Early stage mechanical adaptability and osteogenic differentiation of mouse bone marrow derived mesenchymal stem cell under micro-vibration stimulation environment / 生物医学工程学杂志

This study investigated the early mechanical adaptability and osteogenic differentiation of mouse bone marrow mesenchymal stem cells (M-BMSCs) under micro-vibration stimulation (MVS). M-BMSCs were stimulated by MVS , cell proliferation, alkaline phosphatase (ALP) activity assay, and cytoskeleton were measured, and cell apoptosis was observed by flow cytometry. Early osteoblast-associated genes, runt-related transcription factor 2 (Runx2), Collagen Ⅰ (Col-Ⅰ) and ALP, were observed by RT-PCR and the activation of extracellular regulated protein kinases 1/2 (ERK1/2) was determined by Western blotting. The results showed that MVS had no significant effect on the proliferation of M-BMSCs. The early apoptosis was induced by mechanical stimulation (for one day), but the apoptosis was decreased after cyclic stimulation for 3 days. At the same time, MVS significantly accelerated the expression of F-actin protein in cytoskeleton, the synthesis of ALP and the ERK1/2 pathway, also up-regulated the expressions of Runx2, Col-Ⅰ and ALP genes. This study indicates that MVS could regulate cellular activity, alter early adaptive structure and finally promote the early osteogenic differentiation of M-BMSCs.

Micro vibration with the hand nursing methods on the disuse syndrome patients with constipation nursing effect assessment / 中国实用护理杂志

Objective To investigate the micro vibration with the hand nursing methods for disuse syndrome patients constipation curative effect. Methods During the time of March 2016 to September, 76 patients who had long term disuse syndrome with the constipation in two Elderly Care Homes in Urumqi were randomly divided into intervention group (n=37) and control group (n=39), in the experimental group, was treated by manual micro vibration nursing method which included hand micro vibration therapy, exercise therapy, health education guidance once a day, 50 minutes each time, a total of 4 weeks of intervention, while the control group received routine rehabilitation nursing. Before the intervention, intervention after 4 weeks, follow-up period after the end of 8 weeks according to the symptoms of constipation and evaluate the stool frequency. Results Preliminary experimental group and control group patients have different degrees of constipation symptoms, with the hand micro vibration method of nursing intervention, the experimental group had not constipation of symptoms, 17(43.6%) had constipation symptoms in the control group. Compared with the incidence of two groups of constipation patients. Before the intervention, intervention after 4 weeks, follow-up period after the end of 8 weeks, the experimental group patients defecation frequency was (5.33 ± 1.47), (29.70 ± 6.49) , (43.41 ± 6.15)times, The control group patients was(5.77 ± 1.39), (13.10 ± 1.27), (21.15 ± 4.23) time, comparison of the two groups, t=-1.357, 15.275, 18.458, P=0.179, 0.000, 0.000. Conclusions Hand micro vibration nursing method has remarkable effect on relieving constipation, and the treatment effect can be maintained for a long time, no recurrence occurred and adverse reactions, however, this method also economic, convenient, less time consuming and efficient.

Clinical application of magnetic acupuncture / 全日本鍼灸学会雑誌

Magnetic acupuncture achieved by plastering is a particular type of acupuncture whose “pressure stimulation effect” deriving from a needle type minor process produces a synergetic effect due to “magnetic stimulation effect” originating in the magnetism concentrated on and discharged from the process. This magnetic acupuncture charged with low frequency is called “low frequency magnetic acupuncture.” The major factors in effective non-needle electro-stimulus anesthesia (T. N. S.) are the shape and condition of electrodes in their contact with the skin. Since the electrode utilizing a magnetic needle fully meets these conditions, “acupuncture sensation, ” which is clinically considered important, can be adequately expected. The following are the results obtained from our studies on the objective evaluation of a stimulation property and therapeutic effects of low frequency magnetic acupuncture.<br>The subjects involved in the study were 15 healthy adults and 5 patients with various symptoms at Naka Izu Rehabilitation Center. The method adopted was the measure of MV as an index of a comfortable sense of stimulation. An MV pick-up was attached to the subject's thum ball —if right-handed, attached to left thumb, if left-handed, attached to right thumb— with cellophane tape and was recorded by 13ch multi-purpose electro-encephalograph and DTR manufactured by Sanei Sokki K. K. before the results were analyzed by a signal processor.<br>Results:<br>1) In 10 healthy adults whose MV bands before and after T. N. S. of a general type were compared, a decrease of theta band and an increase of alpha band were recognized. When stimulation effects noted in 5 healthy adults given T. N. S. of a general type and low frequency magnetic acupuncture were compared in terms of MV pattern, a generally increasing trend of alpha band was observed in magnetic acupuncture electrodes.<br>2) MV patterns noted in 5 patients with various symptoms who were given low frequency magnetic acupuncture showed a decrease of theta and beta bands as well as an increase of alpha band, while MV amplitude showed a decrease depending on the patient. From the above results, the magnetic acupuncture electrode was found to have a more comfortable sense of stimulation than that of an electrode of an ordinary type.

The Effect of Acupuncture on Unidentified Syndrome / 全日本鍼灸学会雑誌

Introduction<br>We tried to prove the effectiveness of acupuncture against unidentified syndrome.<br>Method<br>Subjects were women complaining of unidentified syndrome and divided into the control group and test group. Galenicals extact was given to all of them and acupuncture was administered to the test group. The examination was a sequential medical trial in a restricted design (2α=0.05, 1-β=0.95, θ₁=0.90 and N=19). Acupuncture was applied twice a week for two weeks. We evaluated their subjective symptoms, difficulties in daily life and so on with MPI-test and MV-test.<br>Conclusion<br>Among the two groups, patients were randomly paired off into ten couples. Thus, a subjective comparison was possible. Global improvement rating by patients was effective for the symptoms of 60% of the test group, and 10% of the control group (p<0.05). As to the difficulties in daily life, it was effective for 70% of the test group, whereas only 20% of the control group. About subjective symptoms, there was a significant difference in the stiffness in shoulder (p<0.10). On MV-test, the test group of five pairs showed an increase in theta waves (p<0.05) and a decrease in beta waves (p<0.10).