Long non-coding RNA potassium voltage-gated channel subfamily Q member 1 overlapping transcript 1 regulates the proliferation and osteogenic differentiation of human periodontal ligament stem cells by targeting miR-24-3p / 华西口腔医学杂志

OBJECTIVES@#This study aims to explore the effect and molecular mechanism of long non-coding RNA (lncRNA) potassium voltage-gated channel subfamily Q member 1 overlapping transcript 1 (KCNQ1OT1) on proliferation and osteogenic differentiation in human periodontal ligament stem cells (hPDLSCs).@*METHODS@#The hPDLSCs of normal periodontal tissues were isolated and cultured. The mineralized solution induced the osteoblast differentiation of hPDLSCs. The down-regulation of lncRNA KCNQ1OT1, the overexpression of anti-miR-24-3p on the proliferation and the levels of osteocalcin (OCN), osteopontin (OPN) and alkaline phosphatase (ALP) of hPDLSCs were investigated. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the levels of lncRNA KCNQ1OT1, miR-24-3p, OCN, OPN, and ALP. Methyl thiazolyl tetrazolium (MTT) method was used to detect cell viability and activity. Cell proliferation was evaluated by MTT. Western blot was used to detect protein expression. The targeted relationship between lncRNA KCNQ1OT1 and miR-24-3p was detected by double-luciferase experiment.@*RESULTS@#The expression level of lncRNA KCNQ1OT1 increased, and that of miR-24-3p decreased during the osteogenesis of hPDLSCs (@*CONCLUSIONS@#Down-regulation of lncRNA KCNQ1OT1 inhibited the proliferation and osteogenic differentiation of hPDLSCs by targeting the up-regulated expression of miR-24-3p.

TRIM11 promotes proliferation and invasion of breast cancer cell line MCF7 by targeting miR-24-3p / 基础医学与临床

Objective To investigate the effect of TRIM11(tripartite motif-containing protein 11) target regulating miR-24-3p on the proliferation and invasion of breast cancer cells,and potentiol relation between TRIM11 high ex-pression and the prognosis of breast cancer. Methods Immunohistochemical method was used to detect the expres-sion of TRIM11 in 31 cases of breast cancer and 31 cases of adjacent breast cancer normal tissues. The siRNA TRIM11 lentivirus and miR-24-3p lentivirus were transfected into human breast cancer MCF7 cell lines,observing the correlation expression of TRIM11 and miR- 24- 3p mRNA and protein by using real-time fluorescence quantitative PCR (RT-qPCR) and Western bolt,luciferase reporter experiments were used to verify that the miR-24-3p as a direct target of TRIM11,MTT and Transwell were used to investigate the cells proliferation,activity and invasion. Results The expression of TRIM11 in breast cancer tissues or MCF7 cells was significantly higher (P<0.05),and TRIM11 high expression predicts poor prognosis of breast cancer(P<0.05). siRNA TRIM11 significantly inhibited the expression of TRIM11 in MCF7 cells,moreover,which inhibited the MCF7 cells proliferation,viability and invasion (P<0.05). miR-24-3p significantly reduced 3′-UTR TRIM11 luciferase activity in wild-type (P<0.05),but no effect was found in mutant type.The expression of miR-24-3p was decreased,miR-24-3p and TRIM11 mRNA expression was negatively correlated in MCF7 cells(P<0.05),miR-24-3p inhibition protein and mRNA ex-pression of TRIM11 in MCF7 cells,and inhibit MCF7 cells proliferation,viability and invasion (P<0.05). Conclu-sions The expression of TRIM11 is up-regulated in breast cancer and cells,promote the proliferation and invasion of breast cancer cells though regulating miR-24-3p expression,TRIM11 high expression predicts poor prognosis of breast cancer,TRIM11/miR-24-3p axis is expected to become a new target for treatment of breast cancer.