RESUMO
Objective: To study the inhibitory effect of the medicine group of promoting blood circulation and removing stasis (PBCRS) on breast cancer induced by 7, 12-dimethylbenz(a)anthracene (DMBA) in rats, and screen out and verify key genes based on RNA Sequencing (RNA-seq) technology and Ingenuity Pathway Analysis (IPA). Method: Totally 96 Sprague-Dawley (SD) rats were randomly divided into blank group, DMBA model control group, tamoxifen (TAM) group (1.9 mg·kg-1·d-1), high-dose, middle-dose and low-dose PBCRS groups (12.96, 6.48, 3.24 g·kg-1·d-1). One week after drug intervention, except for the blank group, the DMBA was used to induce the rat model of breast cancer (with an interval of a week, irrigation for two times at the dose of 100 mg·kg-1). After 10 weeks, the changes in tumor weight and tumor volume were observed. The total RNA was extracted by total RNA extraction kit, and three RNA samples were collected from the DMBA model control group and the middle-dose PBCRS group for genetic testing. Based on RNA-seq, key differential genes were screened out and verified by Real-time PCR. Result: Comparing with the DMBA model control group, the tumor volume and tumor weight in middle-dose PBCRS group were decreased significantly (PPConclusion: PBCRS may inhibit the occurrence of breast cancer by interfering with the expression of FBP1 in breast cancer tissue.
RESUMO
Objective:To study the degradation regularity of Moglobin (Mb) mRNA in rats and to explore the relationship between the time of death.Methods:Using RT-PCR to detect the Mb mRNA levels of the corresponding time changes in rat heart after death at 25℃?1℃, 65%～70%temperature and humidity control system,and use SPSS 17.0 software for data analysis.Results:The contents of the Mb mRNA in rats gradually decreased with the lengthening of the postmortem interval,and it had a proximate linear relationship with the postmortem interva1.The degradation of Mb mRNA is lower than that in vitro.Conclusion:Mb mRNA was time-related degradation,and the degradation under the conditions of the law was not entirely consistent in vivo and vitro;Mb could be used for estimating the earlier postmortem interva1.