Militância enquanto convite ao diálogo: o caso da militância monodissidente / Activism as an invitation for the dialogue: the case of monodissident activism / La militancia como invitación al diálogo: el caso de la militancia monodisidente

Este artigo é uma produção teórica de caráter reflexivo que focaliza a relação entre pesquisa e militância a partir do construtivismo semiótico-cultural em psicologia, tendo como base o caso da militância monodissidente. A noção de monodissidência foi cunhada no percurso da militância bissexual para se referir a uma ferramenta analítica de ordem político-comunitária que contempla todas as pessoas que se atraem sexual e/ou romanticamente por mais de um gênero. São contrapostas concepções distintas de militância político-social em psicologia: de um lado, militância é entendida a partir de um autocentramento do militante, vinculado a uma rede de exclusões, negações, vedação e defesas psicológicas em relação à experiência; de outro, há uma compreensão dialógica de militância. Metodologicamente, a proposta de pesquisa se fundamenta no campo da participação observante, entendendo que o pesquisador está, primeiro, na condição de participante de certo campo sociocultural, a partir do qual passa a observar e refletir sobre fenômenos que ocorrem nele. Tomamos como ilustração a trajetória de construção da militância monodissidente do primeiro autor, trazendo tensionamentos dialógicos para a análise, postos em discussão com outras reflexões situadas sobre o tema. O conjunto de tensionamentos dialógicos emergidos nesse percurso foi mapeado e compreendido como um processo de multiplicação dialógica no encontro de self pesquisador com o self militante.(AU)

This paper is a theoretical production of reflective character that focuses on the relationship between research and activism from the semiotic-cultural constructivism in psychology, based on the case of monodissident activism. The notion of monodissent was coined during bisexual activism to refer to an analytical tool of a political-community order that includes all people who are sexually and/or romantically attracted to more than one gender. Different conceptions of political-social activism in psychology are opposed: on the one hand, activism is understood from the militant's self-centeredness, linked to a network of exclusions, denials, gatekeeping, and psychological defenses regarding experience; on the other hand, there is a dialogical understanding of activism. Methodologically, the research proposal is based on the field of observant participation, understanding that the researcher is, first, in the condition of a participant in a certain sociocultural field, from which he starts to observe and reflect on phenomena that occur there. We take as an illustration the trajectory of the construction of the monodissident activism of the first author, bringing dialogical tensions to the analysis, discussed with other reflections on the subject. The set of dialogic tensions that emerged in this path was mapped and understood as a process of dialogic multiplication in the encounter of the researcher self with the activist self.(AU)

Este artículo realiza una producción teórica y reflexiva sobre la relación entre investigación y activismo desde el constructivismo semiótico-cultural en Psicología, a partir del caso del activismo monodisidente. La noción de monodisidencia fue acuñada en el transcurso de la militancia bisexual para referirse a una herramienta analítica de orden político-comunitario que incluye a todas las personas que se sienten atraídas sexual y / o románticamente por más de un género. Se contraponen distintas concepciones de la militancia político-social en Psicología: por un lado, la militancia se entiende desde el egocentrismo del militante, vinculado a un entramado de exclusiones, negaciones, sellamientos y defensas psicológicas con relación a la experiencia; por otro, existe una comprensión dialógica de la militancia. La investigación utiliza como metodología la participación del observador, entendiendo que el investigador se encuentra, en primer lugar, en la condición de participante de determinado campo sociocultural, desde donde comienza a observar y reflexionar sobre los fenómenos que allí ocurren. Tomamos como ilustración la trayectoria de la construcción de la militancia monodisidente del primer autor, trayendo tensiones dialógicas al análisis, discutidas con otras reflexiones sobre el tema. El conjunto de tensiones dialógicas que surgieron en este camino se caracteriza y se comprende como un proceso de multiplicación dialógica en el encuentro del self investigador con el self militante.(AU)

Smart Spectrophotometric Methods for Concurrent Determination of Furosemide and Spironolactone Mixture in Their Pharmaceutical Dosage Forms

Abstract Simple, precise, accurate and specific spectrophotometric methods are progressed and validated for concurrent analysis of Furosemide (FUR) and Spironolactone (SPR) in their combined dosage form depend on spectral analysis procedures. Furosemide (FUR) in the binary mixture could be analyzed at its λmax 274 nm using its recovered zero order absorption spectrum using constant multiplication method (CM). Spironolactone (SPR) in the mixture could be analyzed at its λmax 238 nm by ratio subtraction method (RS). Concurrent determination for FUR and SPR in their mixture could be applied by amplitude modulation method (AM), absorbance subtraction method (AS) and ratio difference (RD). Linearity ranges of FUR and SPR were (2.0µg/mL-22.0 µg/mL) and (3.0µg/mL-30.0 µg/mL), respectively. Specificity of the proposed spectrophotometric methods was examined by analyzing the prepared mixtures in laboratory and was applied successfully for pharmaceutical dosage form analysis which have the cited drugs without additives contribution. The proposed spectrophotometric methods were also validated as per as the guidelines of ICH. Statistical comparison was performed between the obtained results with those from the official methods of the cited drugs, using one-way ANOVA, F-test and student t-test. The results are exhibiting insignificant difference concerning precision and accuracy

An efficient protocol for in vitro regeneration of peanut (Arachis hypogaea L ) cultivar L14

The present work aims to establish an efficient protocol for in vitro regeneration of peanut (Arachis hypogaea) cultivar L14. The study showed that de-embryonated cotyledon was a suitable explant for shoot multiplication on MS medium containing 4 mg/L BAP. The highest number of shoots per explant obtained after 4 weeks of culture was up to 6.8 shoots. Shoots in vitro were able to produce a large number of approximately 11 roots on MS medium supplemented with 0.5 mg/L NAA. These results will be very useful in establishing an in vitro regeneration protocol for peanut cultivar L14 during gene transfer in the next studies to improve their disease resistance.

Clonal bamboo production based on in vitro culture / Produção de plantas clonais de bambu a partir do cultivo in vitro

Bamboo species are an alternative for the composition of forest plantations. However, their potential has not been explored due to the hard time in producing large-scale clonal plants. Thus, the aim this work was to evaluate the in vitro establishment, bud multiplication and ex vitro rooting of Bambusa vulgaris. The first experiment tested different systemic and contact fungicide solutions, based on exposure time, during the establishment phase. Established explants were subjected to evaluation of residual fungicide effect on subcultures during the multiplication and elongation phases. The second experiment evaluated the influence of activated carbon on ex vitro survival and on adventitious rooting. Explant immersion in liquid culture medium added with 1.0 mL of fungicide for 120 hours has favored the in vitro establishment and reduced fungal contamination. On the other hand, it favored the shoot emission of shoots per explant during the multiplication phase. Both rooting induction culture medium and mini-incubator system use were effective in enabling adventitious root formation. The presence of activated carbon in the rooting induction culture medium resulted in a higher clonal plant survival rate.

As espécies de bambus são uma alternativa para a composição de plantios florestais. Entretanto, esse potencial não tem sido explorado devido à dificuldade de produção de mudas clonais em larga escala. Assim, objetivo deste trabalho foi avaliar o estabelecimento in vitro, a multiplicação e o enraizamento ex vitro de Bambusa vulgaris. No primeiro experimento foram testadas diferentes soluções de fungicida sistêmico e de contato em relação ao tempo de exposição durante a fase de estabelecimento. Os explantes estabelecidos foram avaliados quanto ao efeito residual do fungicida durante subcultivos nas fases de multiplicação e alongamento. No segundo experimento, foi avaliada a influência do carvão ativado sobre a sobrevivência e enraizamento ex vitro. Durante o estabelecimento in vitro, a imersão de explantes em meio de cultura líquido contendo alíquota de 1,0 mL de fungicida durante 120 horas favoreceu o estabelecimento e reduziu a contaminação fúngica, enquanto na fase de multiplicação, houve o favorecimento da emissão de brotos por explante. O meio de cultura de indução ao enraizamento e uso de sistema de mini-estufim foram efetivos para a formação de raízes adventícias e a presença de carvão ativado resultou em uma maior sobrevivência das mudas clonais.

Growth Pattern of Paecilomyces lilacinus in Different Eco-friendly Media

Paecilomyces lilacinus is a common saprophytic, filamentous fungus. Morphological characters of P. lilacinuswere separate mycelium, hyaline, conidia white to pink colored, and formation of phialides. The growth of P. lilacinus carried out on Sabouraud dextrose agar, coconut, molasses, and potato dextrose agar media at room temperature was better than incubator (25°C). The fungus has the capacity to colonize the rhizosphere and to grow in close association with nematodes. P. lilacinus was mass multiplied in both solid substrate for sorghum grains and liquid media for coconut water. Effect of temperature on the growth of P. lilacinus wasstudied in solid substrate (sorghum grain) and liquid media (coconut water) at different temperature, namely, 15, 20, 25, 30, and 35 ± 1°C. Number of colonies forming units in sorghum grain was found to be maximum at 30 ± 1°C followed by 35 ± 1°C. In liquid media (coconut water) also, maximum dry mycelial weight was recorded at 30 ± 1°C which was on par with 35 and 25 ± 1°C. It shows effect of temperature on the mycelial growth.

Nosocomial Infections: Awareness and Practices of Nurses Regarding its Spread in a Tertiary Care Hospital of Lahore, Pakistan

Introduction: The infections acquired in patients duringadmission in a hospital and the patients have no evidenceof infection before admission in hospital are known asnosocomial infections or hospital acquired infections. Theetiological organism may be bacterial, fungal, viral orparasitic, found in the air or on hospital items; spreading fromone person to another person. The main objective of currentstudy was to assess the knowledge and practices of nurseswith respect to the spread of hospital infections in a tertiaryhospital of Lahore.Material and methods: A cross sectional, descriptive studywas carried out in a tertiary hospital of Lahore, Pakistanduring a period of four months from June 2018 to September2018. Sample size was 120 and simple random sampling wasdone. Data analysis was done by SPSS version 21.Results: Most of the participants 115(95.8%) were female and5(4.2%) were male. It was revealed that nurses had enoughawareness about the spread of hospital infections. Out of120 participants, 39 (32.5%) were agree and 34(28.3%) werestrongly agree that they are aware of handwashing guidelines,but their practices to reduce the spread of hospital infectionwere not good since 38(31.7%) were neutral and 9(7.5%)were disagree to follow the recommended guidelines for usingalcohol based solutions or other antiseptics before and aftereach contact with patients.Conclusion: Nurses had a good knowledge regarding thespread of nosocomial infections, use of safety precautionsand use of alcohol based formulations but their practicesfor reducing the spread of hospital infections were not up tosatisfactory level.

Heterogeneity of math difficulties and its implications for interventions in multiplication skills / Heterogeneidade das dificuldades na aprendizagem da matemática e suas implicações para intervenções específicas nas habilidades de multiplicação

Abstract Math learning disability (MLD) is a heterogeneous condition characterized by severe and persistent difficulties in learning math, including difficulties in learning multiplication facts. Objective: In this article, we compared the responses of two MLD children to multiplication facts training. Methods: One of the children was a 9 year-old girl (HV) who presented mild math difficulties associated with lower accuracy of the Approximate Number System (ANS). The other was an 11 year-old boy (GA) who presented severe math difficulties related to impaired phonological processing due to developmental dyslexia. Both children underwent an intervention for multiplication, comprising conceptual instructions and retrieval practice of the times table. Results: HV's accuracy and response speed improved consistently on both training tasks, while GA's accuracy improved on the Simple Calculation Task only. Error analyses indicated that, after training, HV produced fewer errors of the type "close miss", and GA produced less omission but more operand errors. Conclusion: We argue that these differences between their responses to the training tasks were caused by differences in the mechanisms underlying their math difficulties. These results support the notion that individual specificities regarding math disabilities should be taken into account during preparation of training interventions.

Resumo O transtorno de aprendizagem da matemática (MLD) é uma condição heterogênea caracterizada por dificuldades acentuadas e persistentes na aprendizagem da matemática, incluindo déficits na aprendizagem dos fatos de multiplicação. Objetivo: No presente artigo, nos comparamos a resposta de duas crianças com MLD em uma intervenção da multiplicação. Métodos: Uma das crianças, HV, sexo feminino, 9 anos de idade, apresentava dificuldades menos acentuadas na matemática, associadas a um déficit no Sistema Numérico Aproximado (ANS). A outra criança, GA, sexo masculino, 11 anos de idade, apresentava dificuldades na matemática mais acentuadas associadas a um comprometimento no processamento fonológico devido a um quadro de Dislexia do Desenvolvimento. Ambas as crianças passaram por um programa de intervenção para a multiplicação, o qual se consistia em instruções conceituais e práticas de memorização da tabuada. Resultados: HV apresentou uma melhora consistente na acurácia e tempo de resposta nas duas medidas de desfecho, enquanto, GA apresentou uma melhora apenas na Tarefa de Cálculos Simples. Análises nos tipos de erros evidenciam que, após a intervenção, HV cometeu menos "erros de aproximação", ao passo que, GA cometeu menos erros por omissão, mas mais erros de operando. Conclusão: Nossa hipótese é de que as diferenças na resposta a intervenção dos participantes estão relacionadas a mecanismos subjacentes distintos à aprendizagem da matemática. Esses resultados reforçam a necessidade de que as especificidades nas dificuldades na matemática de cada paciente sejam levadas em consideração durante o planejamento das intervenções.

Effect of DEK targeting silence on proliferation and cell cycle of human hepatoma HepG2 cells / 新乡医学院学报

Objective To study the influence of targeted silencing of DEK on the proliferation and cell cycle of human hepatoma cell lines.Methods The human hepatoma cells line HepG2 were routinely cuhured and the cells were divided into blank control group,siRNA control group and DEK siRNA group when the cells grew to 90％ tusion.The cells in blank control group were cultured normally without any treatment;the cells in siRNA control group and DEK siRNA group were transfected with siRNA expression vector and DEK siRNA expression vector mediated by LipofectamineTM2000 liposomes,respectively.The expression of DEK mRNA in HepG2 cells was detected by real-time polymerase chain reaction;the expression of DEK and CyclinD1 protein in HepG2 cells was detected by Western blot;the proliferation of HepG2 cells was detected by methyl thiazolyl tetrazolium method,and the cell cycle was observed by flow cytometry.Results The expression of DEK mRNA in the blank control group,siRNA control group and DEK siRNA group was 0.826 ±0.052,0.776 ±0.051 and 0.420 ±0.050 respectively;the expression of DEK protein in the blank control group,siRNA control group and DEK siRNA group was 0.691 ± 0.073,0.726±0.061 and 0.311 ±0.038 respectively;the expression of CyclinDl protein in the blank control group,siRNA cuntrol group and DEK siRNA group was 0.712 ± 0.069,0.780 ± 0.074 and 0.434 ± 0.039 respectively.The expressions of DEK mRNA,DEK protein and CyclinD1 protein in DEK siRNA group were significantly lower than those in the blank control group and siRNA control group (P ＜ 0.05);there was no statistic difference in the expression of DEK mRNA,DEK protein and CyclinD1 protein between the blank control group and siRNA control group(P ＜0.05).The proliferation ability of HepG2 cells in DEK siRNA group after transfection of 24,48,72,96,120 h was significantly lower than that in the blank control group and siRNA control group(P ＜0.05);there was no statistic difference in the proliferation ability of HepG2 cells between the blank control group and siRNA control group at each time point(P ＜ 0.05).The proportion of G0 + G1 phase cells in DEK siRNA group was significantly higher than that in the blank control group and siRNA control group(P ＜ 0.05);the proportions of S phase and G2 + M phase cells in DEK siRNA group were significantly lower than those in the blank control group and siRNA control group(P ＜ 0.05);there was no statistic difference in the proportion of G0 + G1 phase,S phase and G2 + M phase cells between the blank control group and siRNA control group (P ＜ 0.05).The result of Pearson correlation analysis showed that the expression of CyclinD1 protein was positively correlated with the expression of DEK mRNA and protein(r =0.909,0.899;P ＜ 0.05).Conclusion DEK siRNA can inhibit the proliferation of HepG2 cells,and change the cell cycle distribution through down regulating the expression of DEK gene in HepG2 cells.This process may be related to the down regulation of the expression of CyclinD1.

La máquina de lo grupal: Una estética de la multiplicidad / The machine of lo grupal: the aesthetics of multiplicity

El artículo se centra en la figura de Eduardo Pavlovsky en el marco de la publicación Lo Grupal, una colección dirigida por el autor junto a Juan Carlos De Brasi y editada en la Argentina en posdictadura, entre 1983 y 1993. Se analizan, desde una perspectiva histórica y conceptual, los desarrollos del autor sobre la multiplicación dramática como modalidad de trabajo clínico en situación grupal. Se localizan distintos momentos de elaboración de este tópico y se indagan los problemas centrales, vinculados a la cuestión grupal, tratados por Pavlovsky y por otros autores de la publicación en torno de esta modalidad de trabajo, como la idea de producción textual colectiva, los modos de concebir la interpretación y la revisión del lugar del coordinador en un grupo. Se consideran, además, las principales recepciones que Pavlovsky incorporó en sus producciones, entre las que se destacan la idea de obra abierta de Umberto Eco y una serie de nociones de Gilles Deleuze y de Félix Guattari vinculadas a la concepción de lo inconsciente como producción.

The article is centred in the figure of Eduardo Pavlovsky within the framework of the publication Lo Grupal, a collection directed by the author together with Juan Carlos De Brasi and edited in Argentina post dictatorship, between 1983 and 1993. From a historical and conceptual perspective, the writer's developments about the dramatic multiplication as a method of clinical work in group situation are analyzed. Different moments in the production of this topic are pinpointed and the main problems linked to the issue of lo grupal are enquired into, treated by Pavlovsky and other writers according to this work method, such as the idea of collective textual production, the ways of conceiving the interpretation and the revision of the coordinator's place in a group. The main receptions Pavlovsky added in his productions among which stands out the idea of Umberto Eco's open work and a series of notions of Gilles Deleuze and Félix Guattari linked to the conception of the unconscious as production.

El carbón activado y las condiciones de oscuridad en la micropropagación de banana variedad Nanicão / Activated Carbon and dark conditions in banana micropropagation Variety Nanicão

El objetivo del trabajo fue evaluar los efectos del carbón activado y las condiciones de oscuridad inicial sobre la propagación in vitro de banana (Musa spp.) variedad Nanicão. Fueron empleados 64 ápices provenientes de hijuelos de 20 - 30 cm. Los tratamientos consistieron en la combinación de dos concentraciones de carbón activado (2 y 3 g/l) y dos condiciones de oscuridad inicial (15 y 30 días). El diseño experimental utilizado fue completamente al azar con arreglo factorial 2 x 2 y cuatro repeticiones. Cada unidad experimental estuvo constituida por cuatro ápices. Las variables evaluadas fueron la tasa media de multiplicación, el porcentaje de supervivencia, oxidación, contaminación y aclimatización de plántulas. El análisis estadístico consistió en el ANAVA con un nivel de significancia del 5 %. Las medias fueron comparadas a través del test de Tukey al 5 % de probabilidad. Los resultados mostraron que la combinación de 2 g/l de carbón activado y 15 días de oscuridad inicial promovió el mayor porcentaje de supervivencia del primer subcultivo que alcanzo 57,30 %. En la fase de establecimiento el porcentaje de contaminación de los ápices de 39,06 % mientras que en el primer subcultivo de 22,39 %. La oxidación en la fase de establecimiento fue de 9,37 % y en el primer subcultivo fue de 8,85 %. Se concluye que la combinación entre la concentración de 2 g/l de carbón activado y 15 días de período de incubación inicial resulta efectiva en el aumento de la supervivencia de los ápices.

The objective of this work was to evaluate the effects of activated charcoal and the initial darkness conditions in the propagation in vitro of banana (Musa spp.) variety Nanicão. Were employed 64 apexes coming from shoots of approximately 20 to 30 cm. The treatments consisted in the combination of two concentrations of activated charcoal (2 and 3 g/l) and two initial dark conditions (15 and 30 days). The experimental design went completely randomized with 2 x 2 factorial arrangement and four replications. Each experimental unit consisted of four apexes. Statistical analysis consisted ANOVA with significance level of 5 %. The variables were the average rate of multiplication, survival percentage, oxidation, contamination and acclimatization of plantlets. Means were compared by Tukey test at 5 % of probability. The results showed significant difference in the interaction between the concentration of 2 g/l of activated charcoal and 15 days of initial darkness at the survival percentage from the first subculture which reached 57.30 %. In the establishment phase the percentage of contamination of the apexes was 39.06 % while that in the first subculture was 22.39 %. The oxidation in the establishment phase was 9.37 % and in the first subculture was 8.85 %. It is concluded the interaction between the concentration of 2 g/l of activated charcoal and 15 days of initial incubation period is effective in increasing survival.

A minimal cost micropropagation protocol for Dianthus caryophyllus L.— a commercially significant venture.

In tissue culture, high production cost of the products restricts their reach. Though tissue culture is a major strength in floriculture it is marred by pricing issues. Hence, we developed a complete regeneration low cost micropropagation protocol for an economically important floriculture crop, carnation (Dianthus caryophyllus L.). Successful regeneration of carnation from nodal explants on cost-efficient medium indicates that psyllium husk, sugar and RO water can effectively replace the conventional medium comprising agar, sucrose and distilled water. The protocol can contribute to increased carnation production at comparatively reduced cost, and there by encourage wide scale adoption by the common growers.

Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm

The aim of this study was to establish an efficient protocol for the in vitro propagation of the endangered, endemic and decorative species Dianthus pinifolius Sibth. et Sm. The effects of different concentrations of 6-benzylaminopurine (BAP) and naphtalenacetic acid (NAA), and different explant types (single-node cuttings, terminal buds and shoot cuttings) on shoot multiplication were examined on Murashige and Skoog (MS) and half-strength MS media. The best results were obtained for shoot cuttings on the MS medium supplemented with 0.5 mg/L BAP and 0.1 mg/L NAA, achieving a maximum rate of regeneration (100%) and a total of 15.4 newly-developed shoots per explant. The highest rooting rate (96.7%) was obtained on MS medium containing 1 mg/L NAA, while the acclimatization of the microplants obtained to ex vitro conditions was successful (88.9%).

Influence of Benzyladenine and Thidiazuron on Shoot Regeneration from Leaf and Shoot tip Explants of Sedum sarmentosum Bunge

Effects of N6-benzyladenine (BA) or thidiazuron (TDZ) on adventitious shoot regeneration and axillary shoot multiplication of Sedum sarmentosum was investigated. Leaf and shoot tip explants obtained from in vitro-grown shoots of S. sarmentosum were cultured on Murashige and Skoog (MS) medium supplemented with 0, 2.0, 4.0 or 8.0 µM BA or TDZ. Of the two cytokinins studied, BA was found to be more responsive as compared to TDZ with respect to number of shoots produced per explant. High frequency of shoot regeneration (92.2%) with a mean of 12.3 shoots was produced when the leaf explants were cultured on MS medium supplemented with 8.0 µM BA. The highest number of shoots (25.4) was obtained when shoot tip explants were cultured on MS medium devoid of cytokinins after 35 days of culture. For root induction, regenerated shoots were cultured on MS medium supplemented with 0, 2.0, 4.0 or 8.0 µM indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or α-naphthaleneacetic acid (NAA). The highest number of (17.6) roots per shoot was obtained on MS medium supplemented with 2.0 µM IBA after 30 days of culture. Regenerated plantlets were successfully acclimatized in the greenhouse with 100% survival rate.

Brain Activation in Simple or Complex Multiplication Tasks in Normal Subjects:A Functional Magnetic Reso-nance Imaging Study / 中国康复理论与实践

Objective To explore the characteristics of brain activation when solving simple multiplication and complex multiplication tasks. Methods From June, 2010 to June, 2012, Thirteen normal subjects completed four functional magnetic resonance imaging (fMRI) ex-periments, including control tasks, visuospatial memory tasks, simple (single-digit) multiplication tasks and complex (multi-digit) multiplica-tion tasks. Results Compared with the control tasks, visuospatial memory tasks activated the bilateral occipital lobe, the right precuneus and superior parietal lobe;simple multiplication tasks activated the bilateral middle occipital gyri, the left superior parietal lobe, the left cingu-late gyrus, the left middle frontal gyrus and inferior frontal gyrus;complex multiplication tasks activated the right superior parietal lobe, the right inferior frontal gyrus, and the bilateral middle frontal gyri. Conclusion A right parieto-frontal network is involved in the multi-digit multiplication, which supports the containing of the spatial layout information.

Mechanism of Japanese encephalitis virus genotypes replacement based on human, porcine and mosquito-originated cell lines model

Objective: To examine the multiplication efficiency Japanese encephalitis virus (JEV) genotype I (GI) and genotype III (GIII) of different cell lines which originated from human, porcine, mosquitoes in order to prove mechanism of JEV GI replacement JEV GIII since it emerging in nature recent decades. Methods: The mixture of GI and GIII JEV isolates was inoculated on human rhabdomyosarcoma (RD), pig kidney epithelial (PS) and Aedes albopictus C6/36 clone (C6/36) which originated from human, porcine and mosquitoes, respectively. Plaque assays were performed to calculate virus titer and real-time RT-PCR with GI and GIII specific primer sets to quantify the number of GI and GIII RNA copies. Results: The highest virus titer reached at the 3rd day of post infection when GI and GIII mixture was inoculated on RD and PS and that of C6/36 was at the 4th day. JEVs were amplified and maintained by C6/36 cells after 10 passages whereas that by RD and PS only limited within 8 and 6 passages, respectively. GI strain amplified and maintained more efficiently on C6/36 and PS but not RD, whereas GIII strain amplified and maintained more efficiently on RD. Conclusions: There is a correlation between the multiplication efficiency of GI and GIII JEV strains when these two genotype strains co-infected on different cell lines with the predominance of GI strains in C6/36 and PS and the limited detection of GI strains in RD cells proving a possible mechanism of shift JEV genotypes in nature recent decades since GI emerging.

Mechanism of Japanese encephalitis virus genotypes replacement based on human, porcine and mosquito-originated cell lines model

OBJECTIVE@#To examine the multiplication efficiency Japanese encephalitis virus (JEV) genotype I (GI) and genotype III (GIII) of different cell lines which originated from human, porcine, mosquitoes in order to prove mechanism of JEV GI replacement JEV GIII since it emerging in nature recent decades.@*METHODS@#The mixture of GI and GIII JEV isolates was inoculated on human rhabdomyosarcoma (RD), pig kidney epithelial (PS) and Aedes albopictus C6/36 clone (C6/36) which originated from human, porcine and mosquitoes, respectively. Plaque assays were performed to calculate virus titer and real-time RT-PCR with GI and GIII specific primer sets to quantify the number of GI and GIII RNA copies.@*RESULTS@#The highest virus titer reached at the 3rd day of post infection when GI and GIII mixture was inoculated on RD and PS and that of C6/36 was at the 4th day. JEVs were amplified and maintained by C6/36 cells after 10 passages whereas that by RD and PS only limited within 8 and 6 passages, respectively. GI strain amplified and maintained more efficiently on C6/36 and PS but not RD, whereas GIII strain amplified and maintained more efficiently on RD.@*CONCLUSIONS@#There is a correlation between the multiplication efficiency of GI and GIII JEV strains when these two genotype strains co-infected on different cell lines with the predominance of GI strains in C6/36 and PS and the limited detection of GI strains in RD cells proving a possible mechanism of shift JEV genotypes in nature recent decades since GI emerging.

Fitorreguladores e espectros de luz na micropropagação de Oncidium baueri Lindl / Phytoregulators and light spectrum in micropropagation Oncidium baueri Lindl

Considerando que algumas espécies de orquídeas estão ameaçadas de extinção, a micropropagação é uma alternativa para a produção de um grande número de mudas com qualidade, em curto espaço de tempo. Dentre os fatores que poderão interferir na eficácia dessa técnica, podem ser citados os fitorreguladores e o espectro da luz. Assim, o objetivo do trabalho foi verificar a multiplicação e o enraizamento in vitro da orquídea Oncidium bauericom uso de diferentes concentrações de fitorreguladores e distintos filtros modificadores da luz natural. Para a multiplicação dos explantes, foram avaliados dois fatores: diferentes concentrações de 6-benzilaminopurina (BAP) (0, 1,0 e 2,0mg L-1) e espectro de luz, com uso ou não de filtros (azul, vermelho, verde); no período de enraizamento, testaram-se duas concentrações de ácido indolbutírico (AIB) (0 e 0,1mg L-1) e os mesmos níveis de luz. Concluiu-se que, durante a multiplicação, a não utilização de BAP e filtros proporciona maior número de folhas, brotações e maior comprimento dessas brotações. Na etapa de enraizamento, obtiveram-se raízes mais desenvolvidas em meio de cultura com 0,1mg L-1de AIB.

Judging from the fact that a few species of orchids are threatened with extinction, the micropropagation is an alternative to produce a bigger number of seedlings with high quality, in a shorter period of time. Among the factors that could interfere in the efficiency of this technique, some are the phytoregulators and the quality of the light. Thus, the objective of the research was to verify the multiplication and the rooting in vitro of the orchid Oncidium baueriusing different concentrations of phytoregulators and distinct modifiers filters of natural light. Concerning the multiplication of the explants, two factors have been evaluated: different concentrations of 6-benzylaminopurine (BAP) (0, 1,0 and 2,0mg L-1) and light spectrum, with or without filters (blue, red, green); and in the period of rooting, two concentrations of indolebutyric acid (IBA) (0 and 0,1mg L-1) and the same levels of light have been tested. It's possible to conclude that during the multiplication, not using BAP and filters provides a bigger number of leaves, more sprouting and bigger length of these sprouts. In the rooting phase, more developed roots were obtained in 0,1mg L-1 de AIB culture.

A simple cost effective method for mass propagation of Chrysanthemum morifolium and antibacterial activity assessment of in vitro raised plantlets.

An efficient low cost procedure for in vitro propagation of Chrysanthemum morifolium has been developed with subsequent assessment of antibacterial property of in vitro raised plantlets. Optimal axillary shoot multiplication was achieved on MS medium supplemented with low concentration of BAP. Psyillium husk and market sugar were standardized as suitable alternatives to the conventionally used agar and sucrose, cutting down the production cost of tissue culture raised plantlets to over six times. Optimal in vitro rooting was obtained on half strength MS medium containing IBA. Regenerated plantlets with well developed shoots and roots were acclimatized successfully and transferred to field conditions where they flowered. The leaves of ex vitro growing tissue culture raised plantlets were later assessed for activity against bacterial pathogens. The present protocol ensures minimal cost input in large scale production of a commercially important ornamental plant and opens up scope of scientific interventions directed at its allied therapeutic usage. Abbreviations: MS: Murashige and Skoog (1962); HgCl2 : Mercuric chloride; PGR: Plant growth regulator, TCR: tissue culture raised; BAP: 6, Benzylaminopurine; NAA: α-Naphthalene Acetic Acid; IBA: Indole-3 butyric acid; IAA: Indole-3 acetic acid; min: minutes; ***:significant at 99.9%.

Photographic Estimation of Intercanthal Width of Bangladeshi Women.

Background: Estimation of intercanthal width and biocular breadth from digital photography is important in the evaluation of several systemic syndromes, craniofacial abnormalities and in surgical treatments of post-traumatic telecanthus. Dysmorphologists employ canthal measurements in evaluating the degree of hypertelorism. So intercanthal width and biocular breadth values are very important for clinicians, ophthalmologists, plastic and reconstructive surgeons. The reliability of estimation of intercanthal width from biocular breadth by using multiplication factor is high. Objective: To make an attempt to determine the multiplication factor for estimation of intercanthal width. Materials and method: This is a cross sectional study conducted in the Anatomy department of Sir Salimullah Medical College, Dhaka, Bangladesh from January 2010 to June 2011. One hundred Bangladeshi 25 to 45 years old women were selected purposively for the study. Results: The mean±SD intercanthal width was 3.456±0.3585 cm. Multiplication factor for measuring intercanthal width from biocular breadth (9.348±0.7174 cm) was 0.369±0.0232 cm. The mean±SD of estimated intercanthal width was 3.449±0.2647 cm. No significant difference was found between the measured and estimated intercanthal width. Conclusion: Multiplication factor can be used to determine intercanthal width in Bangladeshi women though agreement test would have been done to generalize the finding.

Studies on Influence of Growth Regulators in Micropropagation of Lavandula Angustifolia.

Lavandula angustifolia (Family Labiates) is a medicinal herb found in Mediterranean area. It is a well known herb in ayurvedic system of medicines and has traditionally been used to treat disorder of liver, fever and several conditions including infertility, infection and anxiety. There are few reports on tissue culture of Lavandula angustifolia that too mainly on micropropagation. Present study explored an in vitro micropropagation of Lavandula angustifolia. In vitro callus formation was established by using nodal segments on Murashique and Skoog, (1962) medium (MS) supplemented with IAA at 0.1mg/l, l BAP at 0.002mg/l and 2-4D at 0.2mg/l, significantly recorded complete callus formation after 6 weeks of incubation at 25±1ºC. The callus was allowed for organogenesis and then shoot multiplication was carried out at 4 concentrations of BAP (0.5, 1, 2 and 1mg/L) and IAA (0.5, 0.5, 0.5 and 1mg/L) on MS medium. The shoot regeneration medium for shoot multiplication and proliferation with higher number of shoots was recorded at 0.5mg/l of IAA and 2.0mg/l BAP. However, the growth was very steady and originating from the base. MS medium without any growth regulators tabulated the tallest shoot length of 35 mm, but the shoots were clustered not properly differentiated.