Effects of shRNA targeting PRL-3 on proliferation,invasion and apoptosis of human colorectal cancer cells / 天津医药

Objective To investigate the effects of PRL-3 shRNA mediated by lentivirus on proliferation, invasion and apoptosis of human colorectal cancer SW480 cells. Methods There were three experimental groups in this study, which included blank control group, negative control group and transfected group. Colorectal cancer SW480 cells were transfected with lentiviral interference vector carrying PRL-3 shRNA to build a stable PRL-3-silencing cell line. The expression of PRL-3 mRNA was detected by real-time quantitative polymerase chain reaction (real-time PCR). Cell proliferation was analyzed by MTT method and colony formation assay.Invasion and migration were measured by Transwell assay and invasion chamber. Apoptosis rate was performed by flow cytometry. Results The stable PRL-3-silencing cell line was successfully constructed.Compared with the blank control group and negative control group,the relative expression levels of PRL-3 mRNA were reduced in transfected group after transfection with PRL-3 shRNA(P<0.05),but there was no significant difference between the blank control group and the negative control group.After transfection with PRL-3 shRNA for 72 h,the proliferation of SW480 cells was significantly lower in transfected group than that of the blank control group and the negative control group,and the proliferation decreased significantly in 120 h(P<0.05).Compared with the blank control group and negative control group, the ability of colony formation was also weakened in the transfected group (P<0.05). Compared with the blank control group and negative control group,the migration and invasion ability were decreased in the transfected group(P<0.05),and the apoptosis rate was increased in the transfected group(P<0.05).Conclusion PRL-3 shRNA can inhibit the expression of PRL-3 and the proliferation, promote the apoptosis of SW480, which indicates that PRL-3 may become a target for colorectal carcinoma treatment.

Expression and relationship of PRL-3,tumor associated macrophags and lymphangiogenesis in papillary thyroid carcinoma / 实用医学杂志

Objective To investigate the relationship of PRL-3,tumor associated macrophages and lym-phangiogenesis in papillary thyroid carcinoma. Methods SP immunohistochemistry was used to study the levels of PRL-3,CD68,and D2-40 in papillary thyroid carcinoma and thyroid adenoma.Results The positive expression rates of PRL-3,CD-68 and D2-40 were higher in papillary thyroid carcinoma than those in thyroid adenoma (P < 0.01). High PRL-3,CD-68 or D2-40 was associated with lymphatic metastasis in patients with papillary thyroid carcinoma(P<0.01).Conclusion The expression levels of PRL-3,CD-68 and D2-40 are positively cor-related in papillary thyroid carcinoma,and they are related to invasion and lymphangiogenesis of papillary thyroid carcinoma.

Advances of phosphatase of regenerating liver-3 in hematological malignancies / 中国病理生理杂志

In recent years,accumulating evidence shows that phosphatase of regenerating liver-3(PRL-3)is closely implicated in tumor progression,especially in the stages of invasion and metastasis of various solid tumors, inclu-ding colorectal cancer,gastric cancer and breast cancer.However,the study of PRL-3 in hematological malignancies is rel-atively lagged,but there are some advances in leukemia and myeloma,in which PRL-3 up-regulation is tightly associated with poor prognosis,while the underlying mechanism of signal transduction is gradually explored.In this review,we sum-marized the recent advances of PRL-3 in hematological malignancies such as acute myeloid leukemia,multiple myeloma and chronic myeloid leukemia,as well as the molecular mechanism of PRL-3 in pathogenesis.

Study of signaling pathway related tophosphatase of regenerating liver 3 / 医学研究生学报

PRL-3, belonging to the family of protein tyrosine phosphatase, has been found to be high expressed in colorectal cancer, gastric cancer, ovarian cancer and melanoma.It also has been reported that PRL-3 plays an important role in the progression of tumor.With the distinction of PRL-3 in tumor progression, especially in the metastatic tumor, there are more and more researches about the regulating mechanism of PRL-3.This paper reviews the development of signaling pathway of PRL-3.

Do phosphatase of regenerating liver‑3, matrix metalloproteinases‑2, matrix metalloproteinases‑9, and epidermal growth factor receptor‑1 predict response to therapy and survival in glioblastoma multiforme.

Context: Poor survival of the glioblastoma multiforme (GBM) has been attributed in part to the invasive nature of the lesion making complete surgical removal near impossible. Phosphatase of regenerating liver‑3 (PRL‑3), matrix metalloproteinases‑2 and ‑9 (MMP‑2 and MMP‑9), and epidermal growth factor receptor (EGFR‑1) play a role in invasive nature of tumor cells. Aims: This study was conducted to evaluate PRL‑3, MMP‑2, MMP‑9, and EGFR‑1 (markers) expression in cases to GBM and to correlate their expression with therapy response and survival. Settings and Design: GBM cases (n = 62) underwent surgery followed by radiation (n = 34) and chemoradiation (n = 28). Using WHO Response Evaluation Criteria in Solid Tumors criteria response to therapy was assessed at 3 months and cases followed up for survival. Subjects and Methods: Expression of markers was assessed by immunohistochemistry as a percentage of positive tumor cells in hot spots. Statistical Analysis Used: Kaplan–Meier, ANOVA, Chi‑square test, univariate, and multivariate Cox‑regression analysis was done. Results: Response to therapy was evident in 54.8% cases of responders with the mean survival of 494.03 ± 201.13 days and 45.2% cases of non responders (278.32 ± 121.66 days) with P = 0.001. Mean survival for the patient’s opted chemoradiation was 457.43 ± 222.48 days which was approximately 3 months greater than those who opted radiation alone (P = 0.029). We found PRL‑3 overexpression was an independent, significant, poor prognostic factor for survival by multivariate analysis (P = 0.044). Cases negative for MMP’s and EGFR showed increased survival, but the difference was insignificant. Conclusion: PRL‑3 expression appears to be related to an adverse disease outcome.

Research progress of phosphatase of regenerating liver-3 in tumors / 肿瘤研究与临床

Phosphatase of regenerating liver-3 (PRL-3) is one of member in the protein tyrosine phosphatase (PTP) family.Since high expression of PRL-3 was first discovered in liver metastasis patients with colon cancer,many researchers found that PRL-3 also high expressed in multiple organs like cancer from breast,prostate,liver,stomach,lung,kidney and so on.This paper focused on correlation of PRL-3 with the occurrence and metastasis of tumors.