RESUMO
IL-2 plays an important role in immune response regulation. Detection and quantitation of IL-2 is based on the use of an IL-2 dependent T cell (CTLL) or the IL-2 dependent proliferation of PHA-activated blasts. Both such clones are difficult to obtain and maintain. IL-2 assay on thymocyte activity was also used but it lacks of specificity because it also responses to IL-1.Christine (1984) developed a simple method for detection of IL-2. This is adopted in this work with some modification, and the result is compared with that obtained from CTLL assay.In brief, the ConA-activated(4ug/ml)proliferation of C57BL/6 mouse thymus cell suspension is completely blocked by hydrocortisone (HC 0.4ug/ml) The inhibition was restored by IL-2 but not by IL-1. the highest dilution of the IL-2, which restored the activity of the ConA-activated HC blocked thymus cells, is used as the titer of IL-2 quantitation for a given specimen. This was compared with that of IL-2 which can activate the CTLL. A close correlation between the titers obtained by these two assays. (r=0.94) However, the sensitivity of CTLL assay is slightly higher than that with HCIT(0.4IL-2 u and1.3 IL-2 u respectively).Hence, this technique for IL-2 detection is specific as to IL-1 is concerned, mocre feasible than the current CTLL assay, and relatively inexpensive.
RESUMO
After the major burn a multitude of immunologic alterations develop. Although the roles of suppressor cells and serum inhibitory factors in the mechanism of postburn immunosuppression are well known, the role of the neuroendo-crine response is still not understood. In this paper determining stress hormones and splenocyte immune functions are simultaneously focused on in deeply burned rats with 25% BSA. The changes of plasma corticosterone (B) and catecholamine are dominant within 24 h postburn(PB), and soon return to normal. The endogenous activity of splenocytes (SBT) decreases at 12 h PB. while the ability of spleno-cytes responsing to ConA in vitro (MSBT) is almost unchanged. The pathological lesions of spleen may be affected by increasing levels of plasma B. When most of the stress hormones return to normal at 72 h PB the SBT and MSBT become different. This may be influenced by many other factors rather than the neuroendocrine system. The differences between SBT and MSBT suggest thai SBT may reflect the more real condition of lymphocytes in vivo than MSBT in vitro. Following burns the roles of streess hormones actually participate in the immune regulation such as plasma B and catecholamine act in the early postburn stage.