Effects of Rehmannia and Storesin on Nitric Oxide, Nitric Oxide Synthase and Tumor Necrosis Factor α in Early Hepatic Encephalopathy Rat Model / 中国康复理论与实践

@# Objective To investigate the effects of Rehmannia and Storesin (RS) on early hepatic encephalopathy (HE) in rat model.Methods HE rat model was induced by CCl4 intragastric administration. The effects of RS on serum nitric oxide (NO) and nitric oxide synthase(NOS) as well as hippocampal tumor necrosis factor α (TNF-α) level of animals were evaluated in 3 dose groups. Lactulose was usedas the positive group. Results The serum NO and NOS as well as hippocampal TNF-α level of the model rats were significantly increasedcompared with that in control animals (P<0.01). The RS high dosage treatment could significantly decrease the levels of those indexes (P<0.01). Conclusion Rehmannia and Storesin is effective on early HE by decreasing the level of serum NO and NOS as well as hippocampalTNF-α.

Effect of Pretreatment of Aprotinin on Nitric Oxide and Nitric Oxide Synthase Contents after Spinal Cord Ischemia-reperfusion Injury in Rabbits / 中国康复理论与实践

@#Objective To observe the effect of pretreatment of aprotinin on nitric oxide (NO) and nitric oxide synthase (NOS) contents after ischemia-reperfusion injury of spinal cord in rabbits.Methods 45 rabbits were randomly divided into aprotinin treatment group (group A), normal saline control group (group B) and pseudo-surgical operation group (group C) with 15 rabbits in each group. The infrarenal segment in abdominal aorta was clamped for 60 min to construct the model of lumbosacral spinal cord ischemia in rabbits. Reperfusion was followed and kept on for 24 h until the blood flow regained normal. Aprotinin was given 3×107 IU/kg as a short time intravenous injection for 10 min before ischemia, and then was drilled with micro pump by 1×107 IU/kg/h. Normal saline was used in group B, the ischemia-reperfusion duration between group A and group B remained same. The group C was only exposured abdominal aorta and not clamped. The rabbits were killed before ischemia and at 8 h, 24 h after ischemia-reperfusion, lumbar segment spinal cords were harvested to detect contents of NO and NOS of spinal cord.Results After 8 h of ischemia-reperfusion,the contents of NO, total NOS (TNOS), and induced NOS (iNOS) in group A and group B were more than that before ischemia (P＜0.05). After 8 h of ischemia-reperfusion, there was a significant difference in the contents of NO, TNOS, iNOS between group A and group B (P＜0.05～0.01). After 24 h of ischemia-reperfusion, there was a significant difference too between group A and group B (P＜0.01). After 8 h and 24 h ischemia-reperfusion, the contents of NO, TNOS, iNOS in group A and group B were more than that in group C (P＜0.01).Conclusion During the ischemia-reperfusion, more NO produced is an important factor of spinal cord injury. Aprotinin can decrease the contents of NO and ischemia-reperfusion injury to spinal cord of rabbits.

Effect of vesnarinone on PKC activity and NO content on myocardial injury induced by ischemia and reperfusion in rats / 中国药理学通报

AIM To study the protective effects of vesnarinone on myocardial ischemia. METHODS Rat myocardial oxide nitric (NO) content, protein kinase C (PKC) activities in different groups were determined by kits. RESULTS Vesnarinone 1 mg?kg -1 or 2 mg?kg -1 iv markedly improved the NO content and PKC activity before ischemia for 30 min and reperfusion for 2 h in rats. CONCLUSION Vesnarinone possesses a protective effect against myocardial ischemia via NO induce PKC.