RESUMO
Aim To study the regulatory of Jiaweisini-san on expression of hippocampal BDNF, NR1 and dental gyrus ( DG ) neurogenesis in rats with chronic stressed-depression and its possible mechamisms. Methods Chronic unpredictable mild stress was used to establish the rat model of stressed depression. The expression of BrdU, NeuN, brain -derived neurotro-phic factor ( BDNF ) and N-methyl-D-aspartate recep-tor1 ( NR1 ) in hippocampal dental gyrus were detected by fluorescently labeled immunohistochemical method. In addition, BDNFmRNA was detected by in situ hy-bridization. Results Chronic stress could inhibit the proliferation of neural precursors in hippocampal DG ( P<0. 01 );the expression of BDNF decreased signifi-cantly in DG in model rats ( P <0. 01 ) , while the ex-pression of NR1 increased significantly ( P <0. 01 ) . JWSNS and Fluoxetine hydrochloride significantly en-hanced the amount of new proliferating cells and the number of neurons in unit area of DG ( P<0. 01 ) , in-creased the expression of BDNF ( P <0. 01 ) and de-creased the expression of NR1 in DG(P<0. 01). Con-clusion JWSNS could promote the neuronal prolifera-tion in hippocampal DG of rat with chronic stressed-de-pression,and may exert an effect of promoting the pro-liferation of neurons in hippocampal DG by enhancing the expression of BDNF and decreasing the expression of NR1 .
RESUMO
Aim To study the effects of timeshare ad-ministration of Jiaweisinisan ( JWSNS ) on biological rhythm of rats with chronic stress. Methods Unpre-dictable mild chronic stress was used. Cosine rhythm analysis was adopted to observe and analyze the chan-ges of circadian rhythms of body temperature and se-cretion of 5-hydroxy tryptamine ( 5-HT ) , melatonin ( MT) and corticosterone ( CORT ) and the effects of JWSNS. Serum 5-HT,MT and CORT were detected by enzyme-linked immunosorbent assay. Results Three groups of administration of JWSNS had no inhibitory effects on weight gain in model rats,but improved the sucrose preference ( P Administration at 17 : 30 had biphasic regulatory effects on the peak and valley value,while administra-tion at 7 : 30 and 17 : 30 respectively mainly acted on the light phase,namely regulated the valley value. Rats with chronic stress showed delays of body temperature rhythm,namely postponed phase, which could only be inhibited by administration at 7 : 30 . There were de-creases in votatility and disorders in secretion rhythm, as was shown by dramatically accelerated phase of ser-um 5-HT, MT and CORT in rats with chronic stress. Administration of JWSNS at 17 : 30 or at 7 : 30 and 17 : 30 respectively had inhibitory effects on lower ser-um level of 5-HT in model rats ( P increase and the decreases in volatility. ConclusionAdministration of JWSNS at 7 : 30 achieves anti-de-pressant effects by regulating rhythms of hormones and neurotransmitters. Administration of JWSNS at 17 : 30 achieves anti-depressant effects by adjusting the secre-tion of hormones and neurotransmitters,while adminis- tration of JWSNS at 7 : 30 and 17 : 30 respectively a-chieves anti-depressant effects by those two above-men-tioned aspects.
RESUMO
Aim To study the anti-stress depression effect of Jiaweisinisan(JWSNS)and it's mechanism of N-methyl-D-aspartate(NMDA)receptor channel in hippocampus.Methods Chronic mild unpredictable stress(CMUS)was adopted to establish the rat depres-sion model.Before and after model establishing,the sucrose consumptions and the weight of rats were detected.TTC dyeing of hippocampus brain slices ex vivo were used to detect the situation of hippocampus neuron damage and the protective effect of serum containing components of JWSNS.The opening probability and the average opening time of NMDA receptor channels were detected by attach on cell patch clamp,the Ca2+ concentrations in single cell of hippocampus neurons were detected by Tillvis ION software.Results CMUS could decrease the sucrose preference and the weights of rats(P