Study on loss of OprD_2 in imipenem-resistant Pseudomonas aeruginosa / 临床检验杂志

Objective To investigate the loss of outer membrane protein D2 (OprD2) gene in imipenem-resistant Pseudomonas aeruginosa.Methods The minimal inhibitory concentrations (MIC) of 10 antimicrobial agents against 80 strains of imipenem-resistant clinical isolates were determined by agar dilution method.PCR was used to detect OprD2 gene.Results PCR results showed that 44 of the 80 isolates of imipenem-resistant P.aeruginosa were positive for OprD2 gene.There was significant difference (P

ERIC-PCR Fingerprinting Related with oprD_2 Gene of Pseudomonas aeruginosa Isolates Resistant to Imipenem / 中华医院感染学杂志

0.05).Thirty genotypes were got from 54 strains by ERIC-PCR.CONCLUSIONS There isn′t significant difference on the absence rate of oprD2 gene in Tianjin and it gives us a hint that other factors can also result in the prevalence of IRPA.The existence of prevalence of IRPA clone in some departments advises us to effectively control the IRPA in hospital.