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Objective To study the effect of litchi saponin and flavones on glycometabolism in insulin resistance model of HepG2 cells .Methods Using high insulin in HepG2 cells to establish a cell model of insulin resistance .Cell model was treated by litchi saponin ,litchi flavones and rosiglitazone ,respectively .Glucose concentration of cell culture supernatant was detected by glucose oxidase method .Results In the concentration of 10-6 mol/L insulin for 24 hours ,HepG2 reached the highest level of resistant to insulin ,which means a successful insulin resistance cell model was established .However ,no significant decrease in glucose concentration of cell culture supernatant was observed with litchi saponin and flavones .Conclusion This study sug-gests that litchi saponin and flavones have no effect to improve insulin resistance .
RESUMO
OBJECTIVE:To investigate the mechanism of litchi saponin in impr oving action of insulin resistance(IR)in rats with hyperlipemia-hepar adiposum.METHODS:The hyperlipemia-hepar adiposum was used to establish IR model of rats,the effects of litchi saponin,roglitazone and gliclazide on the index of fasting serum glucose(FSG),total cholesterol(TC),triacylglycerol(TG),high density lipoprotein-cholesterol(HDL-C),tumor necrosis factor(TNF)-?,content of insulin,in?sulin sensitivity(ISI)and so on were observed in the model rats2hours after medication.RESULTS:Litchi saponin could sig?nificantly lower the level of FSG,TC,TG,contents of insulin and the concentration of TNF-?(P