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BackgroundThese study made us to investigate the drug row material of Mongolian traditional medicine such us Tribulus terrestris, Malva neclecta Wall and Eriocheir sinensis compounded preparation named “Malbus”. Investigated us preparations steel using for treatment of kidney and urinetract deseases, it is one of effective in Mongolian traditional drug preparations.Material and MethodsThe experimental protocol was approved by the Ethics Review Committee at the Ministry Health of Mongolia. In research we used 20 healthy mice and 60 Wistar rats. Investigation was based and implemented at scientific research laboratory of Traditional Medical Science Technology and Producttion Corporation and pathological laboratory of Institute Veterinary medicine. Chemically acting substances is steroid saponin content in Malbus compound was detected by using thin layerchromatography (TLC) and its value was determined by UV-spectrophotometry. The acute oral toxicity study was according to the method Prozorovsky (1978). The toxic nephrosis was produced using Gentamicin (80 mg/kg) rats by using Neugarten’s method (1983).ResultsThe ethanolic extract results of the phytochemical investigations showed that conteined in the “Malbus” determined the presence of bioactive substances such as flavonoids, steroid saponins. The ethanolic extract of Malbus was found to be LD50 of 16.3 g/kg. Toxic nephrosis was induced in Wistar rats administered preparation Malbus dose 80 mg/kg, 160 mg/kg, and 240 mg/kg. Blood levels of creatinine, uric acid, and urea were siginificantly reduced by Malbus treatment compared tocontrol. Histological study revealed that Malbus was effective for treatment of nephritis in rats induced by gentamicin.Conclusions:1. The ethanolic extract of “Malbus” compound determined the presence of bioactive substances such as flavonoids, steroid saponins.2. LD50 of Malbus 16.3 g/kg, the preparation is has nephroprotective effect on experimental Gentamicine induced nephrosis in rats.
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Objective To investigate the localization and distribution of the five endocrine cells in the digestive tract mucosa of ricefield eel(Monopterus albus). Methods Using immunocytochemical technique of strept avidin-biotin-peroxidase complex(SABC) were used. Results At least 5 kinds of immunoreactive endocrine cells distributed in the digestive tract mucosa of M.albus. They were gastrin(Gas),somatostatin(Som),5-hydroxytryptamine(5-HT),insulin(Ins),and neurofilament (NF) immunoreactive endocrine(IRE) cells.Gas and Som-IRE cells distributed between stratified squamous epithelium and goblet cell in esophagus. A large number of Gas-IRE cells were found between gastric fundus epithelium and gastric glands, and only a few in the carcia. Ins, 5-HT and NF-IRE cells distributed in the epithelium pylorus and pyloric glandular tube respectively. No any immunoreactive positive reaction was found in the gut of M.albus.In addition, immunoreactive positive reaction of glucagons was not found in whole digestive tract.All immunoreactive endocrine cells were dark brown in color.Their morphology was irregular, cytoplasmic process was shorter and thicker, their nucleus showed an empty bubble.They distributed between esophageal epithelium and gastric epithelium or glandular epithelium, and cytoplasmic process extended to the gastric lumen and glandular cavity.Conclusion There is a complex endocrine function of the digestive system in ricefield eel (M.albus) at the lowest vertebrate.