable iron calculated by the method of Mosen was 1.38mg and bioavailability of diectary iron was 9.3%. These results suggest that the prevalence of iron deficiency of pubertal girls is very high, therefore the guidelines for diet and social supports, such as, school food service system should be provided to improve their iron status in middle school students.

Iron Nutritional Status of Female Students in Kangnung National University / 대한지역사회영양학회지

To evaluate iron nutritional status of female college students, fasting blood samples were taken from 76 female students of Kangnung National University. Hemoglobin(Hb), hematocrit(Hct), serum iron(Fe), total iron binding capacity(TIBC) and serum ferritin concentrations were measured and transferrin saturation was calculated. Mean values for Hb, Hct, Fe, TIBC, TS and serum freeitin were 13.64+/-1.42g/dl, 40.99+/-4.31%, 103.0+/-33.3 microgram/dl, 395.3+/-9.07 microgram/dl, 26.58+/-9.07%and 26.76+/-17.5ng/ml, respectively. Prevalence of iron deficiency greatly varied by indices from 6.8% when judged by Hct to 26.0% by serum ferritin concentration. The Hb concentration was positively correlated with hematocrit (r=0.5402), serum iron(r=0.2819) and transferrin saturation(r=0.2777)(p<0.05). on the other hand, serum ferritin concentration showed significantly negative correlation with TIBC(r=-0.3196). Two-day dietary intake records were collected from subjects to estimate mean daily iron intake and bioavailability of dietary iron. Mean daily intake of iron was 13.15 mg and heme iron intake was 0.83 mg which was 6.4% of total iron intake. Total absorbable iron calculated by the method of Monsen was 1.27 mg and bioavailability of dietary iron was 9.6%. In the light of high prevalence of iron deficiency based of serum ferritin concentration and low bioavailability of iron in the diet, guidelines about diet should be made to increase the content and bioavailability of iron in the diet if female college students.

A STUDY ON THE BIOAVAILABILITY OF HEME IRON IN RAT / 营养学报

In the present work, the bioavailability of heme iron has been studied by means of the hemoglobin regeneration method with iron-deficient rats model, which was conducted by feeding a low iron basal diet. Anemic rats were then divided into groups to which basal diets with various iron levels (7, 14, 28 ppm Fe) from heme iron (Group 1,2,3) and ferrous sulfate (Group 4, 5, 6, as a reference standard) were subsequently fed respectively.After 5-week regeneration period, the regenerated hemoglobin levels of the rats of corresponding groups were (1) 9.56→12.25, (2) 9.71→12.02, (3)9.51→ 11.09, (4) 9.74→10.81, (5) 9.66→11.24, and (6) 9.67→12.05, thereby the percentage of relative bioavailability (RBA) of heme iron were 117.7%, 124.8%, and 94.9% respectively. This result showed that the lower level of heme iron has a higher RBA. The reason why the effect of high level of heme iron was relatively lower was not fully understood.

IRON ABSORBABILITY FROM DIFFERENT DIETS IN IRON DEFICIENT ANEMIC CHILDREN BY NEUTRON ACTIVATION ANALYSIS OF A STABLE ISOTOPE (~(58)Fe) / 营养学报

The neutron activation analysis of a stable isotope (58Fe)was used successfully in the study of iron absorption from different diets in children suffering from iron deficient anemia. The method was correct and convenient, and uninjured to human body. The principle, procedure and the control of errors were also discussed. The iron absorbability of the balance diet,soy protein diet and soy protein diet plus vitamin-C were (group average?SD) 12.0? 3.6%, 10.7?4.7%, and 18,1?3.6% respectively. According to the results and the animal experiments which were reported in another paper, we agreed that soy protein unfavor or reduce the bioavailability of iron from diet, which was made by International Nutritional Anemia Consultative Group (INACG) and others, but it is still a disputing question. The data of iron absorbability from diets in children suffering from iron deficient anemia and the method of neutron activation analysis of a stable isotope (58Fe) have not be seen up to now in China. This paper will be valuable for studing, preventing and treating iron deficient anemia in our country.