Association of high blood pressure with raised homocysteine level among urban population- A case control study

Introduction: Hypertension is a major risk factor for cardiovascular diseases. Raised homocysteine level is also an important independent risk factor for CVD. This study sought to determine whether there is any rela?onship between plasma homocysteine and blood pressure levels. Method: A case control study was conducted among 200 hypertensive cases and 200 normal healthy control groups. Cases were from the Department of medicine and hypertensive clinics at GMC, Barama? and similar controls were selected from pa?ents’ neighborhood. Detailed clinical assessment as well as plasma homocysteine level were assessed and compared in both. Results: Hypertensive cases had higher mean homocysteine level (21.3 ± 4.6 µmol/L) from controls (13.0 ± 6.0 µmol/L), p<0.001. Homocysteine is posi?vely correlated with both systolic and diastolic blood pressure among both hypertensive pa?ents and healthy controls. In both hypertensive subjects and healthy control, homocysteine level has weak posi?ve correla?on with DBP and moderate to strong posi?ve correla?on with SBP. The hypertensive cases had very high chance (OR=52.4) of developing hyperhomocysteinemia (>15 µmol/L), p<0.001. Conclusion: This study showed higher mean plasma homocysteine levels in hypertensive subjects than controls. Serum homocysteine concentra?ons were posi?vely associated with both systolic and diastolic blood pressure levels in a general adult popula?on.

Effect of / 生物医学与环境科学（英文）

Objective@#This study aimed to investigate the effects of @*Methods@#In this study, 0.1% DMG was supplemented in 20% casein diets that were either folate-sufficient (20C) or folate-deficient (20CFD). Blood and liver of rats were subjected to assays of Hcy and its metabolites. Hcy and its related metabolite concentrations were determined using a liquid chromatographic system.@*Results@#Folate deprivation significantly increased pHcy concentration in rats fed 20C diet (from 14.19 ± 0.39 μmol/L to 28.49 ± 0.50 μmol/L; @*Conclusion@#DMG supplementation exhibited hypohomocysteinemic effects under folate-sufficient conditions. By contrast, the combination of folate deficiency and DMG supplementation has deleterious effect on pHcy concentration.

Effect of mecobalamin on diabetic peripheral neuropathy and its effect on plasma homocysteine / 中国生化药物杂志

Objective To study the correlation analysis of mecobalamin in treatment of diabetic peripheral neuropathy and its plasma homocysteine level. Methods 100 patients were selected from January 2013 to August 2016 treatment of diabetic peripheral neuropathy, according to admission ID, were randomly divided into control group and observation group, each group of 50 people. At the same time, 50 healthy persons were collected as normal control group and two groups were given routine treatment of Western medicine. The observation group was given mecobalamin. The plasma levels of plasma homocysteine were detected in the three groups, and the improvement degree of neuropathy was assessed by clinical and electrophysiological methods. Results The observation group and the control group of patients before treatment, plasma homocysteine levels were significantly higher than normal control group; the observation group with Mecobalamin after treatment of plasma homocysteine level in patients were significantly better than the control group (P<0.05); through clinical and electrophysiological evaluation method of observation group of patients in the neuropathic symptoms and nerve conduction velocity is the control group improved significantly (P<0.05). Conclusion The levels of plasma homocysteine and diabetic peripheral neuropathy have a strong correlation, and the use of mecobalamin in treating diabetic peripheral neuropathy can decrease homocysteine levels in patients with, and is effective for the treatment of diabetic peripheral neuropathy, and can be widely applied in clinical treatment.

Effect of mecobalamin on diabetic peripheral neuropathy and its effect on plasma homocysteine / 中国生化药物杂志

Objective To study the correlation analysis of mecobalamin in treatment of diabetic peripheral neuropathy and its plasma homocysteine level. Methods 100 patients were selected from January 2013 to August 2016 treatment of diabetic peripheral neuropathy, according to admission ID, were randomly divided into control group and observation group, each group of 50 people. At the same time, 50 healthy persons were collected as normal control group and two groups were given routine treatment of Western medicine. The observation group was given mecobalamin. The plasma levels of plasma homocysteine were detected in the three groups, and the improvement degree of neuropathy was assessed by clinical and electrophysiological methods. Results The observation group and the control group of patients before treatment, plasma homocysteine levels were significantly higher than normal control group; the observation group with Mecobalamin after treatment of plasma homocysteine level in patients were significantly better than the control group (P<0.05); through clinical and electrophysiological evaluation method of observation group of patients in the neuropathic symptoms and nerve conduction velocity is the control group improved significantly (P<0.05). Conclusion The levels of plasma homocysteine and diabetic peripheral neuropathy have a strong correlation, and the use of mecobalamin in treating diabetic peripheral neuropathy can decrease homocysteine levels in patients with, and is effective for the treatment of diabetic peripheral neuropathy, and can be widely applied in clinical treatment.

The relationship between plasma homocysteine and acute cerebral infarction and the effect of folic acid and VitB12 / 中国生化药物杂志

Objective To study the relationship between plasma homocysteine and acute cerebral infarction and the effect of folic acid and VITB12.Methods74 cases of cerebral infarction patients in Jiande Hospital of Integrated Chinese and Western Medicine from December 2015 to December 2016 were selected as the observation group, 42healthy patients who received physical examination at the same stage were selected as the control group.All the researchers are using fluorescence polarization immunoassay to measure the plasma levels of homocysteine.47 patients with hyperhomocysteinemia in patients with acute cerebral infarction, then according to the classification method of randomly divided into the treatment group(n=23) and non treatment group(n=24).The treatment group was treated with folic acid and vitamin B12, while those in the non treatment group were not treated with these drugs, the effect of the intervention was compared.ResultsThe level of plasma homocysteine in the observation group was significantly higher than that in the control group, the difference was statistically significant (P<0.05).The levels of plasma HCY in the treatment group were significantly decreased after treatment with folic acid and vitamin B12,compared with non treatment group, the difference was statistically significant (P<0.05).ConclusionIn patients with acute cerebral infarction and the plasma homocysteine level was significantly higher than normal, so it needs to be controlled in a reasonable range, folic acid and vitamin B12 can reduce plasma HCY of the patient's body, and keep it in a more reasonable range, for the prevention of acute cerebral infarction is significant, it is worthy of clinical application.

Relationship between Homocysteine Levels in Patients with Acute Ischemic Stroke and Discharged Outcome / 现代检验医学杂志

Objective To investigate the relationship between plasma homocysteine on admission and the outcome at discharge of acute ischemic stroke.Methods A non-concurrent cohort study was performed and a total of 1 3 1 9 patients with acute is-chemic stroke were continuously included in this study.According to tertile range of plasma homocysteine,patients were di-vided into three group.Logistic regression analysis was used to assess the independent association between plasma homocys-teine on admission and poor outcome at discharge of acute ischemic stroke.Results The difference of plasma homocysteine on admission between the poor outcome and those with good outcome had statistical significance (P<0.000 1).Without the adj ustment of multiple factors,when comparing to the first group,the second and third tertile seemed to have a tendency of increasing the risk of poor outcome at discharge,the OR (95%CI)was 2.111 (1.297~3.437,P<0.05),2.113 (1.361~3.279,P<0.05).After adjustment for multivariate,the second and third tertile also seemed to have a tendency of increasing the risk of poor outcome at discharge,the OR (95%CI)was 1.876 (1.160~3.036,P<0.05),2.396 (1.414~4.062,P<0.05).Conclusion The current study indicated that higher plasma homocysteine level was an independent risk factor for poor outcome at discharge in ischemic stroke patients.It would increase the risk of the outcome at discharge in patients with acute ischemic stroke,and suggests that there is a dose-response relationship between plasma homocysteine level on admis-sion and the poor outcome at discharge.

Treatment effect and impact on Hcy concentration of benserazide and senflor treating elderly patients with middle-late PD / 中国现代医生

Objective To investigate the treatment effect and impact on plasma homocysteine (Hcy) concentration of benserazide and senflor treating elderly patients with middle-late Parkinson's disease (PD). Methods 103 elderly pa-tients with middle-late PD were retrospectively analyzed in our hospital from March 2010 to May 2013,which divided into pure group and combined group according to treating method. Pure group was given dobutamine silk hydrazine treatment,combined group was given benserazide and senflor (pramipexole hydrochloride) treatment. Clinical efficacy was evaluated by UPDRS score and quality of life changes were rated by PDQ-39. Hcy before and after 6 months treatment of two groups was detected. Results After treated with 2,6 months, effective rate of the combined group to-tal was as high as 94.44%, was higher than the 69.39% of pure group, and the difference was statistically significant(P<0.05). PDQ-39 grading of the two groups after treatment were significantly lowered, and the PDQ-39 score of combined group was significantly lower than that of the pure group(P<0 . 05). The level of Hcy after treated with Benserazide for six months increased significantly, while Hcy levels had no further increase after treated with senflor. The level of Hcy in combined group after 6 months treatment was higher,without significant difference combined with the pure group (P>0.05). Conclusion Benserazide combined senflor treating middle-late PD can significantly improve the effect of treatment, improve the patient's quality of life, and do not increase high Hcy levels which is caused by benserazide,it's worth of further clinical promotion.

Elevated plasma homocysteine and carotid atherosclerosis in patients with cerebral infarction of the relationship analysis / 中国现代医生

Objective To elevated plasma homocysteine (Hcy) the relationship between carotid atherosclerosis and cere-bral infarction patients were studied. Methods 60 patients with acute cerebral infarction from September 2012 to September 2014 in our hospital were collected as the study group. The empty stomach blood, determination of Hcy, and the examination of the carotid artery colour was hospitalized for the next day to exceed, observed whether carotid atherosclerosis. At the same time, 60 cases of healthy subjects of the same period were examined as the control group. Results The two groups had statistical significance in hypertension, diabetes, low-density lipoprotein and Hcy (P<0.05), There was no difference in drinking history, smoking history, total cholesterol and triglyceride index (P>0.05), and mul-tivariate Logistic regression analysis showed that plasma Hcy levels and carotid atherosclerosis were positively correlated. Conclusion Elevated plasma homocysteine is a risk factor for atherosclerosis, and we have close ties and cerebral in-farction.

Homocisteína en mujeres menopáusicas de Lima / Homocystein levels in menopausal women from Lima, Peru

Objetivos: Comparar los niveles de homocisteína según el estado menopáusico en un grupo de mujeres atendidas en un hospital de Lima. Diseño: Estudio descriptivo y correlacional. Lugar: Centro de Investigación de Bioquímica y Nutrición, Facultad Medicina, Universidad Nacional Mayor San Marcos, Lima, Perú. Participantes: Mujeres de 40 a 60 años de edad. Intervenciones: En 97 mujeres de 40 a 60 años de edad atendidas en el Hospital San Bartolomé, Lima, Perú, se determinó el estado menopáusico en base a evaluación clínica y dosaje de FSH y estradiol en sangre. Para determinar la homocisteína plasmática se usó el método de inmunoanálisis de polarización de fluorescencia. Principales medidas de resultados: Niveles de homocisteína en sangre. Resultados: Los niveles de homocisteína hallados estuvieron dentro de los valores referenciales internacionales (5 a 15 µmol/L). Las mujeres posmenopáusicas presentaron niveles de homocisteína significativamente mayores comparados con las mujeres premenopáusicas (p<0,05). Conclusiones: Los niveles plasmáticos de homocisteína fueron mayores en las mujeres menopáusicas y su incremento podría ser considerado en la predicción de la enfermedad cardiovascular.

Objectives: To compare serum homocysteine levels according to menopausal status in a group of women. Design: Descriptive and correlational study. Setting: Biochemistry and Nutrition Research Center, Faculty of Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru. Participants: Women 40 to 60 year-old. Interventions: The menopausal status was determined clinically and by serum FSH and estradiol in 97 women 40 to 60 year-old attended at San Bartolome Hospital, Lima, Peru. Plasma homocysteine was determined by fluorescence polarization immunoanalysis. Main outcome measures: Homocysteine levels. Results: Homocysteine levels were within international referential values (5-15 µmol/L). Postmenopausal women levels were significantly higher compared to premenopausal women levels (p< 0.05). Conclusions: Homocysteine levels are increased in menopausal women and could be considered in cardiovascular disease prediction.

Relationship between the level of plasma homocysteine and coronary calcification in patients with different blood glucose levels / 中国综合临床

Objective To determine the relationship between the level of plasma homocysteine and coronary calcification in patients with different blood glucose levels. Methods By measuring plasma homocysteine and coronary calcification in 30 cases of diagnosed diabetes (T2D) ,29 cases of diagnosed impaired glucose tolerance (IGT) in patients and 27 cases with normal, we compared the level of plasma homocysteine and coronary calcification in patients with different blood glucose levels. Results We found significant diffieronces among three groups of the level of plasms homocysteine and coronary calcification (P ＜ 0.01). The plasma homocysteine levels were(19.31 ±3.17) μmol/L, (13.85 ± 1.62) μmol/L, (9.80 ± 1.78) μmol/L in the T2D,IGT and normal groups,respectively. The coronary calcification scores were 207.80 ± 154.10,63.24 ± 10.46,14.47 ± 5.16 in the T2D, IGT and normal groups, respectively. The plasma homocysteine level and coronary calcification score increased with the glycosylated hemoglobin rise in the normal,IGT and T2D groups((4.51 ±0.48)%, (6.13 ±0.31)% and (7.69 ±0.81)%, respectively). Conclusions The plasma homocysteine level is a strong independent predictor of type 2 diabetes and also an important factor of coronary artery event occurrence and develepment.

Relationships of Plasma Homocysteine Concentration and Oxidative Stress Markers in Korean Collage Students

Elevated plasma concentration of total homocysteine (ptHcy) is known as an independent risk factor of cardiovascular disease (CVD) and oxidative stress is also commonly implicated in CVD. An association between ptHcy and oxidative stress has recently been suggested. The study objective is to examine the relationship between ptHcy and oxidative stress markers in 103 healthy college students (62 males and 41 females). Plasma levels of ptHcy, oxidative stress markers (conjugated diene, erythrocyte catalase, TRAP, lymphocyte DNA damage), antioxidant vitamins (alpha-tocopherol, gamma-tocopherol, carotenoids), and lipid parameters (total cholesterol, triglyceride, HDL cholesterol) were determined. The results show that the concentration of ptHcy was significantly higher in male subjects (22.17 +/- 2.14 micromole/L) than in female subjects (12.28 +/- 0.45 micromole/L). There was a negative association between ptHcy and plasma beta-carotene in male subjects (p or = 15 micromol/L), as compared to those with lower plasma homocysteine. These study results confirmed the views on the association between plasma homocysteine and oxidative stress markers in humans and support the hypothesis that homocysteine promotes the oxidative environment by counteracting the antioxidant defense mechanism.

Clinical Significance of Plasma Homocysteine Level in Congestive Heart Failure / 上海第二医科大学学报

Objective To examine the relationship between plasma homocysteine level and status of congestive heart failure. Methods Plasma homocysteine level was determined in 106 patients with congestive heart failure(CHF).Among them,40 patients were diagnosed as having recent onset of CHF(group 1) and the remaining 66 were receiving conventional treatment(group 2).Thirty healthy subjects were served as a control group. Results(The plasma) homocysteine levels in group 1,group 2 and the control group were(14.87?5.22),(13.25?5.45) and((7.52)?1.73) ?mol/L,respectively.The plasma homocysteine level was significantly higher in group 1 and group 2 than in the control group(P

Maternal Plasma Homocysteine Levels and Pregnancy Outcomes / 대한지역사회영양학회지

Elevated maternal plasma homocysteine concentrations have been associated with adverse pregnancy outcomes, including birth defects, low birth weight, preeclampsia, spontaneous abortion, placental abruption, and other maternal or fetal complications. The purpose of this study was to assess the maternal plasma homocysteine level during pregnancy and to investigate the relationship between the plasma homocysteine concentrations and pregnancy outcomes. Venous blood samples were drawn from 82 pregnant women who were grouped with gestational age, 1st trimester (n = 26), 2nd trimester (n = 27) and 3rd trimester (n = 29). The concentration of plasma homocysteine was analyzed by HPLC, and pregnancy outcomes including gestational length, maternal weight gain, infant birth weight, and Apgar score were collected with the medical records of the pregnant women. The levels of plasma homocysteine of the pregnant women at the 1st, 2nd, and 3rd trimester were 5.7 +/- 3.7 micronmol/L, 5.6 +/- 4.1 micronmol/L and 7.0 +/- 4.5 micronmol/L, respectively, which had not showed any significant difference. The result of this study showed that in case of the pregnant women at the 1st trimester, the maternal plasma homocysteine level of the pregnant women whose gestational length was less than 38 weeks was significantly high (p < 0.01) compared to that of the pregnants whose gestational length was more than 38 weeks. And also, the level of homocysteine of the pregnant women at the 2nd trimester was significantly low when the maternal weight gain was high (p < 0.05). These findings suggest that maternal plasma homocysteine level at early stage of gestation will be a predicter of gestational length and maternal weight gain.

Effect of Endurance Training on the Plasma Homocysteine and B Vitamin Levels in Male Adolescent Field Hockey Players

Elevated plasma homocysteine is an independent risk factor for the development of cardiovascular disease. Exercise is generally believed to reduce the plasma homocysteine levels and therefore, being beneficial for cardiovascular disease(CVD). However, there is a possibility that athletes undergoing strenuous training and competition which increase oxidative stress may suffer from increased plasma homocysteine levels. The purpose of this study was to investigate the influence of endurance training on the plasma concentrations of B vitamins and homocysteine in 23 male adolescent field hockey players. Data collection and blood sampling was performed during the training period and non-training period. Following the training period, significant changes in energy and vitamin B6 intakes were observed in these subjects. Plasma vitamin B2, pyridoxal phosphate (PLP) and homocysteine levels were significantly higher during the training period than non-training period, whereas no difference was observed in plasma folate and vitamin B12 levels. Positive correlation was observed between plasma folate and folic acid intakes. When energy, B vitamin intakes were adjusted there was a significant negative correlation between plasma homocysteine levels and plasma riboflavin, folate and vitamin B12 levels. In conclusion, it is suggested that athletes with oxidative stress by strenuous exercise may need B vitamins since riboflavin, folic acid and vitamin B12 were shown to be negatively correlated with plasma homocysteine in athletes during the training period.