RESUMO
This study was designed to construct a "drug-core target-pathway" network of Erzhi Pill for hepatic injury treatment in an effort to explore the "multi-components, multi-targets, multi-pathways" mechanism. ADME/T calculation method was used to screen the active components of Erzhi Pill, and then predict the potential targets according to the reverse pharmacophore matching method. Biological information annotation databases (DAVID) was used to analyze the molecular function and biological process of the action targets. The Cytoscape software was used to construct the "ingredient-core target-pathway" network of Erzhi Pill for hepatic injury treatment. It was found that 39 major active ingredients of Erzhi Pill regulated 321 targets (HRAS, DCK, HSD17B1, UCK2, et al) and affected 51 pathways, such as insulin signaling pathway, FoxO signaling pathway, metabolic pathways and glycolysis/gluconeogenesis. The method revealed the action features of traditional Chinese medicine as multi-ingredients, multi-targets, multi-pathways, providing new clues for further basic study on the hepatic injury pharmacological mechanism of Erzhi Pill.
RESUMO
Objective: To study the therapeutical effect of Jiuwei Gantai Capsule (JWGT) on nonalcoholic fatty liver disease (NAFLD) of rats induced by high fat diet and its possible therapeutic mechanism. Methods: The experimental rats were divided into six groups: normal group, model group, silymarin group (0.05 g/kg), JWGT high-, mid- and low-dose groups (1.80, 0.90, and 0.45 g/kg). Except the normal group, NAFLD rats were fed with high fat diet for 10 weeks, those other groups were ig given relevant medicine from the week 5. All the rats were put to death after 10 weeks, the changes of body weight, liver weight, liver index, and hepatic histopathological morphology were observed. The high density lipoprotein cholesterol (HDL-C), free fatty acid (FFA), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in serum were collected for the contents detection. Total choesterol (TC), triacylglycerol (TG), superoxide dismutase (SOD), and malondialdehyde (MDA) in hepatic tissue were detected, too. Results: Compared with the model group, the content of FFA, AST, and ALT in serum of NAFLD rats were decreased (P < 0.05), while HDL-C was obviously improved (P < 0.05) in JWGT high- and mid-dose groups. The TC, TG, and MDA in the hepatic tissue were obviously decreased (P < 0.05), while the activity of SOD was significantly improved (P < 0.05). Meanwhile, the pathological images of hepatic tissue and classified graded method of steatosis were improved in NAFLD rats. Conclusion: JWGT has the regulation of lipid and protection of liver for NAFLD rats. Its therapeutic mechanism is partly related to regulating lipid metabolism and anti-oxidative damage.