RESUMO
Background: Red marrow has been described as the main source of mesenchymal stem cells although its aspiration and isolation from bone marrow was reported to have significant donor site morbidity. Since secondary bone healing occurs through formation of callus as the result of proliferation and differentiation of mesenchymal stem cells, callus may become alternative source for mesenchymal stem cells. In this study, we compared the number of plastic-adherent cells from fracture site callus and bone marrow of iliac crest after two and four weeks of culture. Methods: Sixteen New Zealand rabbits were fracturized at the femoral shaft. Then, these rabbits were taken care. After two weeks of fracturization, 3 mL iliac crest bone marrow aspiration and callus extraction of eight rabbits were cultured (group I). The other eight rabbits were treated equally after four weeks of fracturization (group II). Simultaneously, the cultures were observed after one and two weeks. Four weeks later, they were harvested. Cells were counted using Neubauer hemocytometer. The average number of cells between the sources and groups were statistically analyzed using the unpaired t-test. Results: In group I, there were 2.6 ± 0.1 x 104 cells in the culture of iliac crest bone marrow aspirate and 2.5 ± 0.1 x 104 cells in culture of callus extract from fracture site (p = 0.34). In group II, there were 2.7 ± 0.1 x 104 cells and 2.1 ± 0.1 x 104 cells, respectively (p < 0.001). Conclusion: Fracture site callus at the second week post-fracturization may be potential as source of plastic-adherent cells compared with iliac crest bone marrow.
Assuntos
Células-TroncoRESUMO
OBJETIVO: Testar o efeito das c élulas tronco mesenquimais (CTM) de tecido adiposo no preenchimento cutâneo de rítides na região naso-labial. MÉTODOS: Foram coletados 50 cc de gordura da região infra-umbilical e 20 mL de sangue periférico de 15 voluntárias do sexo feminino para obtenção das CTM e de plasma autólogo, respectivamente. As voluntárias foram agrupadas de acordo com as estratégias de injeções intra-dérmicas: grupo (1) somente o ácido hialurônico; grupo (2) somente as CTM; grupo (3) CTM associadas ao ácido hialurônico. Tratando-se de um estudo prospectivo e qualitativo o acompanhamento das voluntárias era mensal através de fotografias. RESULTADOS: No grupo (1) foi observado um efeito de preenchimento imediato ao contrário do grupo (2) onde o efeito de preenchimento pleno foi alcançado aproximadamente após dois meses. No grupo (3) o preenchimento ocorreu de maneira mais efetiva e também progressiva, devido à combinação dos efeitos de curto e de longo prazo gerados pelo ácido hialurônico e pelas CTM, respectivamente. CONCLUSÃO: As CTM quando associadas ao ácido hialurônico foram capazes de promover o preenchimento de sulcos profundos, com melhora progressiva do tônus da pele e diminuição das linhas de expressão.
OBJECTIVE: To test the effect of mesenchymal stem cells (MSC) from adipose tissue on the dermal filling for nasolabial rhytids correction. MEHTODS: 50 cc of infraumbilical fat and 20 ml of peripheral blood were harvested to isolate MSC and autologous plasma from 15 female volunteers, respectively. The volunteers were grouped in according to the following strategies of intra-dermal injection: Group (I) only hyaluronic acid; Group (II) only MSC; Group (III) MSC combined with hyaluronic acid. For this qualitative and prospective study photographic monitoring was done monthly. RESULTS: In the group (I) we observed an immediate effect of filling; in the group (II) the effect of filling was reached after approximately two months. In the group (III) filling occurred more efficiently and progressively, probably due to the combination of the short and long-term effects generated by the hyaluronic acid and MSC, respectively. CONLCUSION: MSC when combined with hyaluronic acid were able to fill in deep folds, with progressive improvement of skin tone and decreasing lines of expression.