Effect of Organic Nutrients and Bio Fertilizers on Soil Parameters and Nutritional Content of Amaranth

A field experiment was conducted to study the effect of organic nutrients and bio fertilizers on soil parameters and nutritional content of amaranth. Eleven treatments were taken in three replications and the experiment was conducted in a randomized block design (RBD).The treatments were T1:RDF (60:30:20 kg NPK/ha), T2:Vermicompost @ 2 t/ha, T3:Vermicompost @ 5 t/ha, T4:FYM 10 t/ha, T5:FYM 20t/ha, T6:Poultry manure 3t/ha, T7:Poultry manure 5 t/ha, T8:Vermicompost @ 2 t/ha+PSB+Azospirillum@ 5 kg/ha each, T9 :FYM @ 10t/ha+ PSB+Azospirillum@ 5 kg/ha each, T10:Poultry manure 3 t/ha+ PSB+Azospirillum@ 5 kg/ha each and T11: Untreated control. The highest vitamin C (147.8 mg/100 gm) and crude fibre content (1.48%) was observed in the treatment T8 and T4, respectively. Maximum calcium (8.36%) and iron (0.21%) content was observed in the treatment T8. The treatment T10 recorded the highest mean performance for PH, organic carbon, available nitrogen and potash content in the soil analyzed after harvest of the crop.

Effect of Organic Manure and Zinc on Growth, Yield Attributes and Economics of Maize

A field experiment was conducted during Rabi 2022 season at Crop Research Farm, Department of Agronomy, Naini Agriculture Institute, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, Uttar Pradesh, to study the response of organic manure and soil application of zinc on the growth, yield attributes and economics of maize. The treatment consists of three level of zinc sulphate (2, 4 and 6 kg/ha) vermicompost (12 t/ha), poultry manure (4 t/ha) and control. The experiment was laid out in Randomized Block Design (RBD) with ten treatment and replicated thrice. The soil of the experimental field was sandy loam with pH (8.0), EC (0.56 ds/m), organic carbon (0.62%), available N (225 kg/ha), available P (38.2 kg/ha), and available K (240.7 kg/ha). The results showed that application of 50% poultry manure and 50% vermicompost along with zinc 6kg/ha recorded significantly higher plant height (208.33 cm), higher dry weight (89.90 g), number of cobs/plants (2.93), cob length (29.80 cm), number of seeds/cob (414.70). Maximum gross returns (254310.40 INR/ha), net returns (173950.40 INR/ha) and Benefit Cost (B.C) ratio (2.16).

Influence of Seaweed Sap and Organic Manures on Growth and Yield of Baby Corn

A field experiment was carried out during Rabi 2022 season at Crop Research Farm, Department of Agronomy, Naini Agriculture Institute, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, Uttar Pradesh, to study the response of Seaweed Sap and Organic Manures on Growth and Yield of Baby Corn. The treatments consists of three level of Seaweed sap (5%, 7.5% and 10%), Organic manures (FYM, Poultry manure, Vermicompost) and control. The experiment was laid out in Randomized Block Design (RBD) with ten treatments and replicated thrice. The soil of the experimental field was sandy loam with pH (8.0), EC (0.56 ds/m), Organic Carbon (0.62%), Available N (225 kg/ha), Available P (38.2 kg/ha), and Available K (240.7 kg/ha). The results showed that application of Seaweed sap (10%) + Vermicompost – 8.5t/ha recorded significantly higher plant height (167.58 cm), maximum dry weight (98.48 g), No. of cobs/plant (2.47), cob length (21.40 cm), Cob weight with husk (49.84 g/cob), Cob yield (11.03 t/ha) and stover yield (35.33 t/ha). Maximum gross returns (281216.67 INR/ha), net returns (216726.67 INR/ha) and Benefit Cost (B.C) ratio (3.36).

Response of NPK and Organic Manures on Growth, and Root, Yield of Carrot (Daucus carota L.) cv. Nantes

A trial was conducted at the Horticulture Research Farm (HRF), Department of Horticulture, Naini Agricultural Institute, SHUATS, Prayagraj, Uttar Pradesh during winter 2022 to study the “Response of NPK and Organic Manures on Growth and Root, Yield of Carrot (Daucus carota L.) cv. Nantes”. Results revealed that treatment T2 100% NPK emerged as superior over all other treatment combination in terms of Plant height 59.05 cm, Fresh weight of leaves per plant 137.18, Root yield per plot 4.30 kg, Yield 426.40 quintal/ha-1 T3 75%NPK + FYM number of leaves 19.88,T5 75% NPK + PM Fresh weight of plant 132.04 gm, Length of root 23.75 cm, Diameter of root 5.13 cm T7 50% NPK + VC 116.98 gm Showed higher B:C ratio (5.67) compared with the other treatments under Prayagraj Argo-Climatic conditions.

Effect of Poultry Manure, Vermicompost and Boron on Growth and Yield of Maize

A field experiment titled “Effect of Poultry Manure, Vermicompost and Boron on Growth and Yield of Maize” (Zea mays L.) was conducted during Rabi 2022 at Crop Research Farm. Department of Agronomy, Naini Agriculture Institute SHUATS, Prayagraj Uttar Pradesh. The experiment was laid out in Randomized Block Design with ten treatments which are replicated thrice. Results obtained that combined application of Poultry Manure 50% (1t/ha) along with Vermicompost 50% (2 t/ha) and Boron – 5% (Treatment) significantly increased higher plant height (234.45 cm), plant dry weight (135.84 g/plant) and also yield attributes like effective Number cobs per plant (2.00), Number of Seeds per cob (302.67), Seed index (24.69 g), Grain yield (6.73t/ha), stover yield (15.20 t/ha) and Harvest index (30.68%).The economics viz., maximum gross returns (INR/ha 39,832.43), net returns (INR/ha 84,600.00) and benefit cost ratio (2.48) was also recorded in treatment 9 [Poultry Manure 50% (1t/ha) + Vermicompost 50% (2 t/ha) + Boron – 5%)].

Effect of Organic Manure and Inorganic Fertilizer on Growth and Root Yield of Beetroot (Beta vulgaris L.)

Beetroot cultivation is benefited from the use of both organic and inorganic fertilizers. Organic fertilizers such as compost or manure improve soil fertility, enhance nutrient availability, and promote microbial activity. Inorganic fertilizers provide precise nutrient ratios and are readily available for immediate plant uptake. Combining organic and inorganic fertilizers ensures a balanced nutrient supply, promoting healthy growth, improved yield, and nutrient-rich beetroot production. The present investigation was carried out at Horticultural Research Farm (HRF), Department of Horticulture, Naini Agricultural Institute, SHUATS, Prayagraj, Uttar Pradesh during the Rabi-2022 with a view to identify the best treatment combination that performed in the Prayagraj region. The treatments comprised of T1 (Recommended dose of fertilizers (RDF)), T2 (75% (RDF) +25% (FYM)), T3 (75% (RDF) +25% (Vermicompost)), T4 (75% (RDF) +25% (Poultry Manure)), T5 (50% (RDF) + 50% (FYM)), T6 (50%(RDF) +50% (Vermicompost)), T7 (50%(RDF) +50% (Poultry Manure)), T8 (25% (RDF) + 75% (FYM)), T9 (25% (RDF) +75% (Vermicompost)), T10 (25% (RDF) +75% (Poultry Manure)), T11 (75% (RDF) + 12.5% (Poultry Manure) + 12.5% (Vermicompost)), T12 (50% (RDF) +25% (FYM) +25 % (Poultry Manure)) and T13 (25% (RDF)+ 50% (FYM)+ 25% (Vermicompost)). From the above experimental finding it is concluded that the treatment T11 (75% (RDF) + 12.5% (Poultry Manure) + 12.5% (Vermicompost)) was found to be best in the terms of growth and yield among different treatment combinations of Beet root.

Effect of Organic and Inorganic Fertilizer on Vegetative Growth Parameter of Lilium (Lilium Longiflorum) cv. Pavia under Shade Net Condition

The present investigation was under taken in the Department of Horticulture, Naini Agricultural Institute, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, (Allahabad). During winter season of 2020-2021, 2021-2022 and Pooled. The experiment was layout in Randomized Block Design (RBD) with 13 treatments and each treatment replicated thrice. The treatments consist of different combinations of inorganic fertilizers (N, P and K) and organic fertilizers (FYM, Vermicompost and Poultry Manure) and control (No fertilizers and manures). The treatment T5 50% RDF + FYM 14 t ha-1 was found the statistically significant compared to other treatment combination, which recorded highest plant height (23.68, 57.11 and 76.41 cm), spread area (8.19 cm2, 15.54 cm2 and 18.47 cm2), Number of leaves (36.63, 57.11 and 76.41), Leaf area (15.03 cm2, 15.63 cm2 and 15.33 cm2), Chlorophyll content (66.11 mg, 65.60 mg and 65.86 mg), followed by T9 25% RDF + Vermicompost 6.9 t ha-1 and lowest yield was obtained from T0 (control).

The effects on the root and plant development of soybean of organic fertilizer applications

Organic fertilizers increase soil aeration, water and nutrient holding capacity, improve seed germination and encourage the production of thicker roots, increase the yield and quality of the crop. This study was aimed to determine the effects of organic fertilizer (vermicompost, sheep manure, cattle manure, poultry manure and inorganic fertilizer as control) applications on the root and plant development of soybean during two growth stages, namely third trifoliate stage (V3) and beginning of flowering of the plants (R1) in the pot condition. The experiment was established with a two-factorial randomized plot design with four replications. The fertilizer applications had a significant effect (p<0.01) on the V3 and R1 growth stages of soybean. The highest plant height (36.78 cm) and root length (41.18 cm) were achieved with vermicompost, and the lowest plant height (21.73 cm) was recorded with inorganic fertilizer. The number of nodules was highest for vermicompost and cattle manure applications (17.16 plant-1 and 16.76 plant-1, respectively), and lowest for poultry manure (0.22 plant-1). In the R1 growth stage, poultry manure produced the highest biological fresh and dry weights, root dry weight at 25.08 g plant-1 and 5.67 g plant-1, 3.99 g plant-1 respectively. As a result, it was demonstrated that vermicompost and poultry manure applications promote strong root and plant development due to high organic matter and phosphorus and can be used successfully without inorganic fertilize application in soybean farming.

Environmental pollution and health hazards caused by animal agriculture manure / 环境与职业医学

The rapid growth of modern animal husbandry in China has supplied meat, eggs, and milk for Chinese residents, meanwhile the rapid transformation to intensive husbandry operations has also produced a large quantity of livestock and poultry manure. However, due to the failure of integration of animal husbandry and crop plantation, most of the livestock and poultry manure has not been treated and utilized, which may cause serious environmental pollution and public health risks. Antibiotics, heavy metals, pathogens, and other pollutants contained in livestock and poultry manure can enter farming soil, surface water, and groundwater during the transportation, storage, processing, and utilization of livestock and poultry manure, contaminate food, and pose direct and indirect risks to human health. Based on published statistics and papers, this article systematically analyzed the main pollutants discharged from livestock and poultry manure, their impacts on the environment, as well as the potential health risks by exposure to these pollutants. Recommendations on how to scientifically utilize animal manure were proposed, aiming to better protect human health.

Agronomic efficiency and profitability of cotton on integrated use of phosphorus and plant microbes / Eficiência e rentabilidade agronômica do algodão no uso integrado de fósforo e micróbios vegetais

Cotton crop, plays a significant role in Pakistan's economy by ruling a prominent place in edible oil and local textile industry. Phosphorus (P) inaccessibility and deficiency of soil organic matter are the key restraints for low crop productivity in cotton. Therefore, a two years field study was designed during 2014-15, to explore the influence of phosphate solubilizing bacteria (PSB), farmyard manure (FYM), poultry manure (PM) and inanimate sources of P on various physiological, growth, yield and quality parameters of cotton crop at CCRI Multan. Field responses of seeds inoculated with two distinctive phosphate solubilizing bacteria (PSB) strains viz. S0 = control, S1 =strain-1, S2 = strain-2 and eight organic, inorganic P sources viz., P0= control, P1 = 80 kg ha-1 P from inorganic source, P2 = 80 kg ha-1 P from FYM, P3 = 80 kg ha-1 P from PM, P4 = 40 kg ha-1 P from FYM + 40 kg ha-1 P from inorganic source, P5 = 40 kg ha-1 P from PM + 40 kg ha-1 P from inorganic source, P6 = 80 kg ha-1 P from FYM + 40 kg ha-1 P from inorganic source, P7 = 80 kg ha-1 P from PM + 40 kg ha-1 P from inorganic source and P8 = 40 kg ha-1 P from FYM + 40 kg ha-1 P from PM were evaluated. Results revealed that inoculation of seeds with PSB and collective use of inorganic and organic sources of P had considerably increased the yield contributing attributes in cotton. However, the treatment P7 (80 kg P ha-1 from PM + 40 kg P ha-1 from inorganic source) in coincidence with seeds inoculated with PSB (S1) produced taller plant, maximum boll weight, significantly higher LAI and CGR. Significantly higher seed cotton yield, lint yield, fiber length and maximum BCR of 1.95 and 1.81 was also obtained from the P7 treatment during both crop-growing seasons. In conclusion, combined use of 80 kg P ha-1 from PM + 40 kg P ha-1 from inorganic source and cotton seeds inoculated with strain-1 improved phosphorus uptake ensuing in greater consumption of photo-assimilates for maximum growth and yield.

A safra de algodão, desempenha um papel significativo na economia do Paquistão, ao ocupar um lugar de destaque no óleo comestível e na indústria têxtil local. A inacessibilidade e a deficiência de fósforo (P) da matéria orgânica do solo são as principais restrições para a baixa produtividade das culturas em algodão. Portanto, um estudo de campo de dois anos foi desenvolvido durante 2014-15, para explorar a influência de bactérias solubilizantes de fosfato (PSB), esterco de capoeira (FYM), esterco de aves (PM) e fontes inanimadas de P sobre vários fatores fisiológicos, crescimento, rendimento e parâmetros de qualidade da cultura do algodão no CCRI Multan. Respostas de campo de sementes inoculadas com duas cepas distintas de bactérias solubilizantes de fosfato (PSB) viz. S0 = controle, S1 = cepa-1, S2 = cepa-2 e oito fontes orgânicas de P inorgânicas viz., P0 = controle, P1 = 80 kg ha-1 P de fonte inorgânica, P2 = 80 kg ha-1 P de FYM, P3 = 80 kg ha-1 P de PM, P4 = 40 kg ha-1 P de FYM + 40 kg ha-1 P de fonte inorgânica, P5 = 40 kg ha-1 P de PM + 40 kg ha-1 P de fonte inorgânica, P6 = 80 kg ha-1 P de FYM + 40 kg ha-1 P de fonte inorgânica, P7 = 80 kg ha-1 P de PM + 40 kg ha-1 P de fonte inorgânica e P8 = 40 Foram avaliados kg ha-1 P da FYM + 40 kg ha-1 P da PM. Os resultados revelaram que a inoculação de sementes com PSB e o uso coletivo de fontes inorgânicas e orgânicas de P aumentaram consideravelmente os atributos que contribuem para a produtividade no algodão. No entanto, o tratamento P7 (80 kg P ha-1 da PM + 40 kg P ha-1 da fonte inorgânica) em coincidência com sementes inoculadas com PSB (S1) produziu planta mais alta, peso máximo de cápsula, IAF e CGR significativamente maiores. Produtividade significativamente maior do algodão, rendimento de fiapos, comprimento da fibra e BCR máximo de 1,95 e 1,81 também foi obtida a partir do tratamento P7 durante as duas épocas de cultivo. Em conclusão, o uso combinado de 80 kg P ha-1 de PM + 40 kg P ha-1 de fonte inorgânica e sementes de algodão inoculadas com a cepa-1 melhoraram a captação de fósforo, resultando em maior consumo de foto assimilados para obter crescimento e produtividade máximos.

Epidemiological determinants of Chrysomya (Diptera: calliphoridae) infestation in layer farms of Minas Gerais, Brazil / Determinantes epidemiológicos da infestação por Chrysomya (Diptera: Calliphoridae) em granjas avícolas de postura de Minas Gerais, Brasil

Aspects related to the epidemiology of Diptera belonging to genus Chrysomya were studied in order to determine predisposing factors for their occurrence in commercial laying hens farms from the state of Minas Gerais, Brazil. An observational, analytical and sectional survey was conducted to verify the main epidemiological determinants of infestations by Chrysomya spp. Forty-three farms were visited in 2012 and these 13 (30,23%) were infested by Chrysomya putoria, 4 (9,30%) by Chrysomya megacephala and 2 (4,65%) by Chrysomya albiceps and in 2 farms (4,65%) was not possible to identify the species of Chrysomya. The epidemiological determinants for the occurrence of infestations by Chrysomya spp. were investigated using multiple logistic regression models. Housing clustering type and its conditions were important factors involved in epidemiology of these species (OR=5.05, 95% CI = 2.66 to 9.58, p = 0.000). It was also observed that the increase of the humidity of manure leads to increase chances of infestations (OR = 1.81, 95% CI = 1.36 to 2.40, p = 0.000). The presence of the beetle "lesser mealworm" (Alphitobiusdiaperinus) (OR = 0.15, 95% CI = 0.067 to 0.36, p = 0.000) was characterized as a protective factor against infestations, probably due to predation behavior of the beetle. Some regions of the state were characterized as lower risk areas for Chrysomya spp. infestation as compared to other regions. Management measures, such as constant monitoring of water leaks on manure, adequate composting of dead poultry and periodic removal of manure could assist in decreasing the presence of Chrysomya spp. inside the laying houses.

Aspectos relacionados à epidemiologia das infestações por dípteros do gênero Chrysomyaforam estudados com o objetivo de determinar os fatores predisponentes para sua ocorrência em granjas comerciais de postura do estado de Minas Gerais, Brasil. Foi realizado um estudo observacional, analítico e seccional para verificar os principais determinantes epidemiológicos das infestações por Chrysomya spp. Foram visitadas 43 granjas avícolas em 2012 e dessas 13 (30,23%) apresentaram infestação por Chrysomya putoria, 4 (9,30%) por Chrysomya megacephala, 2 (4,65%) por Chrysomya albiceps e em 2 granjas (4,65%) não foi possível identificar as espécies de Chrysomya. A verificação dos determinantes epidemiológicos foi realizada utilizando modelos de regressão logística múltipla. Diferenças entre os núcleos das granjas avícolas e que não foram avaliadas neste estudo, apresentaram-se importantes na epidemiologia destas moscas (OR=5.05 95% IC = 2.66 a 9.58, p = 0.000). Observou-se também que o aumento na umidade do esterco dos aviários aumenta as chances de ocorrência de infestações (OR = 1.81, 95% IC = 1.36 a 2.40, p = 0.000). A presença de coleópteros popularmente conhecidos como "Cascudinhos", Alphitobiusdiaperinus (OR = 0.15, 95% IC = 0.067 a 0.36, p = 0.000), foi caracterizada como um fator protetor contra infestações, provavelmente devido ao comportamento predador deste coleóptero. Algumas regiões do estado apresentaram menores chances de ocorrência de infestações por Chrysomya spp. quando comparadas a outras regiões. Algumas medidas de manejo, como monitoramento constante de vazamentos de água no esterco, compostagem adequada de aves mortas e remoção periódica do esterco, poderiam auxiliar na diminuição da presença de Chrysomya spp. no interior dos galpões de postura.

Responses of Soil Properties and Crop Productivity to Peat- Fertilizers in Russia

In the present study, four fractions were prepared from leaves extract of Atriplex nummularia. This study aimed to determineTPP (total polyphenols), FLV (flavonoids) and CT (condensed tannins) on one hand and on the other hand, to evaluate theantiradical activity of the hydroalcoholic and aqueous fractions by four different methods including: DPPH test (2,2-diphenyl-1-picrylhydrazil), FRAP test (antioxidant activity by the iron reduction method), BCB test (β-carotene bleaching),and TAC test (antioxidant capacity) total) by the phosphomolybdate method. High levels of total polyphenols and condensedtannins were found in F/DieEA (23,437 ± 0.251mgGEA/gE), and F/DcmEA (17,251 ± 0.354mgCE/gE), respectively.Therefore, flavonoid levels F/DieEA and F/DcmEA were almost identical (9.515 ± 0.277 mgQE/gE), (9.238 ± 0.119mgQE/gE). The F/DcmEA showed an interesting and significant antioxidant activity in all tests as the other F/DieEA, F/nBUOH, F/aqueous represented the activity of trapping the free radical DPPH of C50 = (3.073 ± 0.088mg/ml), a reducingpower of iron (433.55 ± 24.36mgQE/gE), and a total antioxidant capacity of the Mo (VI) reduction test of (20.28 ±1.96mgEAG/gE), and the determination of IC50 of β-carotene bleaching (0.92 ± 0.0175mg/ml).

Composted slaughterhouse sludge as a substitute for chemical fertilizers in the cultures of lettuce (Lactuca sativa L.) and radish (Raphanus sativus L.)

Abstract Flotation sludge (FS) is produced in huge amounts at slaughterhouses in western Santa Catarina, Brazil. This waste is rich in plant nutrients and a valuable resource for soil amendments. Five FS composts were tested as a replacement for chemical fertilizers (QF), namely T1 (75% poultry manure (PM) and 25% sawdust (SD)); T2 (50% PM and 50% SD); T3 (25% PM and 75% SD); T4 (100% PM and 0% SD); and T5 (0% PM x 100% SD). For lettuce plants, treatments containing composted FS resulted in an increased number of leaves, leaf area and leaf fresh weight (LFW). T1 presented the best results with increases of 1.4 fold in LFW compared to plants supplemented with QF. T2 was the most effective treatment for radish with the best results of root fresh weight and root diameter. Although T4 had the highest nitrogen content, it did not present the best results in growth performance for lettuce or radish. The presence of higher proportions of SD in composts (25% for lettuce and 50% for radish) improved the physical characteristics of the soil and proved to be a more balanced compost.

Efecto de la gallinaza sobre Azotobacter sp., Azospirillum sp. y hongos micorrízicos arbusculares en un cultivo de cebolla (Allium fistulosum) / Effect of poultry manure on Azotobacter sp., Azospirillum sp. and arbuscular mycorrhizal fungi in anion culture (A. fistulosum) / Efeito do esterco de galinha fresco sobre Azotobacter sp., Azospirillum sp. e fungos micorrízicos arbusculares na cultura da cebola (A. fistulosum)

Resumen La gallinaza fresca, debido a su rápida disponibilidad está siendo ampliamente utilizada como fuente exclusiva de nutrientes, especialmente nitrógeno (N), en el cultivo de cebolla (Allium fistulosum), sin embargo, problemas de toxicidad por sales y acumulación de metales pesados en plantas han sido detectados. El objetivo de este trabajo fue estimar el efecto de la gallinaza fresca y compostada sobre las bacterias Azotobacter sp. y Azospirillum sp., así como en el número de esporas de hongos micorrízicos arbusculares (HMA), en el cultivo de cebolla. En el experimento se utilizó un diseño de bloques al azar con cuatro tratamientos y tres réplicas a partir de requerimientos en fertilización del cultivo y el análisis de suelos. Los tratamientos fueron: T1-Gallinaza fresca + Fertilizante foliar líquido quelatado-(14 Ton/ha=400 gr/ sitio gallinaza fresca + complejo biofertilizante a base de N, P y aminoácidos-1 L/ha.), T2-Gallinaza compostada + Fertilizante foliar líquido quelatado-14 Ton/ha=400 gr/sitio gallinaza fresca + (complejo biofertilizante a base de N, P y aminoácidos-1 L/ha.), T3-Fertilizante de síntesis química: fertirrigación con complejo de fertilizante 20-20-20 + elementos menores (10 g/sitio=350 kg/ha) y T4-Fertilizante de síntesis química: Aplicación en el suelo con complejo de fertilizante 20-20-20+elementos menores (20 g/sitio=700 kg/ha.). Los resultados obtenidos indican que la gallinaza cruda tiene un impacto positivo en el rendimiento del cultivo de cebolla y la esporulación de HMA (91 esporas por gamo de solo), un efecto negativo en Azotobacter sp. con mejor desarrollo en la gallinaza compostada (1,0 x107 UFC) y en Azospirillum sp. sin respuesta relevante, sin embargo fue más abundante que Azotobacter sp.en cada uno de los tratamientos.

Abstract Poultry manure application is highly used as exclusive nutrient sources, especially N, in anion culture (A. fistulosum), However there has been detected toxicity problems by salts and heavy metals in plants. The aim of this study was to estimate the effect of fresh poultry manure and composted on Azotobacter sp., Azospirillum sp. and arbuscular mycorrhizal fungi (AMF). The treatments were performed under completely randomized design with 4 treatments and 3 replications according to fertilization requirements and soil analysis. Treatments were formed as follows: Tl-Poultry manure + Foliar liquid fertilizer quelated-14 ton.ha-1=400 grsite-1 poultry manure + complex biofertilizer of N, P and aminoacids-1 L.ha-1, T2-Composted poultry manure + Foliar liquid fertilizer quelated-14 ton.ha-1=400 grsite-1 poultry manure + complex biofertilizer of N, P and aminoacids-1 L.ha-1, T3-Chemical fertilizer: fertirrigation with complex biofertilizer 20-20-20 + minor elements (l0 grsite-1 =350 kg ha-1) and T4-Chemical fertilizer: Application in the soil with complex biofertilizer 20-20-20 + minor elements (20 kg ha-1=700 kg ha-1.). We observed that anion culture and AMF spores (91 spores by 1g soil) have a significant respond to poultry manure. Azotobacter sp. was negatively affected by poultry manure but positively influenced by composted poultry manure (l.0 xl07 CFU). We no observed a relevant respond of Azospirillum sp., although, it was more abundant than Azotobacter sp. in all treatments.

Resumo O esterco de galinha fresco, pela sua rápida disponibilidade é utilizada como fornecedor de nutrientes, especialmente N, na cultura da cebola (A. fistulosum), No entanto, problemas de toxicidade por sais e acumulação de metais pesados em plantas tem sido detectado. O objetivo deste trabalho foi estimar o efeito do esterco de galinha fresco e decomposto sobre as bactérias Azotobacter sp. e Azospirillum sp. e o número de esporos de fungos micorrízicos arbusculares (FMA) na cultura da cebola. O delineamento experimental foi de blocos ao acaso, com quatro tratamento e três repetições, segundo os requerimentos nutricionais da cultura e a análise de solo. Os tratamentos foram os seguintes: Tl-Cama aviaria fresca + fertilizante foliar líquido quelatado- (14 Ton/ha=400 gr/local-cama aviaria fresca + complexo biofertilizante baseado em N, P y aminoácidos-1 L/ha.), T2-Cama aviaria + fertilizante foliar líquido quelatado-14 Ton/ha=400 gr/local-Cama aviaria + (complexo biofertilizante baseado em N, P y aminoácidos-1 L/ha.), T3-Fertilizante de síntese química: fertirrigação com complexo de fertilizante 20-20-20 + elementos menores (10 g/local=350 kg/ha) y T4-Fertilizante de síntese química: Aplicação no solo com complexo de fertilizante 20-20-20 + elementos menores (20 g/local=700 kg/ha.).Os resultados aqui obtidos mostram efeito positivo do esterco de galinha no rendimento da cultura da cebola e da esporulação dos HMA (91 esporos por gama de solo), efeito negativo em Azotobacter sp., se desenvolvendo melhor no esterco de galinha decomposta (1,0 x107 UFC) e sem resposta relevante em Azospirillum sp., embora tenha sido mais abundante do que Azotobacter sp. em todos os tratamentos.

Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry Analysis of 16 Kinds of Residual Antibiotics in Chicken Manure / 分析化学

A sensitive and effective method for determination of 16 kinds of antibiotics, including tetracycline, sulfonamide, fluoroquinolone and macrolide, in livestock and poultry manure using solid phase extraction-ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established.Aiming at the chemical properties and sample impurities of the target, the parameters such as mass spectrum conditions, types of extraction and ultrasonic power were optimized.Finally, the samples were extracted with 50% acetonitrile in phosphate buffer solution (pH =4) for three times, followed by ultrasonic steaming, centrifugal and rotary, dilution, and purified by SAX-HLB.After sample loading, the solid phase was washed with 10 mL of methanol-acetone (80∶20, V/V), evaporated to near dryness at 35℃, and then re-dissolved and vortex mixed in 1 mL of 0.1% formic acid∶methanol (1∶1, V/V).The extracts were analyzed with UPLC-MS/MS and calculated by external standard method based on the monitored product ion.The results indicated that the average spiked recoveries of tetracycline, sulfonamide, fluoroquinolone and macrolide in manure were 56.4%-94.6% with relative standard deviations (RSDs) of 2.6%-19.8%, the LODs (S/N=3) were 0.01-2.50 μg/kg, and the LOQs (S/N=10) were 0.05-7.90 μg/kg.The method was simple with high stability, high sensitivity and good reproducibility, and suitable for the simultaneously determination of many antibiotics in animal and poultry manure.

Agronomic efficiency and profitability of cotton on integrated use of phosphorus and plant microbes

Abstract Cotton crop, plays a significant role in Pakistans economy by ruling a prominent place in edible oil and local textile industry. Phosphorus (P) inaccessibility and deficiency of soil organic matter are the key restraints for low crop productivity in cotton. Therefore, a two years field study was designed during 2014-15, to explore the influence of phosphate solubilizing bacteria (PSB), farmyard manure (FYM), poultry manure (PM) and inanimate sources of P on various physiological, growth, yield and quality parameters of cotton crop at CCRI Multan. Field responses of seeds inoculated with two distinctive phosphate solubilizing bacteria (PSB) strains viz. S0 = control, S1 =strain-1, S2 = strain-2 and eight organic, inorganic P sources viz., P0= control, P1 = 80 kg ha-1 P from inorganic source, P2 = 80 kg ha-1 P from FYM, P3 = 80 kg ha-1 P from PM, P4 = 40 kg ha-1 P from FYM + 40 kg ha-1 P from inorganic source, P5 = 40 kg ha-1 P from PM + 40 kg ha-1 P from inorganic source, P6 = 80 kg ha-1 P from FYM + 40 kg ha-1 P from inorganic source, P7 = 80 kg ha-1 P from PM + 40 kg ha-1 P from inorganic source and P8 = 40 kg ha-1 P from FYM + 40 kg ha-1 P from PM were evaluated. Results revealed that inoculation of seeds with PSB and collective use of inorganic and organic sources of P had considerably increased the yield contributing attributes in cotton. However, the treatment P7 (80 kg P ha-1 from PM + 40 kg P ha-1 from inorganic source) in coincidence with seeds inoculated with PSB (S1) produced taller plant, maximum boll weight, significantly higher LAI and CGR. Significantly higher seed cotton yield, lint yield, fiber length and maximum BCR of 1.95 and 1.81 was also obtained from the P7 treatment during both crop-growing seasons. In conclusion, combined use of 80 kg P ha-1 from PM + 40 kg P ha-1 from inorganic source and cotton seeds inoculated with strain-1 improved phosphorus uptake ensuing in greater consumption of photo-assimilates for maximum growth and yield.

Resumo A safra de algodão, desempenha um papel significativo na economia do Paquistão, ao ocupar um lugar de destaque no óleo comestível e na indústria têxtil local. A inacessibilidade e a deficiência de fósforo (P) da matéria orgânica do solo são as principais restrições para a baixa produtividade das culturas em algodão. Portanto, um estudo de campo de dois anos foi desenvolvido durante 2014-15, para explorar a influência de bactérias solubilizantes de fosfato (PSB), esterco de capoeira (FYM), esterco de aves (PM) e fontes inanimadas de P sobre vários fatores fisiológicos, crescimento, rendimento e parâmetros de qualidade da cultura do algodão no CCRI Multan. Respostas de campo de sementes inoculadas com duas cepas distintas de bactérias solubilizantes de fosfato (PSB) viz. S0 = controle, S1 = cepa-1, S2 = cepa-2 e oito fontes orgânicas de P inorgânicas viz., P0 = controle, P1 = 80 kg ha-1 P de fonte inorgânica, P2 = 80 kg ha-1 P de FYM, P3 = 80 kg ha-1 P de PM, P4 = 40 kg ha-1 P de FYM + 40 kg ha-1 P de fonte inorgânica, P5 = 40 kg ha-1 P de PM + 40 kg ha-1 P de fonte inorgânica, P6 = 80 kg ha-1 P de FYM + 40 kg ha-1 P de fonte inorgânica, P7 = 80 kg ha-1 P de PM + 40 kg ha-1 P de fonte inorgânica e P8 = 40 Foram avaliados kg ha-1 P da FYM + 40 kg ha-1 P da PM. Os resultados revelaram que a inoculação de sementes com PSB e o uso coletivo de fontes inorgânicas e orgânicas de P aumentaram consideravelmente os atributos que contribuem para a produtividade no algodão. No entanto, o tratamento P7 (80 kg P ha-1 da PM + 40 kg P ha-1 da fonte inorgânica) em coincidência com sementes inoculadas com PSB (S1) produziu planta mais alta, peso máximo de cápsula, IAF e CGR significativamente maiores. Produtividade significativamente maior do algodão, rendimento de fiapos, comprimento da fibra e BCR máximo de 1,95 e 1,81 também foi obtida a partir do tratamento P7 durante as duas épocas de cultivo. Em conclusão, o uso combinado de 80 kg P ha-1 de PM + 40 kg P ha-1 de fonte inorgânica e sementes de algodão inoculadas com a cepa-1 melhoraram a captação de fósforo, resultando em maior consumo de foto assimilados para obter crescimento e produtividade máximos.

Poultry litter of wood shavings and/or sugarcane bagasse: animal performance and bed quality / Cama de viruta de madera y/o bagazo de caña de azúcar para pollo: desempeño animal y calidad de la cama / Cama composta por maravalha e/ou bagaço de cana de açúcar para frangos: desempenho animal e qualidade da cama

Background: litter provides comfort to animals while improving productive performance and carcass quality. Objective: this study evaluated broiler performance, incidence of contact dermatitis, and quality of poultry litter of wood shavings and/or sugarcane bagasse (SB) after five consecutive flocks. Methods: two thousand birds were raised in five consecutive flocks composed of 400 chicks each. The experiment was set in a completely randomized design with five treatments and four repetitions. The treatments consisted of substituting wood shavings with different levels of SB (0, 25, 50, 75, and 100%). Results: litter density, pH, dry matter content, total nitrogen, and phosphorus did not vary between treatments. However, substituting wood shavings with SB increased moisture retention capacity of the litter and ammonia production. Neither carcass lesions nor productive performance, with the exception of weight gain and feed conversion ratio in the fifth flock, differed between treatments. Conclusions: substituting 75% of wood shavings with SB maintained litter quality and improved poultry performance.

Antecedentes: la cama propicia el confort y mejora el desempeño productivo y la calidad de la canal de los pollos de engorde. Objetivo: el objetivo del presente estudio fue evaluar el desempeño productivo de pollos de engorde, la incidencia de dermatitis de contacto en la canal y la calidad de la cama de pollo compuesta de viruta de madera y/o bagazo de caña de azúcar (SB), usada en cinco lotes consecutivos. Métodos: dos mil aves fueron criadas en cinco lotes consecutivos, cada uno constituido por 400 pollitos, en un diseño completamente aleatorizado con cinco tratamientos y cuatro repeticiones. Los tratamientos consistieron en diferentes niveles de sustitución de viruta de madera con SB (0, 25, 50, 75 y 100%). Resultados: la densidad, pH y contenidos de materia seca, nitrógeno total y fósforo no variaron entre los tratamientos. Sin embargo, sustituir viruta de madera con SB aumentó la capacidad de retención de humedad y la cantidad de amoníaco volatilizado en el galpón. Ni las lesiones en la canal y ni el desempeño productivo, con excepción de la ganancia de peso y conversión alimentaria en el quinto lote, difirieron entre los tratamientos. Conclusiones: sustituir 75% de la viruta de madera con SB mantuvo la calidad de la cama y mejoró el desempeño productivo de las aves.

Antecedentes: cama de frango propicia conforto e melhora o desempenho produtivo e a qualidade da carcaça das aves. Objetivo: este estudo avaliou o desempenho produtivo de frangos de corte, a incidência de dermatite de contato na carcaça e a qualidade da cama de frango composta de maravalha e/ou bagaço de cana (SB), usada em cinco lotes consecutivos. Métodos: duas mil aves foram criadas em cinco lotes consecutivos, compostos de 400 pintinhos cada, em delineamento inteiramente casualizado com cinco tratamentos e quatro repetições. Os tratamentos consistiram de diferentes níveis de substituição de maravalha com BC (0, 25, 50, 75 e 100%). Resultados: densidade, pH e teores de matéria seca, nitrogênio total e fósforo não variaram entre os tratamentos. Entretanto, substituir maravalha com SB aumentou a capacidade de retenção de umidade e a quantidade de amônia volatilizada no galpão. Nem as lesões na carcaça e nem o desempenho produtivo, com exceção do ganho de peso e conversão alimentar no quinto lote, diferiram entre os tratamentos. Conclusões: substituir 75% da maravalha com SB manteve a qualidade da cama e melhorou o desempenho produtivo das aves.

Potencial de recuperação física de um latossolo vermelho, sob pastagem degradada, influenciado pela aplicação de cama de frango / Potencial of physical recovery of a red latosol, under degraded pasture, as affected by the apllication of chicken manure

A criação intensiva de frango gera uma grande quantidade de resíduos ricos em nutrientes cujo uso agrícola pode ser viabilizado pelos produtores, na adubação das culturas comerciais. Objetivou-se avaliar a influência da fertilização com cama de frango na recuperação física de um Latossolo. O experimento foi instalado em um Latossolo Vermelho Distrófico típico, textura média, sob pastagem degradada de Brachiaria decumbens Stapf. Os tratamentos estudados foram: 0, 1.200, 2.400, 4.800 kg ha-1 de cama-frango e 2.400 kg ha-1 de cama-frango + adubação mineral com 36 kg ha-1 N, 60 kg ha-1 K2O e 60 kg ha-1 de P2O5. As aplicações foram realizadas em janeiro de 2004 e o solo amostrado em duas épocas distintas (60 e 210 dias após aplicação), nas profundidades de 0-20 e 20-40 cm, para a caracterização dos atributos físicos: argila dispersa em água, grau de floculação, densidade do solo e porosidade total. O teor de argila dispersa em água decresceu com o aumento das doses de cama aplicadas e, conseqüentemente, houve um incremento no grau de floculação; houve uma pequena redução na densidade do solo e na porosidade total. Os atributos físicos do solo avaliados responderam com maior intensidade às aplicações de cama de frango nas doses variando de 2.666 a 3.750 kg ha-1.

Poultry facilities generate great amount of residues rich in nutrients, which may be used by farmers to fertilize their crops. The aim of this work was to evaluate the effect of the fertilization with chicken manure on the physical recovery of the structure of a Latosol. The experiment was carried out in a loamy typic dystrophic Red Latosol, at Brachiaria decumbens Stapf. degraded pastureland. The treatments were: 0; 1,200; 2,400; 4,800 kg ha-1 of chicken manure; and 2,400 kg ha-1 of chicken manure + chemical fertilizer with 36 kg ha-1 N; 60 kg ha-1 of K2O; and 60 kg ha-1 of P2O5. The treatments were applied in January 2004. Sixty and 210 days after, the soil was sampled at the 0-20 and 20-40 cm depths. The physical attributes measured were: water dispersible clay, flocculation index, bulk density and total porosity. The water dispersible clay decreased with the increase of the dosage of manure applied; there was an increase in the flocculation index as the manure doses increased. There was a small reduction in soil bulk density and total porosity with the application of manure. The soil physical attributes evaluated were greatly affected by applications of chicken manure in the dose range of 2,666 to 3,750 kg ha-1.