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1.
Journal of China Pharmaceutical University ; (6): 452-466, 2022.
Artigo em Chinês | WPRIM | ID: wpr-939970

RESUMO

@#Fufang Shechuangzi Xiji (Lotion) is a common hospital preparation made from clinical prescriptions. Its clinical efficacy is accurate, yet its pharmacodynamic material basis has not been clarified.An ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) technique was developed to identify the chemical constituents of Fufang Shechuangzi Xiji (Lotion).The chemical constituents of Fufang Shechuangzi Xiji (Lotion) were scanned by electrospray ionization (ESI) source in positive and negative ion modes.As a result, a total of 118 compounds were identified and characterized via reference standards and by comparing mass spectrometry data with literature, including 45 alkaloids, 21 coumarins and chromones, 19 flavonoids, 14 saponins, 10 anthraquinones and 9 organic acids.As a result, UPLC-QTOF-MS/MS technology can quickly and sensitively identify the chemical constituents of Fufang Shechuangzi Xiji (Lotion), which provides a useful reference for exploring the pharmacodynamic material basis and further quality control study of the preparation.

2.
Journal of Pharmaceutical Practice ; (6): 437-441, 2021.
Artigo em Chinês | WPRIM | ID: wpr-886879

RESUMO

Objective To develop an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to simultaneously detect the contents of gallic acid, syringin, phellodendrine, aesculin and rhein in the gallnut lotion. Methods An UPLC- MS/MS method was established. Separation was performed on an Agilent Poroshell 120 EC-C18(2.1 mm×150 mm, 2.7 μm)with a gradient mobile phase system of 0.2% formic water-acetonitrile solution. The flow rate was 0.3 ml/min. The temperature of column was 30 ℃. The injection volume was 2 μl. The MS detection was in dynamic MRM mode. Results gallic acid, syringin, phellodendrine, aesculin and rhein were successfully separated using this method, with good linear relationship as the ranges of 153.8-15380、10.31-1031、5.265-526.5、50.70-5070、1.054-105.4 ng/ml, respectively. The precision, repeatability, stability and recovery were good. Conclusion This UPLC-MS/MS method is stable, rapid, and reproducible., It is suitable for detecting the contents of gallic acid, syringin, phellodendrine, esculetin and in the gallnut lotion.

3.
Journal of Pharmaceutical Practice ; (6): 118-120, 2021.
Artigo em Chinês | WPRIM | ID: wpr-875669

RESUMO

Objective To evaluate the transdermal safety of lanthanum (La) in sunscreen and jellyfish sting protective lotion, establish a microwave digestion-inductively coupled plasma opticalemission spectroscopy (ICP-OES) method for determination of lanthanum (La) in rat’s whole blood. Methods The whole blood samples were digested by microwave and analyzed by inductively coupled plasma emission spectrometer (ICP-OES). Using 333.749 nm as the analysis line, the content of La in rat whole blood was determined. Results The correlation linearity of the standard curve of this method was good (r>0.9994), the detection limit of the method was 0.0025 μg/ml, the limit of quantification was 0.0077 μg/ml, the precision was less than 3%, and the recovery rate was between 94.9% and 102.0%. Conclusion The ICP-OES method based on microwave digestion is stable and reliable, and can provide an important basis for the study of the transdermal safety of lanthanum.

4.
Cancer Research and Clinic ; (6): 651-656, 2021.
Artigo em Chinês | WPRIM | ID: wpr-912941

RESUMO

Objective:To investigate the inhibiting effect of Sufuning Lotion (SFN) on bladder carcinoma T24 cells.Methods:Trypan blue exclusion test was performed to observe the killing effect of 2 mg/ml SFN at different time points (20, 40, 60, 80, 100 min) on human bladder carcinoma T24 cells; the inhibiting effect of SFN with different concentrations (8.0, 12.0, 18.0, 27.0, 40.5 μg/ml) for 48 h on proliferation of T24 cells was assessed by using methyl thiazolyl tetrazolium (MTT) assay. The half inhibitory concentration ( IC50) was identified. T24 cells were treated with IC50 SFN for 24, 48, 72 h, and then the change of proliferation inhibition rate of T24 cells was detected. The nude mice subcutaneous model (30 mice) and intraperitoneal tumor xenograft model (30 mice) were prepared according to T24 cells inoculated method. After inoculation for 24 h, both animal models were divided into 5 groups with 6 animals in each group based on the random number method, including the control group (0.9% NaCl solution), the SFN 200 mg/kg group, the SFN 300 mg/kg group, the SFN 400 mg/kg group and the mitomycin group, and then the control group and three SFN groups were intraperitoneally injected for 6 d, while the mitomycin 1 mg/kg group was injected with 1 mg/kg mitomycin every 5 d for once, 2 times in total. The transplantable tumor volume of subcutaneous tumor xenograft model was measured per week and the mice were sacrificed after 4 weeks. Tumor tissues were taken out to measure the tumor weight and tumor growth inhibition ratio was also evaluated. The survival time of nude mice in intraperitoneal tumor xenograft model was recorded so as to calculate the life extension rate. Results:Trypan blue exclusion test showed that after the function of 2 mg/ml SFN for 20, 40, 60, 80, 100 min, the cell death rate was (17.83±1.56)%, (48.95±1.34)%, (67.46±1.44)%, (75.48±2.12)%, (89.41±1.35)%, respectively, and the difference was statistically significant ( F = 1 213.264, P < 0.01). MTT assay showed that SFN inhibited the proliferation of T24 cells in a concentration-dependent and time-dependent manner, and the IC50 of cell proliferation at 48 h was (14.36±0.35) μg/ml. After the function of 14.36 μg/ml SFN for 24, 48, 72 h, the proliferation inhibitory rate of T24 cells was (39.5±0.9)%, (50.6±0.7)%, (71.5±1.0)%, respectively, and differences was statistically significant ( F = 1 044.206, P < 0.01). After the nude mice was inoculated with T24 cells for 4 weeks, the tumor volume and tumor weight in the SFN 200 mg/kg group, the SFN 300 mg/kg group, the SFN 400 mg/kg group and the mitomycin group were lower than those in the control group [the tumor volume: (0.925±0.136) cm 3, (0.833±0.171) cm 3, (0.652±0.117) cm 3, (0.482± 0.120) cm 3 vs. (1.231±0.210) cm 3, respectively; the tumor weight: (1.56±0.20) g, (1.42±0.21) g, (1.19±0.22) g, (0.97±0.16) g vs. (1.98±0.30) g], and differences were statistically significant ( F = 20.153, P < 0.01; F = 17.325, P < 0.01); there were no significant differences in the tumor volume and weight between the SFN 400 mg/kg group and the mitomycin group ( t = 1.898, P = 0.069; t = 1.739, P = 0.094), the inhibition rate of subcutaneous tumor xenograft model was 20.94%, 28.28%, 39.66%, 51.14%, respectively in the SFN 200 mg/kg group, the SFN 300 mg/kg group, the SFN 400 mg/kg group and the mitomycin group. The survival time of intraperitoneal nude mice in the SFN groups and the mitomycin group was prolonged compared with that in the control group [(32.7±3.2) d, (34.0±4.5) d, (34.3±2.3) d, (35.3±2.0) d vs. (21.7±4.8) d], and there was a statistically significant difference ( F = 15.179, P < 0.01), the life extension ratio was 50.76%, 56.90%, 58.42%, 63.04%, respectively. Conclusion:SFN can inhibit the proliferation of T24 cells, and it has an anti-tumor effect on the T24-bearing nude mice.

5.
China Journal of Chinese Materia Medica ; (24): 4342-4349, 2019.
Artigo em Chinês | WPRIM | ID: wpr-1008200

RESUMO

The stable quality of hospital preparations is the basis for their clinical efficacy. Gynecological antipruritic prescription is widely used in gynecology clinics of Chinese medicine hospitals. Therefore,in this study,the production process of gynecological antipruritic lotion was optimized based on the concept of quality by design( QbD). The production process of the gynecological antipruritic lotion was developed to ensure its process stability and reliable quality,and enhance its clinical applicability. With total amount of matrine and oxymatrine used as the critical quality attribute( CQA) of the production process,parameter levels were designed based on production practice of hospital preparations,and Plackett-Burman and Box-Behnken experiments were used to optimize the water extraction and alcohol precipitation process of antipruritic lotion based on CQA of intermediates and final product. The soaking time,the first extraction time,and the second extraction time were determined as the critical process parameters( CPPs) of the production process. The optimal preparation process was as follows: water volume of 8 times,soaking for 0. 5 h,extraction for 2 times,the first extraction for 30 min,the second extraction for 56 min,alcohol concentration of 50%,and alcohol precipitation for 3 h. Furthermore,the design space was established based on the binomial regress model between CPPs and CQA,so as to set the optimization target and risk range; and the control space was displayed by overlay plot. The results of three repeated experiments in the control space showed that the relative standard deviation( RSD) of CQA was 4. 70%,and the similarity of chromatogram for gynecological antipruritic lotion was 0. 978,0. 974,and 0. 998,respectively. The above results indicated that the operation in the control space can guarantee the quality and stability of gynecological antipruritic lotion,suitable for practical application.


Assuntos
Antipruriginosos , Medicamentos de Ervas Chinesas , Água
6.
Chinese Traditional and Herbal Drugs ; (24): 3077-3083, 2019.
Artigo em Chinês | WPRIM | ID: wpr-851015

RESUMO

Objective: To establish HPLC fingerprint of antipyretic lotion for children and determine the content of 10 components (neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, forsythiae A, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, rosmarinic acid and forsythin). Methods: The analysis of antipyretic lotion for children was performed on the Kromasil 100-5 C18 (250 mm × 4.6 mm, 5 μm) chromatographic column, with mobile phase comprising of 0.1% phosphate acid-acetonitrile flowing at 1 mL/min in a gradient elution manner, and the column temperature was 35 ℃, and detection wavelength was set at 330 nm. With forsythia A as reference peak, HPLC fingerprint of 11 batches of preparations was established; Based on the conditions of fingerprint chromatogram, the content of 10 components was determined at the detection wavelength of 330 and 280 nm and the multi-index content of 11 batches of the preparation was determined. Results: HPLC fingerprint was established, a total of 37 peaks were selected as the common peaks, of which 22 peaks were identified, and the similarities of 11 batches of preparations were between 0.987 and 0.999. The linear relationships of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, forsythiae A, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, rosmarinic acid, and forsythioside were good in the range of 14.18-141.78, 20.53-205.63, 14.38-143.78, 5.62-56.19, 22.22-222.22, 8.40-83.98, 5.70-57.02, 7.46-76.36, 16.95-169.48, 8.59-85.94 g/mL, respectively. The average recoveries were 109.51%, 98.73%, 99.41%, 90.63%, 92.73%, 95.39%, 91.87%, 106.50%, 95.23%, and 108.71%. The content of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, forsythiae A, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, rosmarinic acid, and forsythin in the 11 batches were in the range of 306.38-457.85, 607.67-854.71, 306.81-469.02, 95.65-170.64, 484.41-819.44, 234.28-322.01, 145.42-226.85, 219.11-292.21, 347.94-507.74, 201.35-261.94 mg/mL, respectively. Conclusion: The method is accurate, simple, stable and reliable, which can be used for the quality control of antipyretic lotion for children.

7.
China Pharmacy ; (12): 3232-3239, 2019.
Artigo em Chinês | WPRIM | ID: wpr-817423

RESUMO

OBJECTIVE: To analyze chemical components in active fraction of Xiaozhong zhitong lotion, to clarify the material basis of its efficacy, and to provide reference for the second development of ointment preparation. METHODS: UPLC-Q-TOF-MS was adopted to analyze the chemical components of active fraction (40% ethanol elution site separated by D101 macroporous resin) of Xiaozhong zhitong lotion. The determination was performed on Hypersil GOLD aQ C18 column with mobile phase consisted of 0.1% formic acid acetonitrile (A)-0.1% formic acid water (B) (gradient elution) at the flow rate of 0.4       mL/min. The sample size was 4 μL, and the column temperature was 30 ℃. The condition of mass spectrometry was ESI detection in positive and negative scanning ion mode (ESI+/ESI-). The scanning range was 100-2 000 Da. The collision energy was 45/-45 eV, and the energy of the extended collider was 10/15 eV. The accurate molecular weight, retention time and multi-stage fragment ion information of the compounds were collected after obtaining the chromatogram, and the chemical components were identified by comparing with the mass spectrum information of reference materials and references. RESULTS: A total of 48 compounds were identified, and 9 and 39 compounds were identified under ESI+/ESI- ion mode, mainly including 10 phenolic acids, 8  phenylpropanoids, 9 anthraquinones, 3 flavones, 7 alkaloids, 5 tannins and 6 other categories. CONCLUSIONS: UPLC-Q-TOF- MS method is rapid, efficient and accurate for identify chemical components from active fraction of Xiaozhong zhitong lotion. Main chemical components of the active fraction are phenolic acids, phenylpropanoids, anthraquinones, alkaloids and tannins.

8.
China Pharmacy ; (12): 1801-1806, 2019.
Artigo em Chinês | WPRIM | ID: wpr-817235

RESUMO

OBJECTIVE: To screen active fractions of Xiaozhong zhitong lotion that are able to reduce swelling, promote ulcer healing and analgesia, and to provide reference for it’s secondary development of ointment preparation. METHODS: Water elution fraction and 20%, 40%, 60%, 95% ethanol elution parts were separated by D101 macroporous resin from Xiaozhong zhitong lotion. 120 SD rats were randomly divided into normal group (n=8) and modeling group (n=112). Rats in the normal group were not treated. Hemorrhoids model was established in the model group by injecting 75% glacial acetic acid into the perianal skin to induce perianal ulcer. 96 model rats were randomly divided into model group [blank ointment matrix 0.51 g/(kg·d)], positive group [Mayinglong ointment, 0.51 g/(kg·d)], high-dose and low-dose groups of Xiaozhong zhitong lotion and it’s water elution fraction and 20%, 40%, 60% ethanol elution parts [8.34, 2.78 g/(kg·d) by crude drug, all make into containing drug ointment], with 8 rats in each group. The periphery of the anus was smeared with relevant medicine, twice a day, for consecutive 7 d. The local symptoms around the anus of rats 3 and 7 days after administration and the pathological morphology of the local mucosa around the anus of rats 7 days after administration were observed and scored respectively. The effects of each elution part for reducing swelling and promoting ulcer healing were investigated. 120 ICR mice were randomly divided into model group [blank ointment matrix 1.03 g/(kg·d)], positive group [Mayinglong ointment 1.03 g/(kg·d)], high-dose and low-dose groups of Xiaozhong zhitong lotion and it’s each elution part [16.65, 5.55 g/(kg·d) by crude drug, all make into containing drug ointment], with 10 mice in each group. Transdermal administration, twice a day, for consecutive 7 d. After 30 min of last administration, the latency time and 15 min writhing times of mice were detected by designing acetic acid writhing test; pain threshold of mice was determined by hot-plate pain test so as to investigate systemic and local analgesic effects of each elution part. RESULTS: In the detumescence and ulcer healing test, compared with normal group, the score of local symptoms around anus at 3rd and 7th day of administration as well as pathological score of hemorrhoids local mucosa were increased significantly in model group (P<0.01). Compared with model group, above scores of positive group, 40% ethanol elution high-dose and low-dose groups were decreased significantly (P<0.05 or P<0.01). The scores of local symptoms around anus in 20% ethanol elution part high-dose group at 3rd and 7th day after medication as well as 60% ethanol elution part group at 7th day after medication were decreased significantly (P<0.05). In analgesia test, compared with model group, writhing latency time was shortened significantly and 15 min writhing times was decreased significantly in positive group, 40% ethanol elution part high-dose and low-dose groups as weel as 60% ethanol elution part high-dose group (P<0.05 or P<0.01); writhing latency time of 20% ethanol elution part high-dose group was shortened significantly (P<0.05). Pain threshold of mice was increased significantly in positive group, 40% ethanol elution part high-dose and low-dose groups after medication (P<0.05 or P<0.01). CONCLUSIONS: The 40% ethanol elution part from Xiaozhong zhitong lotion is the effective part that can reduce swelling, promote ulcer healing and analgesia.

9.
China Pharmacist ; (12): 151-153, 2018.
Artigo em Chinês | WPRIM | ID: wpr-705475

RESUMO

Objective:To establish an HPLC method with dual wavelengths for simultaneously determining chlorogenic acid , caffe-ic acid and paeoniflorin in Fufang Fuqing lotion .Methods: The HPLC method was performed on an Inertsil C 18 column ( 250 mm × 4.6 mm, 5 μm)with the column temperature at 40℃.The mobile phase was acetonitrile-0.02% phosphoric acid (17:83) with the flow rate of 1.0 ml· min-1 .The detection wavelengths were set at 323 nm and 230 nm.Results:The calibration curve of chlorogenic acid, caffeic acid and paeoniflorin showed a good linear relationship over the range of 7.50-120.00 μg· ml-1(r=0.9999), 2.50-40.00 μg· ml-1(r=0.9998) and 14.06-225.00 μg· ml-1(r=0.9997), respectively.The average recovery was 98.55% (RSD=1.66%,n=6), 94.52%(RSD=0.98%,n=6)and 99.18%(RSD=0.65%,n=6), respectively.Conclusion:The method is simple, quick, accurate and specific , which can be used for the quality analysis and control of Fufang Fuqing lotion .

10.
Journal of the Philippine Dermatological Society ; : 15-24, 2018.
Artigo em Inglês | WPRIM | ID: wpr-977656

RESUMO

Background@#Virgin coconut oil (VCO) has been reported to have anti-inflammatory and anti-pruritic properties and can be used as an alternative to corticosteroids for mosquito bites. No studies on VCO for mosquito bites have been published.@*Objective@#To compare the safety and efficacy of VCO against 1% Hydrocortisone as an anti-inflammatory and anti- pruritic preparation for mosquito bites.@*Method@#This is a randomized, double-blind study comparing the anti-inflammatory and anti-pruritic effect of VCO versus 1% Hydrocortisone on Aedes aegypti bites, by measuring the mean lesion size, subjective assessment of the effects on bites, pruritus intensity through the visual analog, and verbal rating scale in 91 subjects at baseline, 1 hour, days 1, 3, and 7.@*Results@#During the first hour and throughout the seven-day period, there was a decrease in the mean lesion size, visual, and verbal scale score for both VCO and Hydrocortisone groups. The mean lesion size for both groups were not statistically significant on the 1st and 24th hour. On day 3, the mean lesion size for the VCO group was 0.02 and 0.71 for the Hydrocortisone group which was statistically significant in favor of VCO. The mean visual and verbal scale scores for pruritus for both treatment groups were not statistically significant. As early as the 1st hour, the proportion of patients who reported total clearance of lesions in the VCO group was 34.09% compared to 6.38% in the Hydrocortisone group. On day 7, both treatment groups had resolution of lesions. No adverse reactions were noted.@*Conclusion@#Virgin coconut oil is safe, cost-effective, and comparable to 1% Hydrocortisone as an anti- inflammatory and anti-pruritic agent.


Assuntos
Óleo de Palmeira , Hidrocortisona , Anti-Inflamatórios
11.
Cancer Research and Clinic ; (6): 116-119, 2018.
Artigo em Chinês | WPRIM | ID: wpr-712778

RESUMO

Objective To investigate the efficacy and safety of intravesical instillation with Sufuning (SFN) lotion for prevention of postoperative recurrence of bladder cancer. Methods A total of 240 bladder cancer patients who were diagnosed as bladder cancer and accepted trans-urethral resection of bladder tumor from January 2010 to June 2016 in Shanxi Provincial Cancer Hospital were randomly divided into the experimental group (120 cases) and the control group (120 cases) according to the envelope method. The patients in the experimental group were treated with SFN lotion for immediate intravesical instillation(250 mg for once), and the patients in the control group were treated with pirarubicin (THP) for immediate intravesical instillation (30 mg for once). The patients of two groups were treated with intravesical chemotherapy once a week for 8 times, and the chemotherapy was performed once a month for 1 year. The recurrence rate, progression-free survival (PFS) rate, overall survival (OS) rate and recent side effects were compared between the two groups. Results The patients were followed up for 6 to 60 months. The median follow-up time was 36.5 months.In the experimental group,6 patients were lost and 8 patients were lost in the control group.The experimental group, the total recurrence rate was 26.3 % (30/114). The control group, the overall recurrence rate was 25.0 % (28/112) (χ2= 0.142, P = 0.781). Five years of PFS rate in the experimental group and the control group was 73.7 % (84/114) and 75.0 % (84/112) respectively, and there was no significant difference between the two groups (χ2= 2.011, P= 0.615). Five years of OS rate in the experimental group and the control group was 95.6 % and 92.9 % respectively, and there was no significant difference between the two groups (χ 2= 1.611, P= 0.425). The major side effects included chemical cystitis and hematuria. The incidence of chemical cystitis and hematuria in the experimental group was significantly lower than that in the control group(χ2=5.991,P=0.018;χ2=4.925,P=0.036).There was a statistically significant difference of the hematological side effects (blood routine changes) between the two groups (χ 2= 4.891, P= 0.032). Conclusion It is safe and effective for intravesical instillation of SFN lotion to prevent the recurrence of bladder cancer.

12.
Herald of Medicine ; (12): 1374-1376, 2017.
Artigo em Chinês | WPRIM | ID: wpr-667686

RESUMO

Objective To discuss the clinical therapeutic effect of Kangmin xiaoyan lotion combined with levocetirizine hydrochloride in the treatment of facial recurrent dermatitis. Methods Eighty cases of facial recurrent dermatitis were randomly divided into treatment group and control group,40 cases in each group.The treatment group was given Kangmin xiaoyan lotion with wet compression for 20 min in the morning and evening,and levocetirizine hydrochloride 10 mL orally once daily;the control group was only given levocetirizine hydrochloride orally once daily.One month later,the symptoms and signs of skin lesions and adverse reactions in each group were observed. Results One month after the treatment,the scores of symptoms and signs were significantly decreased in the two groups (P<0.05).After the treatment,the scores were significantly different between the two groups (P<0.05).Clinical efficacy of the treatment group and control group was 92.5% and 65.0%,with significant difference(P<0.05). Conclusion Kangmin xiaoyan lotion can be effective in the treatment of facial recurrent dermatitis,and it is worthy of clinical application.

13.
Drug Evaluation Research ; (6): 1432-1435, 2017.
Artigo em Chinês | WPRIM | ID: wpr-664745

RESUMO

Objective To establish a method for the microbial limit test of zinc oxide and talcum powder lotion in Chinese Pharmacopoeia 2015.Methods Microbial enumeration test and specified microorganisms test with instructions were conducted from Chinese Pharmacopoeia 2015,which involved 10 batches zinc oxide and talcum powder lotion and five species of bacteria in all.The samples were treated by centrifugation and membrane filtration.Microbial enumeration test:the total number of aerobic bacteria using TSA medium to examine,strains were Staphylococcus aureus,Pseudomonas aeruginosa,Bacillus subtilis,Candida albicans,and Aspergillus niger;The total number of molds and yeasts using SDA culture medium to examine,strains were Candida albicans and Aspergillus niger.Each strain was divided into two groups:Adding bacteria test group and bacteria control group.Samplecontrol group and negative control group of each culture medium was prepared respectively.Count the colonies and calculate the rate of recovery.Specified microorganisms test:Pseudomonas aeruginosa and Staphylococcus aureus were applicated and set the bacteria validation group,sample control group,and negative control group.The culture medium of each group was crossed on the corresponding medium plate,and Microflex LT mass spectrometer was used to identify the bacteria.Results The recoveries of all kinds of strains in microbial enumeration test,total yeast and mold count were 0.75-1.16 in all batches.In the validations of Staphylococcus aureus and Pseudomonas aeruginosa,all kinds of strains were respectively detected in bacteria validation group,and there were no bacterial growth in sample control group and negative control group.Conclusion The microbiological examination methods for zinc oxide and talcum powder lotion can meet the requirements of Chinese Pharmacopoeia 2015.

14.
China Pharmacist ; (12): 463-465, 2017.
Artigo em Chinês | WPRIM | ID: wpr-510006

RESUMO

Objective:To establish the HPLC fingerprint detection method for gynecological lotion. Methods:Wondasil C18 (250 mm × 4. 6 mm,5 μm)was selected as the analytical column. The mobile phase was composed of acetonitrile-0. 3% phosphric acid and 0. 3% diethylamine solution with gradient elution at a flow rate of 1. 0 ml·min-1 . The detection wavelength was set at 284 nm and the column temperature was 30℃. Ten batches of gynecological lotion were detected by the HPLC fingerprint and evaluated by Chromato-graphic Fingerprint Evaluation System of Chinese Medicine (2004 edition). Results: The separated peaks were clear and 15 common peaks were identified in the fingerprint of 10 batches of gynecological lotion. Conclusion: The HPLC fingerprint is with good repeat-ability and stability, which can provide evidence for the quality control of gynecological lotion.

15.
China Pharmacist ; (12): 368-371, 2017.
Artigo em Chinês | WPRIM | ID: wpr-507551

RESUMO

Objective:To establish the quality standard for infantile bronchitis traditional Chinese medicine lotion. Methods:TLC was used for the qualitative identification of Ephedra herba, Paeonia lactiflora Pall. , Poria cocos, Fructus aurantii and Epimedium brev-icornu Maxim. . HPLC was used for the content determination of ephedrine hydrochloride, pseudoephedrine hydrochloride and paeoni-florin. The chromatography conditions for ephedrine hydrochloride and pseudoephedrine hydrochloride were as follows: an AElichrom Pdar-Phenyl Polyphenyl ether-bonded phenyl-bonded silica column (250 mm × 4. 6 mm, 5μm) was used, the mobile phase was aceto-nitrile-0. 2% phosphoric acid solution (1 :99), the flow rate was 1. 0ml·min-1, the detection wavelength was 210 nm, the column temperature was 30℃ and the injection volume was 10 μl. The chromatography conditions for paeoniflorin were as follows:an Inertsil ODS-3 C18 column (250 mm × 4. 6 mm, 5 μm) was used, the mobile phase was acetonitrile-0. 1% phosphoric acid solution (15:85), the flow rate was 1. 0 ml·min-1, the detection wavelength was 230 nm, the column temperature was 30℃ and the injection vol-ume was 10 μl. Results:The TLC results of Ephedra herba, Paeonia lactiflora Pall. , Poria cocos, Fructus aurantii and Epimedium brevicornu Maxim. showed clear spots with good resolution. Ephedrine hydrochloride, pseudoephedrine hydrochloride and paeoniflorin had a good linear relationship within the range of 10.11-101.10 μg·ml-1(r=0.9996), 10.08-100.80 μg·ml-1(r=0.9991) and 20.50-102.5 μg·ml-1(r =0.9996), respectively. The average recovery was 98.80% (RSD =1.87%, n =6), 98.77%(RSD=1. 72%, n=6) and 99. 57% (RSD=1. 56%, n=6), respectively. Conclusion: The established quality standard can be used for the quality control of infantile bronchitis traditional Chinese medicine lotion.

16.
Chinese Journal of Information on Traditional Chinese Medicine ; (12): 87-90, 2017.
Artigo em Chinês | WPRIM | ID: wpr-507389

RESUMO

Objective To screen the presciption of Blumea gynecological lotion and investigate the inhibitory effect through inhibition rate test; To provide a basis for clinical application. Methods Consumption of Tween-80, SLES, hydrogenated castor oil (RH-40), and clarity of the solution were selected as the evaluation indexes to optimize the solubilizing agents. By using the filter paper method and the inhibition zone size as the evaluation index, the antimicrobial effect on Staphylococcus aureus. Escherichia coli and Candida albicans was studied to screen the best antibacterial agent from 2 g/L GM-BP, 2 mL/L PHMB, 2 mL/L A.SAP. With Blumeapowder dissolution percentage as the evaluation index, orthogonal test was used to screen additives in the lotion. Finally, the bacteriostasis effect and stability of the obtained lotion were investigated in vitro. Results The order of solubilizing effect was RH-40>SLES>Tween-80, and that of inhibition zone size was PHMB>A.SAP>GM-BP. The best prescription as follows:0.6 mg/mL Blumea powder, 0.04 g/mL SLES, 0.01 g/mL CAB-35, 10% propylene glycol. Bacteriostatic rates of Blumea gynecology lotion sample were more than 90%, and bacteriostatic rates remained more than 80% after 3 months. Conclusion The prescription of Blumea gynecology lotion is reasonable and practicable, with simple preparation process, available materials and good inhibitory effect and stability.

17.
Journal of Pharmaceutical Practice ; (6): 441-443, 2017.
Artigo em Chinês | WPRIM | ID: wpr-790789

RESUMO

Objective To develop a method for the determination of total anthraquinones in Xiaofan lotion by HPLC.Methods Rhubarb anthraquinones were simultaneously separated and assayed on a Kromasil C18 column(250 mm×4.6 mm,5 μm) with the mobile phase of 0.1%phosphoric acid-methanol (15∶85) at 30 ℃.The detection wavelength was 207 nm.Results The linear relationship is good for Aloe-emodin and other five standard ingredients.The average recovery was 99.45%, RSD 1.68%.Conclusion The method is simple, rapid and accurate.It is suitable for determination of total anthraquinones in Xiaofan lotion.

18.
Chinese Traditional and Herbal Drugs ; (24): 3370-3376, 2017.
Artigo em Chinês | WPRIM | ID: wpr-852593

RESUMO

Objective: Through the accelerated experiment and long-term experiment, the main contents of ephedrine and white peony root in Chinese materia medica (CMM) lotion were taken as the main indexes, and the stability of the medicine lotion of infants with bronchitis was investigated. Methods: According to the quality standard of CMM lotion for infant bronchitis, the existence of Ephedrae Herba, Poria, Paeoniae Radix Alba, Aurantii Fructus and Epimedii Folium in the sample was identified by thin layer chromatography. The contents of Ephedrae Herba (ephedra hydrochloride and pseudoephedrine hydrochloride) and Paeoniae Radix Alba (paeoniflorin) were determined by HPLC, and the stability of CMM lotion samples was evaluated by plate method. Results: The contents of Ephedrae Herba, Poria, Paeoniae Radix Alba, Aurantii Fructus, and Epimedii Folium were measured. The contents of Ephedrae Herba and Paeoniae Radix Alba were higher than 0.18 mg and 0.72 mg; The total contents of aerobic bacteria and mycorrhizal yeasts were not more than 100 cfu/mL, and Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus were not detected, in line with quality standards. Conclusion: The three batches of CMM lotion prepared by the First People’s Hospital of Guangxi have good stability and long-term stability, for the goods packaging, transportation, and storage conditions to provide the necessary information.

19.
Journal of Bacteriology and Virology ; : 41-53, 2017.
Artigo em Inglês | WPRIM | ID: wpr-225910

RESUMO

Purpose of this study was to evaluate the influence of a lotion on the bacterial community in the human forearm skin. The chemical- and natural-based lotions were applied on the left and right inner forearm skins, respectively, of 14 participants, who cleansed forearm skin using sterilized cotton swabs. The germs on cotton swabs were analyzed using libraries of PCR amplicons. The genetic diversity of the bacterial communities detected on the natural-based lotion-applied skin (NLS) was significantly higher than that of the bacterial communities on the chemical-based lotion-applied skin (CLS) in all participants, except two. The diversity was estimated based on operational taxonomic unit (OTU), Chao1, Shannon, and Simpson indices. Bacterial communities obtained from the CLS and NLS were phylogenetically separated into 5 and 3 monophyletic groups, respectively, based on lotion types. The taxonomic distribution of the bacterial communities, which were composed of 198 genera in 14 phyla in the CLS and NLS, respectively, was irregularly and biasedly separated into 2 groups based on the lotion types. Among the 14 phyla, Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria were found to be relatively dominant, and 15 of the 198 genera, including Methylobacterium, Propionibacterium, Pseudomonas, Staphylococcus, Streptococcus, and Bacillus were relatively dominant (>0.5%). The taxonomic distribution of dominant bacterial communities from CLS and NLS was irregularly and biasedly separated without relation to the lotion types. In conclusion, the chemical- and natural-based lotions were responsible for changing or influencing the genetic diversity, phylogenetic separation, and taxonomic distribution of skin bacterial communities.


Assuntos
Humanos , Actinobacteria , Bacillus , Bacteroidetes , Firmicutes , Antebraço , Variação Genética , Methylobacterium , Reação em Cadeia da Polimerase , Propionibacterium , Proteobactérias , Pseudomonas , Pele , Staphylococcus , Streptococcus
20.
Herald of Medicine ; (12): 1294-1297, 2017.
Artigo em Chinês | WPRIM | ID: wpr-661338

RESUMO

Objective To establish a specific HPLC method for simultaneous determination of three components ( baicalin, linarin and rhein) in Cuochuang Xiaoyan lotion. Methods The three components in Cuochuang Xiaoyan lotion were assayed by HPLC gradient elution method.The assay was performed with Waters Xterra MS C18(4.6 mm×250 mm,5 μm) column, with acetonitrile (A) and 0.2% phosphoric acid solution (B) as mobile phase in gradient elution.The flow rate was 1.0 mL·min-1.The detection wave length was 277 nm and column temperature was 30 ℃. Results Three components were completely separated from the adjacent peaks and a good linear relationship between each sample concentration and integral area was obtained.The linear equations were as follows:Ybaicalin=9.208X-0.0994(R2=0.9999, 83.97-839.70 μg·mL-1);Ylinarin=3.0628X-0.0038 ( R2 = 0. 9999, 34. 75-347. 49 μg · mL-1 );Yrhein = 1. 0225X-0. 0286 ( R2 = 0. 9998, 63. 20-632.00 μg·mL-1 ) . Conclusion The HPLC method is simple, accurate and reproducible, which is effective in controlling the quality of Cuochuang Xiaoyan lotion.

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