Network pharmacology study of Tibetan medicine Corydalis Herba against acute myocardial ischemia / 中国中药杂志

In this study, the compound search was completed through SciFinder and CNKI databases, and the drug-like properties were screened in FAFdrugs4 and SEA Search Server databases. In addition, based on the target sets related to acute myocardial ischemia(AMI) searched in disease target databases such as OMIM database, GeneCards database and DrugBank, a network diagram of chemical component-target-pathway-disease was established via Cytoscape to predict the potential active components of Corydalis Herba, a traditional Tibetan herbal medicine which derived from the aerial parts of Corydalis hendersonii and C. mucronifera against AMI. A protein-protein interaction(PPI) network was constructed through the STRING database and the core targets in the network were predicted. And the enrichment analyses of core targets were completed by DAVID database and R software. Furthermore, a molecular docking method was used to verify the binding of the components with core targets using softwares such as Autodock Vina. The present results showed that there were 60 compounds related to AMI in Corydalis Herba, involving 73 potential targets. The GO functional enrichment analysis obtained 282 biological processes(BP), 49 cell components(CC) and 78 molecular functions(MF). KEGG was enriched into 85 pathways, including alcoholism pathway, endocrine resistance pathway, calcium signaling pathway, cAMP signaling pathway, vascular endothelial growth factor signaling pathway and adrenergic signaling transduction pathway of myocardial cells. The results of network topology analysis showed that the key components of anti-AMI of Corydalis Herba might be tetrahydropalmatine, etrahydrocolumbamine, N-trans-feruloyloctopamine, N-cis-p-coumaroyloctopamine, N-trans-p-coumaroylnoradrenline and N-trans-p-coumaroyloctopamine, and their core targets might be CDH23, SCN4 B and NFASC. The results of molecular docking showed that the key components of Corydalis Herba had stable binding activity with the core targets. This study provides reference for further elucidation of the pharmacological effects of Corydalis Herba against AMI, subsequent clinical application, and development.

Consideration on critical quality attributes determination of Chinese materia medica preparations / 中草药

In this paper, through the analysis of the key issues in the confirmation of the critical quality attributes (CQAs) of traditional Chinese medicine preparations, it is proposed that the determination of the CQAs should fully consider factors such as the efficacy, safety, nature of the drug substance, requirements of formulation and overall requirements of drug quality control. Complete quality control items that can characterize the properties of drugs should be extracted to provide a basis for the establishment of process control and drug quality standards.

Study on Material Basis of Miao Medicine Bi-Ning Spray in Allergic Rhinitis Treatment / 世界科学技术-中医药现代化

This paper was aimed to study the material basis for the efficacy of Miao medicine Bi-Ning spray in the treatment of allergic rhinitis,and to lay a foundation for improvement of its quality standard.Solidago and Centipeda minima were weighed according to the prescription and then soaked in water.Reflux extraction was conducted to the liquid.Petroleum ether,ethyl acetate,and n-butanol were used in the extraction,respectively.Each received composition was dried and made into sample solution for the experiment.A total of 60 healthy adult SD rats,with the ratio of half male and half female,were selected and randomly divided into 6 groups according to the weight with 10 rats in each group.There were the normal group,the model group,the petroleum ether (SYM) group,the ethyl acetate (YSYZ) group,the nbutyl alcohol (ZDC) group and the water group.The 20％ xylene olive oil solution was used in the model establishment of rats from different groups except the normal group.Intranasal administration of drug was given to different dosage group after the models were successfully established.Normal saline was given to the normal group and the model group.Serum of each experimental rat was collected at the end of the experiment.The contents of IgE,IL-4 and HT in serum were detected by ELISA.The pathological morphology of the nasal mucosa was analyzed.The results showed that compared with the normal group,the contents of IgE,IL-4 and HT in serum of the model group increased significantly (P ＜ 0.05).Compared to the model group,the contents of IgE,IL-4 and HT in serum of the water group decreased significantly (P ＜0.05).Contents of IgE,IL-4 and HT in other groups presented decreasing tendency.However,there was no statistical significance.The pathological morphology of the nasal mucosa results showed that compared with the normal group,the cell inflammatory response of the nasal mucosa tissues significantly increased in the model group (P ＜ 0.01).Compared with the model group,the cell inflammatory response of nasal mucosa tissues significantly decreased in the ZDC group and water group (P ＜ 0.05).There was no statistical significance in other groups.It was concluded that components in water part were the main material basis for efficacy of Bi-Ning spray in the treatment of allergic rhinitis.

Current Progress of Potassium Nutrition on Quality in Chinese Herbs / 浙江中医药大学学报

[Objective]To provide a reference for the effects of potassium on quality formation of medicinal material. [Methods] Withpotassium,traditional Chinese medicine,growth and development,physiological characteristicsandeffective medicinal substancesas the key words, from June 2012 to March 2017 in the Pub Med, CNKI, WANFANG, VIP, the effects of potassium on the growth and development, production and quality formations, plant physiology, resistance acquirement, medicinal substances in medicinal material were systematically summarized. [Results] A total of 71 articles were retrieved, of which there were 50 valid literatures. At present, the related researches mainly focus on the relationship between potassium and growth and development, yield and quality formation, effective medicinal substances metabolism of Chinese herbs. [Conclusion] Some progresses have been made in this field. But this paper pointed out the problems in this field and put forward the directions about further research of effective medicinal substances metabolism and its mechanism, establishing multiple factor, multilevel nolinear synthesis model.

Quality Assessment on Fructus Polygoni Orientalis / 中国药房

OBJECTIVE:To provide references for the quality assessment of traditional Chinese liquid medicine-Fructus Polygoni Orientalis.METHODS:The description and the physicochemical identification of Fructus Polygoni Orientalis were carried out;the contents of the extractives were determined and the chemical composition of which was studied.The quality of commercial Fructus Polygoni Orientalis medicinal substances from15nationwide areas was investigated.RESULTS:The results showed that the content of the preparation was higher than that of the raw material in the water soluble extractive assaying;the content of the alcohol-soluble extractive was the opposite.Meletin was found to be contained in the Fructus Polygoni Orien?talis acetic ether extraction liquid by the TLC identification.There was only half maturing rate for most of the commercial Fructus Polygoni Orientalis.CONCLUSION:This method can be used as a reference for the quality assessment of Fructus Polygoni Orientalis.