Variations in soil physico-chemical properties, soil stocks, and soil stoichiometry under different soil layers, the major forest region Liupan Mountains of Northwest China

Abstract Liupan Mountains are an important region in China in the context of forest cover and vegetation due to huge afforestation and plantation practices, which brought changes in soil physio-chemical properties, soil stocks, and soil stoichiometries are rarely been understood. The study aims to explore the distribution of soil nutrients at 1-m soil depth in the plantation forest region. The soil samples at five depth increments (0-20, 20-40, 40-60, 60-80, and 80-100 cm) were collected and analyzed for different soil physio-chemical characteristics. The results showed a significant variation in soil bulk density (BD), soil porosity, pH, cation exchange capacity (CEC), and electric conductivity (EC) values. More soil BD (1.41 g cm-3) and pH (6.97) were noticed in the deep soil layer (80-100 cm), while the highest values of porosity (60.6%), EC (0.09 mS cm-1), and CEC (32.9 c mol kg-1) were reflected in the uppermost soil layer (0-20 cm). Similarly, the highest contents of soil organic carbon (SOC), total phosphorus (TP), available phosphorus (AP), total nitrogen (TN), and available potassium (AK) were calculated in the surface soil layer (0-20 cm). With increasing soil depth increment a decreasing trend in the SOC and other nutrient concentration were found, whereas the soil total potassium (TK) produced a negative correlation with soil layer depth. The entire results produced the distribution of SOCs and TNs (stocks) at various soil depths in forestland patterns were 020cm > 2040cm > 4060cm 6080cm 80100 cm. Furthermore, the stoichiometric ratios of C, N, and P, the C/P, and N/P ratios showed maximum values (66.49 and 5.46) in 0-20 cm and lowest values (23.78 and 1.91) in 80-100 cm soil layer depth. Though the C/N ratio was statistically similar across the whole soil profile (0-100 cm). These results highlighted that the soil depth increments might largely be attributed to fluctuations in soil physio-chemical properties, soil stocks, and soil stoichiometries. Further study is needed to draw more conclusions on nutrient dynamics, soil stocks, and soil stoichiometry in these forests.

Resumo As montanhas de Liupan são uma região importante na China no contexto de cobertura florestal e vegetação devido às enormes práticas de florestamento e plantação, que trouxeram mudanças nas propriedades físico-químicas do solo, e estoques e estequiometrias do solo raramente são compreendidos. O estudo visa explorar a distribuição de nutrientes do solo a 1 m de profundidade do solo na região da floresta plantada. As amostras de solo em cinco incrementos de profundidade (0-20, 20-40, 40-60, 60-80 e 80-100 cm) foram coletadas e analisadas para diferentes características físico-químicas do solo. Os resultados mostraram uma variação significativa nos valores de densidade do solo (BD), porosidade do solo, pH, capacidade de troca catiônica (CEC) e condutividade elétrica (CE). Mais DB do solo (1,41 g cm-3) e pH (6,97) do solo foram observados na camada profunda do solo (80-100 cm), enquanto os maiores valores de porosidade (60,6%), CE (0,09 mS cm-1) e CEC (32,9 c mol kg-1) foram refletidos na camada superior do solo (0-20 cm). Da mesma forma, os maiores teores de carbono orgânico do solo (SOC), fósforo total (TP), fósforo disponível (AP), nitrogênio total (TN) e potássio disponível (AK) foram calculados na camada superficial do solo (0-20 cm). Com o aumento do incremento da profundidade do solo, uma tendência decrescente no SOC e na concentração de outros nutrientes foi encontrada, enquanto o potássio total do solo (TK) produziu uma correlação negativa com a profundidade da camada do solo. Todos os resultados produziram a distribuição de SOCs e TNs (estoques) em várias profundidades de solo em padrões de floresta 0 20cm> 20 40cm> 40 60cm 60 80cm 80 100 cm. Além disso, as relações estequiométricas de C, N e P, as relações C / P e N / P, apresentaram valores máximos (66,49 e 5,46) em 0-20 cm, e valores mais baixos (23,78 e 1,91) em solo de 80-100 cm profundidade da camada. Embora a relação C / N fosse estatisticamente semelhante em todo o perfil do solo (0-100 cm). Esses resultados destacaram que os incrementos de profundidade do solo podem ser amplamente atribuídos a flutuações nas propriedades físico-químicas do solo, estoques e estequiometrias do solo. Mais estudos são necessários para tirar conclusões adicionais sobre a dinâmica dos nutrientes, estoques de solo e estequiometria do solo nessas florestas.

Variations in soil physico-chemical properties, soil stocks, and soil stoichiometry under different soil layers, the major forest region Liupan Mountains of Northwest China / Variações nas propriedades físico-químicas do solo, estoques e estequiometria do solo sob diferentes camadas de solo, a principal região florestal das montanhas Liupan do noroeste da China

Liupan Mountains are an important region in China in the context of forest cover and vegetation due to huge afforestation and plantation practices, which brought changes in soil physio-chemical properties, soil stocks, and soil stoichiometries are rarely been understood. The study aims to explore the distribution of soil nutrients at 1-m soil depth in the plantation forest region. The soil samples at five depth increments (0-20, 20-40, 40-60, 60-80, and 80-100 cm) were collected and analyzed for different soil physio-chemical characteristics. The results showed a significant variation in soil bulk density (BD), soil porosity, pH, cation exchange capacity (CEC), and electric conductivity (EC) values. More soil BD (1.41 g cm-3) and pH (6.97) were noticed in the deep soil layer (80-100 cm), while the highest values of porosity (60.6%), EC (0.09 mS cm-1), and CEC (32.9 c mol kg-1) were reflected in the uppermost soil layer (0-20 cm). Similarly, the highest contents of soil organic carbon (SOC), total phosphorus (TP), available phosphorus (AP), total nitrogen (TN), and available potassium (AK) were calculated in the surface soil layer (0-20 cm). With increasing soil depth increment a decreasing trend in the SOC and other nutrient concentration were found, whereas the soil total potassium (TK) produced a negative correlation with soil layer depth. The entire results produced the distribution of SOCs and TNs (stocks) at various soil depths in forestland patterns were 0→20cm > 20→40cm > 40→60cm ≥ 60→80cm ≥ 80→100 cm. Furthermore, the stoichiometric ratios of C, N, and P, the C/P, and N/P ratios showed maximum values (66.49 and 5.46) in 0-20 cm and lowest values (23.78 and 1.91) in 80-100 cm soil layer depth. Though the C/N ratio was statistically similar across the whole soil profile (0-100 cm). These results highlighted that the soil depth increments might largely be attributed to fluctuations in soil physio-chemical properties, soil stocks, and soil stoichiometries. Further study is needed to draw more conclusions on nutrient dynamics, soil stocks, and soil stoichiometry in these forests.

As montanhas de Liupan são uma região importante na China no contexto de cobertura florestal e vegetação devido às enormes práticas de florestamento e plantação, que trouxeram mudanças nas propriedades físico-químicas do solo, e estoques e estequiometrias do solo raramente são compreendidos. O estudo visa explorar a distribuição de nutrientes do solo a 1 m de profundidade do solo na região da floresta plantada. As amostras de solo em cinco incrementos de profundidade (0-20, 20-40, 40-60, 60-80 e 80-100 cm) foram coletadas e analisadas para diferentes características físico-químicas do solo. Os resultados mostraram uma variação significativa nos valores de densidade do solo (BD), porosidade do solo, pH, capacidade de troca catiônica (CEC) e condutividade elétrica (CE). Mais DB do solo (1,41 g cm-3) e pH (6,97) do solo foram observados na camada profunda do solo (80-100 cm), enquanto os maiores valores de porosidade (60,6%), CE (0,09 mS cm-1) e CEC (32,9 c mol kg-1) foram refletidos na camada superior do solo (0-20 cm). Da mesma forma, os maiores teores de carbono orgânico do solo (SOC), fósforo total (TP), fósforo disponível (AP), nitrogênio total (TN) e potássio disponível (AK) foram calculados na camada superficial do solo (0-20 cm). Com o aumento do incremento da profundidade do solo, uma tendência decrescente no SOC e na concentração de outros nutrientes foi encontrada, enquanto o potássio total do solo (TK) produziu uma correlação negativa com a profundidade da camada do solo. Todos os resultados produziram a distribuição de SOCs e TNs (estoques) em várias profundidades de solo em padrões de floresta 0 → 20cm> 20 → 40cm> 40 → 60cm ≥ 60 → 80cm ≥ 80 → 100 cm. Além disso, as relações estequiométricas de C, N e P, as relações C / P e N / P, apresentaram valores máximos (66,49 e 5,46) em 0-20 cm, e valores mais baixos (23,78 e 1,91) em solo de 80-100 cm profundidade da camada. Embora a relação C / N fosse estatisticamente semelhante em todo o perfil do solo (0-100 cm). Esses resultados destacaram que os incrementos de profundidade do solo podem ser amplamente atribuídos a flutuações nas propriedades físico-químicas do solo, estoques e estequiometrias do solo. Mais estudos são necessários para tirar conclusões adicionais sobre a dinâmica dos nutrientes, estoques de solo e estequiometria do solo nessas florestas.

Comparative Study of Physico-Chemical Analysis of Changeri Ghrita Prepared with Murchita and Amurchita Ghrita

Changeri ghrita is a medicated ghee formulation, mentioned in Grahani rog chikitsa. Sneha murchana is a procedure prior to Snehapaka. Snehapaka and Murchana bring changes in Sneha. Materials and Methods: Two batches of Changeri ghrita prepared using Murchita and Amurchit ghrita. Prepared Ghrita subjected to physico-chemical analysis. Result and Discussion: Specific gravity increased in Murchita ghrita. Refractive index, viscosity, saponification value, iodine values were increased. While acid value is decreased. Peroxide value is slightly increased. Unsaponifiable matter remains unchanged. There was change in organoleptic properties after Murchana. Physico-chemical changes have been occurring except in unsaponifiable matter and congealing point. Peroxide was present in both samples of Amurchit and Murchit Changeri ghrita. Saponification value was increased in Murchit Changeri Ghrita Conclusion: It can be concluded that antioxidants were added during Murchana. Murchana maintains stability of lipid preparation and offer good health impact, increases palatability.

ADMET, Pharmacokinetic and Docking properties of the fungal drug 2- (2, 4-difluorophenyl)-1, 3-bis (1, 2, 4-triazol-1-yl) propan-2-ol by using Quantum computational methods

The current study contributes to a better knowledge of the FCZ's characteristics and bioactivity. The ADMET properties have been calculated and the results have been illustrated; as a result, it has become quite popular for virtual pharmaceutical analysis. This research aims to examine FCZ’s optimized structure and properties by analyzing various computational calculations. Bond length, Bond angle, Mulliken charges have been analyzed for the studies. The experimental geometrical parameters and theoretical data were compared with ADME parameters, biomarker properties, pH value, drug like nature, Marvin sketch, Swiss ADME to quantify molecular descriptors just as to survey atomic elements. ADMET properties introduce the influence of the drug levels and its kinetics with the tissues of the body. It also explains about the metabolism, toxicity of the drugs when introduced to the system. The analysis on pharmacokinetic properties has helped a lot in the drug development for further studies. The target prediction of FCZ has been studied along with the docking study. Docking study is an important program in order to study about the binding of the small ligand into a receptor like proteins. This method is very useful in drug discovery which provides insights into various studies. This will help in further development of the drugs which will finally help the society in large scale. FCZ helps pharmaceutical industry in developing the drugs to treat chronic disease when combined with other molecules. Hence the present study is really helpful in drug designing and in the development of new drugs.

Simultaneous detection of thirteen exons of dystrophin gene by optimized multiplex PCR assay to screen Duchenne/Becker muscular dystrophy

Advancements in Polymerase Chain Reaction (PCR) technology and other techniques like Deoxyribonucleic acid (DNA)signal and target amplification have become key procedures in molecular diagnostics. PCR enables the synthesis of nucleic acids in vitro through which a DNA segment can be specifically replicated in a semiconservative way that sets forth deletion and mutation analysis. Multiplex PCR (M-PCR) is beneficial over standard and long PCR as this can amplify more than one locus using the respective primer sets. In harmony with this, the present study aimed to optimize M-PCR followed by its chemistry and condition to screen Duchenne Muscular Dystrophy (DMD) [OMIM #310200] and Becker Muscular Dystrophy (BMD) [OMIM #300376]. Muscular Dystrophies (MDs) are a broad group of hereditary, progressive, and degenerative disorders of muscles. X-linked recessive D/BMD are caused by mutation/s in the dystrophin gene [OMIM #300377] that encodes for dystrophin protein [UniProt#P11532]. As dystrophin is the human metagene with 79 exons, mutational analysis is very challenging. Chamberlain set (10 plex), Beggs set (9 Plex), and Kunkel set (7 Plex) is used for many years to diagnose this condition. However, in this study, Beggs set is customized with 13 exons to screen DMD gene mutation in a single reaction. Optimization of M-PCR was designed with many physicochemical parameters. According to the literature and after many appraisals the present study demonstrated the most sufficient concentration of various chemical components and optimal cycling conditions to optimize the modified Beggs set (13 Plex). 50 µL PCR reaction includes primer(s) (0.3–0.5 µM each), dNTP mixture (160 µM each), Dream Taq buffer (1X), Taq DNA polymerase (6U/50 µL), DNA template (250 ng/50 µL), BSA (0.4 µg/µL), and MgCl2 (1.4 mM). To get the most effective results cyclic conditions obtained were 10 min initial denaturation at 94°C, 62°C annealing temperature, and 35 PCR cycles at 72°C extending temperature. Consequently, the study successfully formulated a less expensive and simple approach for >3000 bp that was used to screen D/BMD. Finally, a developed M-PCR mix with a unique combination of specificity and sensitivity coupled with great flexibility has led to a true revolution in molecular diagnostics.

The influence of ultrasonic activation on the setting time and flow of four endodontic sealers / A influência da ativação ultrassônica no tempo de ajuste e no fluxo de quatro vedadores endodônticos / La influencia de la activación ultrasónica en el tiempo de ajuste y flujo de cuatro selladores endodónticos

The aim of the present study was to evaluate the influence of ultrasonic activation (UA) on the setting time and flow of four endodontic sealers: AH Plus (AH), Sealer Plus (SP), MTA Fillapex (MTAF), and BioRoot RCS (BIO). Properties were evaluated as required by ANSI/ADA Specification N° 57 (2008); only the size of the specimens was modified. UA was applied using a smooth tapered ultrasonic tip coupled to a piezoelectric ultrasonic device (30% power) on the freshly mixed materials in two cycles of 20 seconds. The results were statistically analyzed using the ANOVA and Kruskal-Wallis tests, followed by the Tukey and Dunn posthoc tests, respectively, depending on the normality of the data. The shortest setting times, initial and final, were, 115 (BIO/UA) and 148.6 (BIO/UA) min, whereas the longest were 1215 (AH) and 1928 (AH) min. The MTAF sealer did not set throughout the experimental period (2880 minutes). Significant differences were observed between BIO and MTAF and the other sealers, with or without UA, both in the initial and final setting time (P < 0.05). UA did not change the initial setting times; however, it reduced the final setting of BIO (P < 0.05). The highest and lowest flow values observed were 25.52 mm (AH/UA) and 18.66 mm (BIO/UA), respectively. The AH sealer, regardless of UA, exhibited higher flow values compared to the other sealers (P < 0.05), except for the MTAF/UA group, which was the only sealer in which UA promoted a significant flow increase (P < 0.05). Under the conditions of the study, it can be concluded that the BIO, under UA, presented the lowest setting time; however, it exhibited the lowest flow values. The MTAF sealer did not reach its final setting. Moreover, the SP groups exhibited intermediate results in all analyses. In summary, only the final setting time of the BIO group and the flow values of the MTAF group were influenced by UA.

O objetivo do presente estudo foi avaliar a influência da ativação ultrassônica (UA) no tempo e fluxo de ajuste de quatro selantes endodônticos: AH Plus (AH), Sealer Plus (SP), MTA Fillapex (MTAF) e BioRoot RCS (BIO). As propriedades foram avaliadas conforme exigido pela Especificação N° 57 (2008) da ANSI/ADA; apenas o tamanho dos espécimes foi modificado. O UA foi aplicado usando uma ponta de ultrassom suave cônica acoplada a um dispositivo ultrassônico piezoelétrico (30% de potência) nos materiais recém-misturados em dois ciclos de 20 segundos. Os resultados foram analisados estatisticamente usando os testes ANOVA e Kruskal-Wallis, seguidos pelos testes póstticos de Tukey e Dunn, respectivamente, dependendo da normalidade dos dados. Os tempos de ajuste mais curtos, iniciais e finais, foram 115 (BIO/UA) e 148,6 (BIO/UA) min, enquanto os mais longos foram 1215 (AH) e 1928 (AH) min. O selador da MTAF não foi colocado durante todo o período experimental (2880 minutos). Foram observadas diferenças significativas entre o BIO e o MTAF e os demais seladores, com ou sem UA, tanto no tempo de ajuste inicial quanto final (P < 0,05). A UA não alterou os tempos de ajuste inicial; no entanto, reduziu o ajuste final da BIO (P < 0,05). Os valores de fluxo mais alto e mais baixo observados foram 25,52 mm (AH/UA) e 18,66 mm (BIO/UA), respectivamente. O selador AH, independentemente do UA, apresentou valores de fluxo mais elevados em comparação com os outros seladores (P < 0,05), exceto para o grupo MTAF/UA, que foi o único selador no qual o UA promoveu um aumento significativo do fluxo (P < 0,05). Nas condições do estudo, pode-se concluir que o BIO, sob UA, apresentou o menor tempo de ajuste; no entanto, exibiu os menores valores de fluxo. O selador MTAF não atingiu seu ajuste final. Além disso, os grupos de SP apresentaram resultados intermediários em todas as análises. Em resumo, apenas o tempo de ajuste final do grupo BIO e os valores de fluxo do grupo MTAF foram influenciados pelo UA.

El objetivo del presente estudio fue evaluar la influencia de la activación ultrasónica (AU) sobre el tiempo de ajuste y flujo de cuatro selladores endodónticos: AH Plus (AH), Sealer Plus (SP), MTA Fillapex (MTAF) y BioRoot RCS (BIO). Las propiedades se evaluaron según lo requerido por la especificación ANSI/ADA N° 57 (2008), sólo se modificó el tamaño de los ejemplares. El AU se aplicó utilizando una punta ultrasónica cónica lisa acoplada a un dispositivo piezoeléctrico ultrasónico (30% de potencia) sobre los materiales recién mezclados en dos ciclos de 20 segundos. Los resultados se analizaron estadísticamente mediante las pruebas ANOVA y Kruskal- Wallis, seguidas de las pruebas postcográficas de Tukey y Dunn, respectivamente, dependiendo de la normalidad de los datos. Los tiempos de fraguado más cortos, inicial y final, fueron 115 (BIO/UA) y 148,6 (BIO/UA) min, mientras que los más largos fueron 1215 (AH) y 1928 (AH) min. El sellador MTAF no se ajustó durante todo el período experimental (2880 minutos). Se observaron diferencias significativas entre BIO y MTAF y los demás selladores, con o sin AU, tanto en el tiempo de ajuste inicial como final (P < 0,05). La AU no modificó los tiempos de ajuste inicial, pero redujo el ajuste final de BIO (P < 0,05). Los valores más altos y más bajos de caudal observados fueron 25,52 mm (AH/UA) y 18,66 mm (BIO/UA), respectivamente. El sellador AH, independientemente del AU, presentó valores de caudal más altos en comparación con los demás selladores (P < 0,05), excepto para el grupo MTAF/AU, que fue el único sellador en el que el AU promovió un incremento significativo del caudal (P < 0,05). Bajo las condiciones del estudio, se puede concluir que el BIO, bajo AU, presentó el menor tiempo de fraguado, sin embargo, presentó los menores valores de caudal. El sellador MTAF no alcanzó su ajuste final. Por otra parte, los grupos SP presentaron resultados intermedios en todos los análisis. En resumen, solo el tiempo de ajuste final del grupo BIO y los valores de flujo del grupo MTAF fueron influenciados por el AU.

Microbiological and Nutritional Quality of Complementary Foods for Children (6 to 24 months) Produced in the City of Man (Côte d'Ivoire)

This study aims to determine the microbiological and nutritional quality of complementary foods for children produced in the city of Man (instant flour and porridge). To do this, a structured survey of nannies and vendors, coupled with microbiological and physicochemical analyzes was carried out. To this end, a questionnaire survey was conducted among nannies and porridge sellers in order to collect information on the use of complementary foods and to assess the state of cleanliness of the sales environment. A study of physicochemical (carbohydrates, lipids, humidity, ash, minerals) and microbiological (total aerobic mesophilic flora, yeasts and molds, total coliforms, Escherichia coli, Staphylococcus sp. and Salmonella sp.) characteristics using standard methods and referenced was then conducted to assess the nutritional value and health status of the porridges (Millet, Kokobaka, Anagobaka) and flours from the Focolari center consumed in Man. The survey data showed that the majority of the of nannies (61.1%) give a complementary food before the age of introduction (6 months), with regard to the saleswomen (100%) unaware of the proportion of the components and their nutritional intake as well as 54.8% of them have an unhealthy work place and environment. The microbiological analysis carried out on the 50 samples revealed a potential contamination of spoilage and pathogenic germs. The contamination rate of Anagobaka porridge is very worrying unlike other porridges and flours, especially with the presence of salmonella, the high rate of Escherichia coli and Staphylococcus aureus respectively (3.91×103 ± 0.09 and 5.34×104 ± 0.10 CFU/mL).) Regarding the nutritional analysis, there is a content of carbohydrates (70.17-85.08%), lipids (6.93-10.40%) and iron (30.64-64.34%) higher than those recommended by the FAO/WHO standard in the 6 types of samples analyzed. However, the zinc content is below this standard in all the samples tested. However, zinc deficiency is one of the main causes of stunted growth in children. All these results have shed light on the existence of a potential health risk and possible nutritional deficiencies in infants and young children who consume locally produced complementary foods.

Physico Chemical Analysis and HPTLC Evaluation of Kokilakshadi Kwatha Churna

Kokilakshadi Kashaya mentioned in Bhaishajya Ratnavali, Vataraktadhikara, it is a therapeutic formulation to treat Vatarakta. It is also used by Ayurvedic practitioners for treating hyperuricemia. The symptoms of hyperuricemia and gouty arthritis are similar to Vatarakta, a disease explained in classical Ayurvedic textbooks. Kokilakshadikwatha contains Kokilaksha and Guduchi and Pippalichurna given as Anupana of this formulation Physico chemical analysis of individual drug and formulation with modern parameters increase their scope and acceptance. The study was based on standard analytical parameters proposed by API. Method: Kokilakshadi Kwatha powder was evaluated for physico chemical analysis and phyto chemical screening. The analysis was done by using the parameters like Organoleptic features, loss on drying, acid soluble extractive, water soluble extractive. Results: Analytical parameters of individual drugs were done. All analytical parameter were within limit. Analytical parameter of Kokilakshadi Kwatha Churna like loss on drying 10.4%w/w, acid insoluble ash 0.79%, alcohol soluble extractive 11.2%w/w, water soluble extractive 7.8%w/w, pH 5.78 were obtained. High Performance Thin Layer Chromatography (HPTLC) profile of Kokilakshadikwatha powder showed 13 peaks at 254nm and 14 peaks at 366nm. Preliminary phytochemical screening test revealed the presence of steroids, alkaloids, phenols, flavonoids. Conclusion: The obtained data can be used for future comparative references

Analytical Study of Natakusthadi Yoga with special reference to Their Vishaghna Effects

Nata & Kushta medicinal plants are easily available in Indian diaspora and both are widely used in many Agada as Vishaghna dravya mentioned in classical texts. Both have reported acetylcholinesterase and Phospholipase A2 enzyme inhibiting property in many Research studies. The quality of Nata, Kustha & Nata Kustha Yoga was evaluated based on organoleptic, physico-chemical, and microbial quality parameters. Loss on drying at 105°C, Total ash, Acid Insoluble ash, Alcohol-soluble extractive, Water-Soluble extractive, HPTLC and pH value parameters were evaluated as per WHO guidelines, Ayurvedic pharmacopoeia and Indian pharmacopoeia. Analytic analysis of Nata, Kustha & Nata Kusthadi Yoga in this study found to be under standard limit. Standardization test done on Natakusthadi Yoga helped in authenticating the preparation and in ensuring quality of the same. The Natakusthadi Yoga prepared by following standard operative procedures should have Loss on drying at 105°C, Total ash, Acid Insoluble ash, Alcohol-soluble extractive, Water-Soluble extractive and pH value is 2.1%, 4.8%, 0.32%, 42.9%, 37.8% & 5.5 respectively

FISH SPECIES DIVERSITY OF KOLLERU LAKE AT RAMSAR OF ELURU DISTRICT ANDHRA PRADESH, INDIA

During the study period we recorded 29 species The fish production plays a significant role in the human economy. India has vast potential for development of inland fisheries. In the present study an attempt has been made to evaluate the physico-chemical characteristic features and fish fauna diversity of Kolleru Lake Eluru District. It proposed to enhancement of fish production in a sub urban perennial water body in Eluru district of Andhra Pradesh. The study was conducted during May 2021 to April 2022. The study revealed that different regions of the tank receive variable precipitation and hence meteorological factors governing the physico-chemical properties of the tank which in turn influence planktonic diversity and so that all the physico-chemical and biological factors have direct influence on the fish production. The present study, it observed that all the physico-chemical parameters are at nearly permissible limit at all the 3 identified stations. It is estimated that the fish fauna of Kolleru Lake consists of 29 species belonging to 12 families. Among the collections 10 species of Cypriniformes, order Siluriformes consists of 5 species, Channiformes consists of 05 species, Perciformes 03 species, and Osteoglossiformes 01 species. Therefore, it is suggested that the immediate measures may be to avoid further contamination of this Lake which has been started contaminated due to anthropological activities. Finally it may be concluded that the Kolleru Lake is rich in fish diversity and is still in a position to set a good example of conservation of native fish fauna by the sustainable management practices.

Pharmaceutical Standardization and Physicochemical Analysis of Arogyavardhini Vati

It is very crucial to know the physicochemical properties of drugs during the development of pharmaceutical products. Drugs are on compulsion to assess their compatibility of active substances, excipients and medicinal products with established standards by the pharmaceutical laws. Characterization of these active pharmaceutical ingredients (APIs) helps in improving the quality parameters of all raw materials used during the manufacturing process of pharmaceuticals and also in the final products. Maintaining quality standards of the drugs is the need of the hour in this era of increasing demand for indigenous medicines. Due to the absence of reference standards, standardisation of many of compound formulations is lagging behind. Ayurveda is one of the oldest medical science that has been serving the community since centuries. Arogyavardhini vati is one of the most important Ayurvedic formulation that is advised by the Ayurvedic scholars for liver disorders since centuries. Although, being administered by a vast community of Ayurvedic practitioners and from a very long period with multiple benefits, there were no many studies that are available on the physicochemical analysis and standardization of Arogyavardhini vati. Present study evaluated the physicochemical properties of Arogyavardhini vati and standardized. Arogyavardhini vati prepared by the Ayurvedic classical method complies with the standard parameters as mentioned in Ayurvedic Pharmacopeia of India. Present study observed that the analytical parameters and the pharmaceutical parameters for Arogyavardhini Vati were validated by HPTLC method and can be considered as the standard drug.

SEASONAL VARIATION IN WATER QUALITY AND PHYTOPLANKTON PRODUCTION IN JOHILA RESERVOIR, LALPUR, AMARKANTAK (M.P.)

Present study has been undertaken in order to assemble knowledge regarding physicochemical parameters analysis from June 2021 – May 2022 in relation to the abundance, composition and seasonal variation in the phytoplankton of Johila reservoir Lalpur Podki, Amarkantak (M.P.). It is interiorly located at Latitude 22046'20, Longitude, 81044'39'' in Lalpur and no study regarding hydrobiology in general and phytoplankton abundance in particular has yet not been conducted. Johila reservoir has its origin from Jwaleshwar temple kund of Amarkantak, hill located in Makel velly. It starts flowing opposite site to the Narmada River after 15 Kilometre and finally joins the river Sone at Dasrathghat of Umaria District of Madhya Pradesh. Total area of Johila reservoir is 86 hectare (216 acers). Since no study yet has been done on phytoplankton production in relation to its physico-chemical characteristic hence present study has been undertaken. Phytoplankton population were dominated by Chlorophyceae, Myxophyceae and Bacillariophyceae in decreasing order. Chlorophyceae members revealed dominance over Myxophyceae and Bacillariophyceae.

Physico-Chemical Analysis of Panchagavya Ghritha Prepared as per Ashtanga Hridaya Reference and Comparison with Market Samples

Panchagavya Ghritha is a widely used Ayurvedic formulation mentioned in Ashtanga Hridaya Utharastana Apasmara Patishedha indicated in conditions like Apasmara (epilepsy), Jwara (pyrexia), and Kamala (jaundice). It contains 5 ingredients namely Goshakrit rasa (cow dung juice), Amladadhi (sour curd), Goksheera (cow’s milk), Gomutra (cow’s urine) and Goghritha (ghee). Authentic sources of cow products are not often obtained and found adulterated. Collection and processing of fresh raw materials are an important area in this formulation This study was conducted to find out the non conformances and quality issues in Panchagavya ghritha production in industrial level. The comparative analysis of prepared and market samples based on standard analytical parameters proposed by PLIM reveals the variation in different organoleptic characters and physico-chemical parameters. The physico-chemical parameters among prepared and different companies were statistically analysed with ANOVA test and Scheffe’s pair wise comparison, showed significant difference at 0.01 levels.

Physico Chemical Analysis of Asanavilwadi Tila Tailam and Asanavilwadi Kera Tailam

Taila Kalpana- A liquid dosage form of Ayurveda used for both external and internal application, has an important role in clinical practice. Asanavilwadi taila is a preparation mentioned in Sahasrayogam taila prakaranam and in Chikitsa manjari Siro roga chikitsa. It is used in the treatment of diseases of eye, ear and head and found to be very effective. Based on the data collected about the production of Asanavilwadi tailam from various manufacturing companies, it was noted that large amount of Asanavilwadi tila tailam and Asanavilwadi kera tailam are produced. In the present scenario there is increase in the number of manufacturing companies, increased production of formulations and there is decreased availability of raw drugs. So, it is necessary to confirm the genuinity of formulations available in the market. But Asanavilwadi tailam has not been standardized yet in API. This work was initiated to develop a standard analytical parameter for Asanavilwadi tila tailam and Asanavilwadi kera tailam. Asanavilwadi taila is prepared both in the media of Tila taila and Kera tailam as both samples are used for clinical practice and physico-chemical analyses were done. Standard analytical protocol proposed by Pharmacopoeial Laboratory of Indian Medicine (PLIM) and Ayurvedic Pharmacopoeia of India were taken as the study tool.

Physico-chemical and Microbiological Qualities of Abattoir Wastewater in Egbu, Imo State, Nigeria

Abbattoir waste water is of a complex composition. When discharged without being treated, poses a threat to human health and the environment. This is the practice common in Nigeria, and is worrisome.Objective: The aim of this study was to assess the physico-chemical and microbiological qualities of waste water from Egbu abattoir discharged into ‘Otamiri’ river in Owerri North local government area, Imo State, Nigeria.Methods: The microbial quality of abattoir wastewater was studied. The duration of the study was three months, from June to September, 2019. Sample collection was done in the morning (8-10am) and in the evening (4-6pm). A total of thirty samples (30) were collected from two sampling points, at the place of generation of the wastewater (Effluent source) and at the point of discharge into the river (POE). Standard methods were employed to assess the microbiological and physiochemical quality of the effluent. The microorganisms isolated from the samples included Escherichia coli, Salmonella, Staphylococcus, Aspergillus, Mucor, Listeria, Micrococcus and Candida species. The Total Heterotrophic Count (THC) of effluent samples, at source and POE were 8.51log10 cfu/ml and 6.15log10cfu/ml respectively, Total Coliform Count (TCC) for same samples were 6.77log10 cfu/ml and 4.56log10 cfu/ml respectively. The Total Fungal Count (TFC) of the effluent samples at source and at POE was 5.19log10 cfu/ml and 4.18log10 cfu/ml respectively. Results further revealed that the pH of the effluent at source was 6.58 while that at Point of Entry into the river was 7.30. The temperature of both samples was 25oC. The Total Dissolved Solid values of the effluent collected at source was higher, having a value of 1400mg/L against 1000mg/L at Point of Entry. Also the Biochemical Oxygen Demand (BOD) and Chemical Oxygen Demand (COD) values of the effluent at source were 5.10mg/L and 8.58mg/L respectively, while the BOD and COD of the water at the POE were 4.3mg/L and 7.9mg/L respectively.Conclusion: Abattoir wastes are becoming a major environmental health challenge and being discharged into the Otamiri River indiscriminately is of public health concern. The potential public health implications associated with discharging untreated abattoir wastewater into the environment and thus, the need for adequate treatment to ensure decontamination as well as providing wastewater treatment facility is imperative.

Correlation of Limnological Features with Ichthyofauna in Hatnur Reservoir in Jalgaon, Maharashtra, India

Background: Limnologycovers the study of all inland waters including running and standing waters, fresh and saline, natural or man-made reservoirs. Unplanned urbanization, rapid industrialization and indiscriminate use of artificial chemicals lead to heavy and varied pollution in aquatic environments causing to deterioration of Limnological features and depletion of aquatic fauna including fish. Scarcity of research related to limnological features and ichthyofauna diversity in Hatnur reservoir stimulated to undertake the present work. Methods: River Tapi (or Tapti) is arising in Madhya Pradesh and flows westward between two spurs of the Satpuda Rangeacross Maharashtra state, and through Gujarat state to the Gulf of Khambhat. Hatnur reservoir (750 90扙, 210 12扤) formed due to an earth-fill dam on Tapi river near Hatnur village in Jalgaon district of Maharashtra state. Water quality parameters were assessed using standard methods described by APHA and fish diversity was evaluated in the Hatnur reservoir. Results: Seasonal fluctuations in water quality parameters and their relationship with ichthyofauna inhabiting the Hatnur reservoir were assessed. The results obtained in the present study indicated that physicochemical properties of water samples collected from the Hatnur reservoir were within recommended limits of the Bureau of Indian Standards (BIS). Conclusion: It is concluded that water in Dam was less polluted, suitable for agricultural and domestic use and suitable to inhabit the fish diversity. Future studies should focus on under-explored and unexplored areas of the Tapi riverine system in North Maharashtra to comprehensively document the fish diversity.

Simulation of density, viscosity and ultrasonic velocity data of 3-bromoanisole + methanol mixtures at different temperatures

SUMMARY Aim: To represent mathematically the reported physico-chemical properties (PCPs) of binary mixtures of 3-bromoanisol + methanol at various temperatures by using a single model with seven curve-fit parameters. Results: Besides the correlation models, the applicability of training the proposed model using a minimum number of experimental data and prediction of the rest of data points with acceptable prediction error is also shown.

Objetivo: representar matemáticamente las propiedades fisicoquímicas (PCP) de mezclas binarias de 3-bromoanisol + metanol a varias temperaturas, utilizando un modelo único con siete parámetros de ajuste de curva. Resultados: además de los modelos de correlación, también se muestra la aplicabilidad del entrenamiento del modelo propuesto utilizando un número mínimo de datos experimentales y la predicción del resto de puntos de datos con un error de predicción aceptable.

Objetivo: representar matematicamente as propriedades físico-químicas reportadas (PCPs) de misturas binárias de 3-bromoanisol + metanol em várias temperaturas usando um único modelo com sete parâmetros de ajuste de curva. Resultados: além dos modelos de correlação, a aplicabilidade do treinamento do modelo proposto usando um número mínimo de dados experimentais e previsão do restante dos pontos de dados com erro de previsão aceitável também é mostrada.

Technological potential of under-utilized starches from eight varieties of legumes grown in Cameroon / Potencial tecnológico de amidos subutilizados de oito variedades de leguminosas cultivadas nos Camarões

Abstract Starch samples from eight legumes cultivars instar of one variety of Vigna unguiculata L. (Cowpea), one variety of Vigna subterrenea V. (Bambara groundnut) and six varieties of Phaseolus vulgaris L. (Common bean), grown in Cameroon were isolated, and their physicochemical and pasting properties were evaluated. The objectives of the study were to investigate the starch properties and processing characteristics of different bean varieties, and to establish the basic foundation of improving the functionality of beans and their starch grown in the region. The result revealed significant differences amongst the properties of the starches. The swelling power of the legume starch isolates put them in the category of highly restricted-swelling starch. This characteristic is desirable for the manufacture of value-added products such as noodles and composite blends with cereals. The pasting properties were determined using a rapid visco analyzer, and various legumes bean starches exhibited different pasting profiles. The high breakdown viscosity (BV) was founded for Cowpea and Bambara groundnut and confirmed their low. ability to resist heat and shear stress when compared to Common bean varieties studies. The factors which influence the pasting characteristics resulting to decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of starch are attributed to the interaction of starch with the protein, fat, etc. which depended to their variety.

Resumo Foram isoladas amostras de amido de oito cultivares de leguminosas ínstar de uma variedade de Vigna unguiculata L. (feijão caupi), uma variedade de Vigna subterrenea V. (amendoim Bambara) e seis variedades de Phaseolus vulgaris L. (feijão comum), cultivadas nos Camarões, e suas propriedades físico-químicas e de pasta foram avaliadas. Os objetivos do estudo foram investigar as propriedades do amido e as características de processamento de diferentes variedades de feijão e estabelecer as bases básicas para melhorar a funcionalidade do feijão e do amido cultivado na região. O resultado revelou diferenças significativas entre as propriedades dos amidos. O poder de inchamento dos isolados de amido de leguminosas os coloca na categoria de amido com inchamento altamente restrito. Esta característica é desejável para o fabrico de produtos de valor acrescentado, tais como macarrão e misturas compósitas com cereais. As propriedades de pasta foram determinadas usando um analisador rápido de visco, e vários amidos de feijão leguminosos exibiram diferentes perfis de pasta. A alta BV foi fundada para o amendoim Cowpea e Bambara e confirmou sua baixa. capacidade de resistir ao calor e tensão de cisalhamento quando comparado com estudos de variedades de feijão. Os fatores que influenciam as características colantes resultantes da diminuição do pico de viscosidade (PV), da viscosidade mínima (TV) e da viscosidade final (FV) do amido são atribuídos à interação do amido com a proteína, gordura, etc., que dependem da sua variedade.

Nitrogen fertilization associated with cold storage and its impacts on the maintenance of peach quality / Adubação nitrogenada associada ao armazenamento refrigerado e seus impactos na manutenção da qualidade do pêssego

Nitrogen, which is considered the most important nutrient for peach trees, may interfere in both quantitative production characteristics and quality of fruits. This study aimed at evaluating the effect of the combination of doses of N fertilization and different periods of cold storage on physico-chemical and phytochemical characteristics of peaches in post-harvest. The experiment had a randomized complete block design in a 4x3 factorial scheme, i. e., four doses of fertilization (0, 60, 120 and 180 Kg N ha-1) and three periods of storage (on the harvest day, on both the 15th and the 30th storage days at 1±1ºC, each followed by a day of simulated commercialization at 20±1ºC). The following aspects were evaluated in fruits yielded by peach trees of the genotype Cascata 1067: fruit color, soluble solid content, titratable acidity, pulp firmness, mass loss, total phenolic compounds and antioxidant activity. Different periods of cold storage and doses of N fertilization were found to affect epidermis luminosity, pulp firmness and titratable acidity of fruits. Peaches may be stored at low temperatures for 15+1 days. After that, loss of fruit firmness increases. N fertilization affects neither the soluble solid content nor the epidermis color of peaches, but both parameters are influenced by storage. Values of phenolic compounds and antioxidant activity decrease when there is N increment in the soil and when longer storage is carried out. Results suggest that peach composition may be affected by cultural practices, such as N fertilization, in harvest and after storage.

O nitrogênio é o nutriente considerado de maior importância para o pessegueiro, podendo interferir nas características quantitativas da produção, bem como na qualidade dos frutos. O objetivo do trabalho foi avaliar o efeito da combinação de doses de adubação nitrogenada e de diferentes períodos de armazenamento refrigerado nas características físico-químicas e fitoquímicas de pêssegos na pós-colheita. O delineamento experimental foi o de blocos casualizados em esquema fatorial 4x2, quatro doses de adubação (0, 60, 120 e 180 Kg N ha-1) e três períodos de armazenamento (dia da colheita, aos 15 e 30 dias armazenamento refrigerado a 1±1ºC, seguido de um dia de simulação de comercialização a 20±1ºC). Avaliou-se, nos frutos de pessegueiro do genótipo Cascata 1067, a coloração dos frutos, o teor de sólidos solúveis, acidez titulável, firmeza da polpa, perda de massa, compostos fenólicos totais e a atividade antioxidante. Verificou-se que diferentes períodos de armazenamento refrigerado e doses de adubação nitrogenada afetam a luminosidade da epiderme, a firmeza de polpa e a acidez titulável dos frutos. Os pêssegos podem ser armazenados em baixa temperatura até 15+1 dias, após esse período, a perda de firmeza nos frutos é elevada. A adubação nitrogenada não altera o teor de sólidos solúveis e a coloração da epiderme dos pêssegos, mas esses parâmetros são influenciados durante o armazenamento. Os compostos fenólicos e atividade antioxidante decrescem com o incremento de nitrogênio no solo, bem como durante o avanço dos dias de armazenamento. Os resultados sugerem que a composição dos pêssegos pode ser afetada com práticas culturais como a adubação de nitrogênio na colheita e após o armazenamento.

Quality Characteristics of Chicken Meat Cutlets Incorporated with Finger Millet (Eleusine coracana) Flour

In view of continuously growing demand of snacks foods worldwide due to rapid changing lifestyle, industrialization and urbanization, the present study was conducted to standardize the incorporation level of finger millet flour for preparation of chicken meat cutlets. Chicken meat cutlets were prepared with different levels of finger millet flour viz. 5, 10, and 15%, and different levels by replacing the lean meat in chicken cutlets formulation. The optimum (mention) level of finger millet was standardized as 5% for incorporation in chicken meat cutlets. Crude fiber and calcium content of chicken cutlets prepared with finger millet flour was significantly higher as compared to control thereby depicting marked improvement in the desirable food trait with health benefits. The product yield of chicken meat cutlets with optimized level of finger millet flour was significantly higher as compared to control. It did not show significant effect on any of the sensory parameters. Storage stability of aerobically packaged chicken meat cutlets with optimum level (mention level) of finger millet flour along with control was further evaluated during refrigerated storage (4±1oC) at regular intervals of 0, 3, 6 and 9 days with respect to physico-chemical, microbiological and sensory characteristics. Chicken meat cutlets could be stored safely in aerobic packaging for 6 days at refrigeration condition (4±1oC).