RESUMO
Objective To analyze relevant mechanism of clinical biochemical test results with blood specimen placement time,and provide reference for clinical examination.Methods100 biochemical test specimen in our hospital were selected, Biochemical test data were analyzed at different storage time (1h, 3h, 6h, 24h) using automatic biochemical analyzer combined with electrolyte analyzer.ResultsThe results of blood 1H determination were taken as the test standard, and the blood samples were placed in 3h.The results showed that the contents of glucose and carbon dioxide were significantly decreased (P<0.05).Blood samples were placed 8h, serum potassium, glucose, carbon dioxide, alanine aminotransferase (P<0.05) were significant differences, blood specimens were placed 24h, there were significant differences of the indexes including blood glucose, potassium, carbon dioxide, alanine aminotransferase, alkaline phosphatase, total bilirubin, total protein, albumin, aspartate aminotransferase, serum potassium, alpha hydroxybutyrate dehydrogenase, lactate dehydrogenase (P<0.05).ConclusionThe blood sample placement time have a significant impact on some biochemical indicators, in view of the unstable factors should be detected in the blood sample 1h intact, to avoid delay condition assessment.
RESUMO
Objective To study the influence of different placing time of child peripheral blood on blood routine parameters in order to increase the working efficiency of clinical blood routine test.Methods Each 300 μL of peripheral blood was collected from the right hand ring finger in 50 healthy children in our hospital from January 2015 to January 2016.The blood routine was performed by using the whole blood cells analyzer at instantly after blood collection,at 0,5,10,15,30 min after blood collection under the room temperature (20 to 25 ℃).Results With the measured results at 15 min as the control,the detection results at the other times points had no statistical difference (P>0.05).Compared with the detection results at 5 min,the white blood cell count(WBC) and platelet volume distribution width (PDW),lymphocyte absolute value (LYM),neutrophil percentage absolute value (NEU),platelet count (PLT),red blood cell volume distribution width (RDW),hemotocrit (HCT) and plateletcrit(PCT) were statistically different (P<0.05).Conclusion Reasonably arranging time,eliminating pre-analysis error and reducing the influence of peripheral blood placing time on blood routine parameters have an important significance to accurately judge the clinical dat.It is recommended that the blood routine detection time should be controlled within 10-30 min in order to increase the working efficiency of clinical detections.
RESUMO
Objective To explore the division XL automatic urine analyzer reagent strips placed time impact on nitrite (NIT) test results,to ensure that provide accurate and reliable experimental data for clinical,and improve clinical diagnostic rate. Methods Used a new load reagent storehouse article in the new reagents respectively in <5,30 min;1,2,3,3.5,4,4.5,5, 5.5,6,6.5,7 and 8 h,in different period,16 0.9 g/dl saline NIT in the false positive rate,and carried on the comparison to different times NIT false-positive rate analysis.Results <3.5 h NIT false positive rate was 0% (0/16),4~4.5 h false pos-itive rate was 18.8% (3/16),5~5.5 h false positive rate was 43.8% (7/16),6~6.5 h was 62.5% (10/16),7~8 h was 87.5% (14/16).More than 4 h each time comparison between NIT false positive rate,differences were statistically signifi-cant (χ2 =11.7~59.2,all P <0.01).Conclusion Division XL automatic urine analyzer reagent strips placed after 4 h,NIT false-positive rate started to rise,NIT false-positive rate was significantly increased after 5 h.Therefore,strengthen the divi-sion XL automatic urine analyzer reagent strips placed reasonable time management,should be in the < 3.5 h advisable,it helps reduce the NIT false positive rate.