Research Progress on Estimation of Postmortem Interval Based on Ocular Tissues Structure / 法医学杂志

Estimation of postmortem interval (PMI) is one of the important research contents in forensic pathology, and it has always been the focus and hot spot of research work. In recent years, scholars at home and abroad have made some research progress in estimating PMI by using ocular tissue. After death, the changes of cornea, aqueous humor, iris, lens, vitreous humor and retina all show time sequence change rule highly related to PMI. This paper reviews the research progress of PMI estimation based on the morphological, biochemical, molecular and genetic material changes of different ocular tissue structures after death, and discusses the existing problems and development trends.

Estimation of postmortem interval by ocular tissues / 中国法医学杂志

Estimation of postmortem interval(PMI) by ocular tissues has been a new top subject in the forensic research and practice ifelds. A lot of research results showed that the changes of cornea, retina, vitreous humor and aqueous humor and so on exist a high linear correlation with PMI, which can be used to estimate PMI. This paper reviewed recent progress of study on them.

Immunohistochemical Localization of Transglutaminase 4 in the Human Eye

PURPOSE: Transglutaminase 4 (TGase 4) belongs to a family of enzymes that catalyzes the post-translational modification of proteins. In an attempt to establish its physiological function(s), the distribution of TGase 4 expression in the human eye was determined. METHODS: Ocular tissues obtained from five human whole eyeball postmortem (40(+1) weeks at gestation age, 2 months, 48, 66, 76 years) were stained with monoclonal antibodies against human TGase 4 using indirect immunohistochemical method. RESULTS: TGase 4 was found in the lacrimal glands, corneal epithelium and endothelium, conjunctival epithelium, lens epithelium, retina (inner segment of photoreceptor, external limiting membrane, outer plexiform layer, inner plexiform layer, retinal nerve fiber layer and internal limiting membrane), iris, ciliary muscle, ciliary nonpigmented epithelium and trabecular meshwork. Endothelium of blood vessels in all ocular tissues was also stained. Conjunctival stroma, choroid, anterior tenon's capsule were faintly stained. No evidence of immunostaining for TGase 4 was found in the corneal stroma, iris stroma, lens nucleus, ciliary process, sclera, extraocular muscle and optic nerve. CONCLUSIONS: The expression pattern of TGase 4 was different from those of other TGase isoforms in the human eye. This result may be helpful in further investigation of the role of TGase 4 in the ocular tissue.

Expression of AQP1 in ocular tissues of rat / 重庆医科大学学报

Objective:To detect the expression of aquaporin-1(AQP1) in ocular tissues of normal rats.Methods:The eyeballs were enucleated from the male Wistar rats,fixed with 4% formaldehydum polymerisatum and embeded in paraffin.Then,5?m serial section was made on the sagittal plane.Each sample was cut into 3 pieces for AQP1 HE staining and immunohistochemical staining,the latter of which included negative control and positive control.The immunohistochemical staining of renal tubule,known as positive expression of AQP1,was used as positive control.Results:Under the light microscope,the brown cellular granules indicated positive-AQP1 cells.AQP1 was expressed in corneal endothelial cells,iris epithelial cells,crystalline lens epithelial cells,ciliary body non-pigment epithelial(NPE) cells,ganglion cell layer and inner nuclear layer of retina,optic nerve glial cells.Conclusions: AQP1 is expressed in many places of ocular tissues related to the water metabolism.