A review on cell-based models of human liver disease in vitro / 生物医学工程学杂志

Unhealthy diet, habits and drug abuse cause a variety of liver diseases, including steatohepatitis, liver fibrosis, liver cirrhosis and liver cancer, which seriously affect human health. The fabrication of highly simulated cell models in vitro is important in the treatment of liver diseases and drug development. This article summarized the common strategies for the construction of liver pathology models

Detection and identification of non-stress protein biomarkers for nephroblastoma / 中华实用儿科临床杂志

Objective To test the differential proteomics by using proteomic technology of children diagnosed with nephroblastoma and healthy children,thereby for screening non-stress-related protein markers of Wilms' tumor.Methods The serum of children with Wilms' tumor,children with trauma in 1-3 hours and healthy children were collected in the First Affiliated Hospital of Zhengzhou University from May 2010 to May 2014.Then,the differential proteomics were screened and the interference of traumatic stress proteins in the process were eliminated by using proteomic technology of surfaced enhanced laser desorption/ionization time of flight mass spectroscopy (SELDI-TOF-MS),matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF-MS),high performance liquid chromatography(HPLC),and two dimensions-liquid chromatography-linear trap quadrupole-mass spectrometer(2D-LC-LTQ-MS),therefore the non-stress-related protein biomarkers of Wilms' tumor were determined.Results A mass-to-charge ratio 6630.58 Da protein or peptide was selected as tumor-specific marker.And there were no same or similar proteins in mass spectrometry of children with traumatic stress by SELDI-TOF-MS.Finally,by purification through HPLC and identification through MALDI-TOF-MS and 2D-LC-LTQ-MS,apolipoprotein CI(APO CI) was found to be the non-stress-related serum protein of Wilms' tumor.Conclusions It is identified that APO CI is an important serum protein biomarker of Wilms' tumor by using proteomic technology in eliminating the influences of interference factors of stress.Therefore,the results provide possibilities of further studies and investigating the mechanisms of the protein expression changes and early diagnosing the Wilms' tumor.

Proteomic analysis of the serum from patients with acute rejection after liver transplantation / 天津医药

Objective To investigate the protein markers that specifically expressed in patients with acute rejection (ACR) after liver transplantation, and to explore preliminarily the mechanisms. Methods Serum samples from three patients with pathologically confirmed ACR after liver transplantation in Tianjin First Central Hospital were collected as ACR group. Three serum samples from patients with normal liver function indicators after liver transplantation were collected as No-ACR group. And six serum samples from healthy examination were mixed with equal amount as healthy control group. Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) was employed to separate, screen and identify the differentially expressed proteins between three groups. KEGG and STRING software were applied to deeply analyze the data of three groups. Results A total of 88 differentially expressed proteins were found between ACR group and healthy control group. There were 39 differentially expressed proteins between No-ACR group and healthy control group. Ten differentially expressed proteins were acquired between ACR group and No-ACR group. Comparing 88 and 10 differentially expressed proteins, 9 proteins were the same. Among 88 differentially expressed proteins, 30 of them showed a direct interaction, and can be positioned in 13 signaling pathways based on KEGG and STRING software. Fourteen (46.67%) of the 30 proteins were located in the complement and coagulation cascade pathway. Among 39 differentially expressed proteins, which were detected between No-ACR group and control group, 10 proteins showed a direct interaction including 9 proteins concentrated in the complement and coagulation cascade pathway. Conclusion By proteomic analysis, nine differentially expressed proteins are obtained, which may be regarded as the candidate bio-markers for ACR early diagnosis after liver transplantation. The complement and coagulation cascades system is significantly adjusted after liver transplantation, indicating this pathway plays an important role in the occurrence of ACR.