Renoprotective Effect of Yi-Shui Sheng-Xin Yin on Mechanism among Spontaneously Hypertensive Rats / 世界科学技术-中医药现代化

This study was aimed to observe renoprotective effect and possible mechanism on Y i-Shui Sheng-Xin Yin in spontaneously hypertensive rats. Sixty 12-week male SHR rats were randomly divided into six groups , which were the Y i-Shui She ng-X in Y in low-dose group , middle-dose group , high-dose group , Benazepril group , model group and blank control group , and ten rats for each group . The SHR rats were sacrificed after eight weeks . The urine microalbumin , blood urea nitrogen and cystatin were tested in each rat . The HE and Masson staining method were used to observe changes of renal pathology . Changes of expression of transforming growth factor-β1 ( TGF-β1 ) , connective tissue growth factor ( CTGF ) , FN were detected by immunohistochemistry . The results showed that compared with the blank control group , blood pressure in model group was associated with a significant rise after 8 weeks. Compared with the model group, blood pressure in the Yi-Shui Sheng-Xin Yin middle-dose group, high-dose group and Benazepril group significantly decreased. Compared with the blank control group , urine microalbumin , blood urea nitrogen and cystatin in model group were associated with a significant rise . Compared with the model group , urine microalbumin , blood urea nitrogen and cystatin in the Y i-Shui She ng-X in Y in middle-dose group , high-dose group and Benazepril group significantly decreased . Pathological examinations showed that pathological changes in model group were faster than all drug-groups , appeared pathological changes of glomerular hypertrophy , glomerular basement membrane thickening of heterogeneity and extensive vacuoles degeneration . Immunohistochemical staining showed that compared with the blank control group , expressions of TGF-β1 , CTGF and FN of rat kidney tissue in model group were obviously up-regulated ( P < 0 . 05 ) . Compared with the model group , expressions of TGF-β1 , CTGF and FN in the Y i-Shui She ng-X in Y in , middle-dose group , high-dose group and Benazepril group were down-regulated ( P < 0 . 05 ) . It was concluded that Y i-Shui She ng-X in Y in can reduce SHR rats' early renal glomerulosclerosis and renal interstitial fibrosis , which play roles of delaying the progress of hypertension and protecting kidney . Its mechanism of action may be related to TGF-β1 , CTGF , FN signal pathways .

The relationship between gene expression of Toll-like receptor 2 and acute kidney lesion in mice with sepsis / 重庆医科大学学报

Objective:To observe the relationship of the expression of Toll-like 2 receptor(TLR2) with kidney injury in abdominal infection-induced sepsis.Methods:30 mice were randomly divided into 2 groups as follows: cecal ligation puncture group(CLP group,n=15) and sham operation group(control group,n =15).To adopt cecal ligation puncture(CLP) to establish the mice sepsis model,and the expression of TLR2 mRNA and TLR2 protein in kidney tissue were detected by reverse respectively.transcription and polymerase chain reaction(RT-PCR) and Western blo.The TNF?level in the kidney tissue and the creatinine(Cr)in the serum were also measured.Results:The expression of TLR2 mRNA in kidney tissues was markedly upregulated at 6h after CLP,peaked at 12h and kept in high level up to 24h,and the expression change of TLR2 protein was identical with the TLR2 mRNA expression.The expression of TLR2 mRNA was significantly correlated with TNF?and Cr levels.Conclusion:Severe infection could lead to marked up-regulation of TLR2mRNA and its protein expression in kidney tissue.There is a marked correlation between the changes of TLR2 expression and the actue kidney lesion.

A Clinical Analysis of Kidney Lesion in Myeloma / 中国医师杂志

Objective To analysis the clinical characteristics and the correlative factor of kidney lesion in multiple myeloma.Methods Analysis of statistics in clinical characteristics and results of laboratory of kidney lesion were carried out in myeloma patients.Results The incidence of kidney lision was 39.1%(18/46),the most common kidney lesion was chromic renal failure.There was a significant correlation between the quantity of bone marrow plasmacyte or urine Bence-Jones protein and renal functional lesion( P

Value of ultrasound signs in classification of the renal damage and lesion

137 patients with blunt renal trauma were examined by the sonography which can classify exactly 86.2% of renal injuries. Signs of the deformed kidney, of renal contour discontinuation and of the retroperitoneal hematoma were significantly for classification of renal injuries

Clinical Significance of Urinary Microprotein Examination on Early Diagnosis of Obesity - Related Glomerulopathy in Children / 实用儿科临床杂志

Objective To study the clinical significance of urinary mtcroprotems examination on early diagnosis of kidney damage of children with obesity. Method Urinary microproteins were tested in 40 obese children by enzyme - linked immunosorbent assay and 50 healthy children as controls. Results Urinary microprotem examination were abnormal in 8 obese children. Albumin in 5 cases increased, and transferrin(TRF) with retinol-binding protein in 1 case increased meanwhile. TRF in 3 cases increased. One case was abnormal by kidney biopsy.Conclusion Urinary microprotem examination was the sensitive index in early diagnosis of obesity-related glomerulopathy in children.