RESUMO
Ulcerative colitis is a most common form of inflammatory bowel disease (IBD) which mainly affect colon. The treatment of ulcerative colitis depends upon severity of the diseases. The aim of the present study was to determine the effect of hydroalcoholic extract of dried fruits of Helicteres isora in dextran sulfate sodium induced ulcerative colitis in experimental wistar rats. In this study wistar rats of either sex were divided into five experimental groups, where control group recived only distilled water. Group 2 was negative control group which received 4% dextran sulfate sodium (DSS) from drinking water between 15th to 21st day. Group 3 received low dose of hydroalcholic extract of Helicteres isora (HI) at a dose 100mg/kg orally along with 4% DSS from drinking water between from drinking water between 15th to 21st day. Group 4 received high dose of hydroalcholic extract of Helicteres isora (HI) at a dose 200mg/kg orally along with 4% DSS from drinking water between from drinking water between 15th to 21st day. In group 5 sulfasalazine was used as a standard drug at a dose 100mg/kg orally along with 4% DSS from drinking water between from drinking water between 15th to 21st day. Twenty four hours after treatment animals were sacrificed and further macroscopical, biochemical, histopathological evaluation was done and all the results were compare with control at p<0.05 significant value.
RESUMO
Objective To detect the dynamic Th17 cells in a murine model of irritable bowel syn-drome ( IBS) and to study the effect of aryl hydrocarbon receptor ( Ahr) on Th17 cells activation .Methods Thirty BALB/c male mice were randomly divided into three groups including experiment group ,control group and Ahr antagonist group .A murine model of IBS was established by perfusing three nitrobenzene sulfonic acid (TNBS) into the colon of mice.Equal volume of saline was used to set up the control .The mice in Ahr antagonist group were intraperitoneally injected with 10 μg Ahr antagonist for four consecutive days .All mice were evaluated for visceral hypersensitivity and colonic mucosal inflammation .Mesenteric lymph nodes (MLNs) and peripheral blood mononuclear cells (PBMCs) were detected by flow cytometry through staining Th17 cells.The distribution of Ahr and IL-17A in colon and the number of Th17 cells activated by Ahr (Ahr and IL-17A double positive ) were detected by double immunofluorescence staining .Results ( 1 ) The percentage of Th17 cells in MLNs was significantly increased in experiment group followed by those in Ahr antagonist group and control group (P<0.05).(2)Compared with control group,the number of Th17 cells in peripheral blood samples was significantly increased in experiment group and Ahr antagonist group ( both P<0.05 ) ,but there was no difference between Ahr antagonist group and experiment group ( P=0.642 ) .( 3 ) The number of Ahr-activated Th17 cells ( Ahr+IL-17A+) was significantly increased in experiment group (10.00±1.58) as in comparison with that in control group (3.80±0.83,P<0.05),but the number was de-creased with Ahr antagonist intervention ( 5.80 ±0.83 , P<0.05 ) .Conclusion The number of activated Th17 cells was increased in MLNs and peripheral blood samples from mice with IBS .Ahr played an important role in the activation of Th17 cells in intestines.However,the number of Ahr-activated Th17 cells in intestinal mucosa and the proportion of Th 17 cells in MLNs could be down-regulated through blocking Ahr .