Qualitative evaluation of Forsythia suspensa by HPLC-PDA fingerprint combined with UFLC-Q-TOF-MS qualitative identification / 中国中药杂志

The analysis of Forsythia suspensa was performed on Waters Symmetry C18 column( 4. 6 mm×250 mm,5 μm) and mobile phase was methanol( A)-0. 1% formic acid aqueous solution( B) with the elution gradient. Column temperature was maintained at 30℃,and the flow rate was 1. 0 m L·min-1 with detection wavelength 265 nm. The HPLC-PDA fingerprint of F. suspensa was optimized.Chemical constituents in F. suspensa were analyzed by UFLC-Q-TOF-MS in positive and negative ion mode. The quality of 48 batches of F. suspensa from different habitats,processing methods and specifications was evaluated by similarity evaluation and cluster analysis.The 18 common peaks were confirmed. The similarity of F. suspensa from different habitats was more than 0. 98,and 56 chemical constituents were identified. Different processing methods had great influence on the quality of F. suspensa. Compared with boiled and direct drying,the quality of F. suspensa processed by sun-drying was obviously decreased. The similarity was about 0. 58. Different specifications of F. suspensa also had obvious distinction,and the similarity was about 0. 78. The effective components of grown F. suspensa,such as forsythoside A and phillyrin,were significantly reduced. The results of cluster analysis were basically consistent with the results of similarity evaluation. The establishment of fingerprint and the recognition of chemical pattern of F. suspensa can provide a more comprehensive reference for the quality control of herbs.

Study on Components of Effective Parts Ⅰ in Zhou's Prescription of Anti-Human Lung Cancer on A549 Cells / 中国中医药信息杂志

Objective To study material basis of Zhou's prescription, components of effective parts Ⅰ in Zhou's prescription was gualitative and quantitative analyzed. Methods The components of effective part Ⅰ was identified by test-tube reaction and qualitative TLC method, the content of saponins and flavones was quantitative analyzed. Results Total content of the saponins and flavone are 1.07% and 0.85% in Zhou's Prescription. The purity of saponins and flavones in effective parts Ⅰ are 37.4% and 29.7%. Conclusion The main components of effective part Ⅰ in Zhou's Prescription were flavones and saponins.

Pharmacognostical Study on the Fruit and the Seed of Livistona chinensis / 中国药房

OBJECTIVE: To study the pharmacognosy of the fruit and the seed of Livistona chinensis.METHODS: The pharmacognosy of the fruit and the seed of L.chinensis was identified qualitatively and microscopically.RESULTS: The shape,the characteristics of the ectocarp,mesocarp and episperm,the stone cells of the power,the brown mass and the oil cells can all serve as characteristics for the identification of the pharmacognosy of the fruit and the seed of L.chinensis.CONCLUSION: The study provides scientific evidence for the identification of the pharmacognosy of the fruit and the seed of L.chinensis.

Qualitative Identification of 5 Kinds of Fritillaria / 中国药房

OBJECTIVE:To study the microscopic TLC characteristics of Fritillaria thunbergii,Bulbus Fritillariae Cirrhosae,Fritillaria hupehensis,Fritillaria ussuriensis and Bulbus Fritillariae Pollidiflorae.METHODS: The five kinds of Fritillaria(powder)was identified microscopically in accordance with Chinese Pharmacopeia,and two development systems were applied for the TCL identification of the five kinds of Fritillaria.RESULTS: The microscopic characteristics of the 5 kinds of Fritillaria were obtained.Both peimine and peiminine were noted in the TLC of Fritillaria thanbergii,Bulbus Fritillariae Cirrhosae,Fritillaria hupehensis and Fritillaria ussuriensis,but were not noted in the TLC of Bulbus Fritillariae Pollidiflorae,however,imperialine was noted in the TLC of Bulbus Fritillariae Pollidiflorae.CONCLUSION: The results serve as basis for the identification of 5 kinds of Fritillaria.

TLC Identification of Shenjin Huoxue Mixture / 中国药房

OBJECTIVE:To establish a method for qualitative identification of Shenjin huoxue mixture.METHODS:Ly-copodii japonicum,Paeonia lactiflora,Dipsacus asperoides and Radix et Rhizoma Glycyrrhizae in the preparation were identified by TLC.RESULTS:At the corresponding positions,the color of TLC spots of Paeonia lactiflora,Dipsacus asperoides and Radix et Rhizoma Glycyrrhizae in the test samples and in reference substances were identical.There was no interference form negative control.But no characteristic spots of Lycopodii japonicum in the sample were noted.CONCLUSION:The established TLC identification method of Paeonia lactiflora,Dipsacus asperoides and Radix et Rhizoma Glycyrrhizae is reproducible,specific,reliable and suitable for the quality control of Shenjin huoxue mixture.The absence of characteristic TLC spots of Lycopodii japonicum in the sample is suggestive of the necessity of improving the production technology of Shenjin huoxue mixture.

Identification of traditional Chinese medicine injection by FTIR combined with computer aided analysis / 中成药

AIM: To investigate a new method for identification of traditional Chinese medicine injection by FTIR. METHODS: For the steadiness of spectra, the factors of effecting spectrum's information quality were all investigated scientifically over the experiment procedures and instrumental setting, such as the preparation of samples, resolution ratio, scanning times, repeating scanning times, etc. The traditional Chinese medicine injections were used as the analytical samples such as Radix Isatidis, Rhizoma Chuanxiong, Flos Carthami, Radix Astragali and Herba Houttuyniae. RESULTS: Although all these original spectrums were similar at a certain degree, the FTIR combined with computer aided analysis, such as the cluster analysis and derivative spectrometry comparability calculation could be used to identify these injections. CONCLUSION: The method of identification by FTIR is non destructive testing, cheap, clean, fast, simple and convenient. The result indicates this method is suitable for establishing identification database of traditional Chinese medicine injections.

Fingerprints of Qingkailing Injection by HPLC/UV/MS/MS / 中成药

AIM: To develop a method of the HPLC/UV/MS/MS fingerprints of Qingkailing Injection(Radix Isatidis,Fructus Gardeniae,Flos Lonicerae Japonicae,etc.) and provide a novel conception for its quality control. METHODS: Separation was performed on Phenomenex Luna C_(18) analytical column(250 mm?4.6 mm,5 ?m).The mobile phases consisted of water containing 0.1% formic acid and acetonitrile was used as gradient elute.The flow rate was 1 mL/min.An Electrospray Ion Trap mass spectrometer was utilized for qualitative analysis and both positive and negative secondary ion scan mode were applied. RESULTS: Perfect fingerprints with excellent separation and reproducibility were obtained and 13 compounds were identified.Especially three isomers,including Neochlorogenic acid,Chlorogenic acid and Isochlorogenic acid,were differentiated successfully. CONCLUSION: The method is suitable for establishing the HPLC/MS/MS fingerprints of Qingkailing Injection and hence could be used as an effective and credible pattern for analyzing traditional Chinese medicine as well as its quality control.Moreover,based on this approch,further traditional Chinese medicine research can be performed.