RESUMO
The preparation of 7 meso-tetrasubstituted phenyl porphines and the photosensitizing effects and tumor-photobiological activities of 4 of these compounds in cell-free system are presented here.It has been shown by the data in determination of the ability of compounds Ⅰ1_4 to sensitize photooxidation of NADPH in D2O under the illumination _of He-Ne laser that the ability to generate active oxygen species of them was much higher than those of YHpD and PsD-007.But they were weak in photoinactivation for the cultured human tongue squamocell cancer cells TCA-8113 in vitro.However, they were effective, in some degree, in the photo-localization and photodynamic therapy of sarcoma Si80 in mice.Severe delayed phototoxic reaction occurred in the mice administered these compounds.Porphines with halogen atoms in their molecules (compounds Ⅱ1_3) could not be excited and were non-fluorescent.This suggests that there was possibly a quenching effect of ha'ogen atoms on porphine fluorescence.
RESUMO
The chemical composition, separation and identification of the components as well as the tumor photobiological activities of the major components of the new tumor-photochemodiagnostic and photochemotherapeutic agent photocarcinorin (PsD-007) were represented in this paper. It has been shown by the results of HPLC analysis in combination with spectroscopic determinations that PsD-007 is composed of 7 different porphyrins: MHD, DMD, MVD, AHD, HVD, Hp and Pp. The experimental results show that MHD, DMD and MVD are the major tumor photobiologically active components of PsD-007.