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By reviewing the research history on quality comparison between wild and cultivated Chinese crude drugs， this paper systematically combed the relevant research reports since the 1950s， and summarized and analyzed the results of existing comparative studies， and found that the existing comparative research on the quality of wild and cultivated Chinese crude drugs were mainly focused on several aspects， including characteristics， microstructures， chemical compositions， pharmacodynamic effects， and genetic diversity. Among these， comparative studies of chemical compositions have been the dominant approach， with a particular emphasis on comparing the contents of index components. However， research on pharmacodynamic effects remained relatively limited. Due to various factors such as sample quantity， sample origin， growth period and cultivation methods， the differences in quality between wild and cultivated Chinese crude drugs vary significantly. In general， most wild Chinese crude drugs exhibited higher quality than cultivated products， with significant differences in their characteristics. The contents and proportions of some chemical components underwent noticeable changes， particularly with a marked increase in the proportion of primary metabolites after cultivation. The quality of cultivated Chinese crude drugs is closely related to the cultivation practices employed. Chinese crude drugs produced through wild nurturing， simulated wild planting， ecological cultivation， and other similar methods demonstrate quality levels comparable to those of wild Chinese crude drugs. Based on the analysis results， it is recommended to explicitly specify the cultivation practices and cultivation period of cultivated Chinese crude drugs in comparative studies of the quality between wild and cultivated Chinese crude drugs. Multiple technical approaches， including characteristics， microscopy， non-targeted metabolomics combined with quantitative analysis of differential components， and bioefficacy evaluation， should be employed to comprehensively assess the quality disparities between wild and cultivated Chinese crude drugs. Moreover， research efforts should be intensified to investigate the changes in pharmacodynamic effects resulting from differences in plant cell wall composition， primary metabolites， and secondary metabolites， in order to guide the production of high-quality Chinese crude drugs.
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Indigo Naturalis has a long history of medicinal use with particularity and complexity in its processing. Before the Ming dynasty,Indigo Naturalis was extracted from the top layer of zymotic fluid,called " purified Indigo Naturalis". In modern processing,the precipitate " crude Indigo Naturalis" is dried to produce Indigo Naturalis after impurity removal. The form of Indigo Naturalis slices has undergone significant changes in ancient and modern times. In view of this,the quality comparison between crude Indigo Naturalis and purified Indigo Naturalis was conducted in this study with modern analytical techniques. Firstly,chemical composition was analyzed with UPLC-Q-TOF-MS,and the chemical composition of scent with HS-SPME/GC-MS/MS. The content of indigo,indirubin,total ash,and water-soluble extract was determined as well as the inorganic composition in crude Indigo Naturalis and purified Indigo Naturalis. Then,their microscopic morphology was observed and the surface element composition was investigated. Finally,the antipyretic activities of crude Indigo Naturalis and purified Indigo Naturalis were compared in the fever rat model induced by lipopolysaccharide and 2,4-dinitrophenol. The results demonstrated that the purified Indigo Naturalis had a faster and more lasting antipyretic effect,while the crude Indigo Naturalis had almost no antipyretic effect. This study is of great significance to the research on processing technology of Indigo Naturalis and provides reference for the formulation of its quality standards,production specifications and calibration procedures.
Assuntos
Animais , Ratos , Índigo Carmim , Indigofera , Extratos Vegetais , Espectrometria de Massas em TandemRESUMO
OBJECTIVE: To investigate the similarities and differences of the quality of agarwood produced by agar-wit from China and Southeast Asian countries. METHODS: The sensory characteristic, identification item, extract, content determination, characteristic chromatogram and total chromone content of agarwood produced by agar-wit from 11 place of origins of China and Southeast Asian countries were mainly evaluated, according to the Chinese Pharmacopoeia (2015 edition). RESULTS: The morphological characteristics, TLC and characteristic chromatograms of 11 batches of agarwood produced by agar-wit were similar. Special structures such as included phloem, vessel and rays were observed in all samples. The extract and total chromone contents of agarwood produced by agar-wit from the six producing areas in China were higher than those from the five producing areas in Southeast Asia. Compared with other agarwood-inducing method, the sensory characteristics of the agarwood produced by agar-wit were relatively stable, and the average contents of the extract and total chromone were significantly higher, but the average content of agarotetrol was slightly lower than that of the agarwood produced by peripheral-inducing and iron-inducing. CONCLUSION: In terms of quality, the agar-wit is superior to the traditional agarwood-inducing method. It is not only applicable to the agarwood production of Aquilaria sinensis in China, but also applicable to the species of Aquilaria Lam. in Southeast Asian countries. The quality of agarwood produced by agar-wit is good in general, but the quality of the agarwood produced by A. sinensis is better than the species of Aquilaria Lam. in the Southeast Asian.
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Objective:To investigate overall quality status of rose bengal agar culture medium for drug control in China to provide technical support for the drug standard revision. Methods:Four quality control microorganisms, namely Aspergillus niger, Candida al-bicans, Staphylococcus aureus and Escherichia coli, were used to evaluate the quality of rose bengal agar culture medium from 7 domes-tic and foreign manufacturers. Results:Including the control medium, the pH value of all the 8 media was different. The growth rate of Aspergillus niger and Candida albicans in the seven tested media were all above 0. 7 without significant difference (P>0. 05). The selectivity factors of Staphylococcus aureus in the seven media and the control medium was more than 6. The eight media showed differ-ences in the selectivity factors of Escherichia coli. Among them, A, B, C, E, G and the control medium had no inhibitory ( selectivi-ty) , while D and F showed certain selectivity. Conclusion:The rose bengal agar media from different manufacturers for drug control show similar growth-promoting ability with different pH value and selectivity to Escherichia coli.
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OBJECTIVE:To compare the quality of fungus and bacteria counting culture medium of drug microbial limit test among different manufacturers.METHODS:Culture medium for 7 kinds of nutrient agars and 5 kinds of sodium rose red bengal agars for sale or self-made were enrolled in study.The content of water and nitrogen,pH value were determined.The number of microcolonies which were recovered from 5 kinds of representative bacterial strains was calculated,and the size and number of microcolonies were compared.RESULTS:Physicochemical index of culture medium,the size and number of microcolonies were different among different manufacturers.CONCLUSION:The quality of culture medium is different among different manufacturers,which is associated with physicochemical index.
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To compare the quality of cultivated species with w ild species of Desmodium styracifolium (Osbeck) Merr. TLC and UV were used to study their qualities. Results showed that there was no obvious di fference between them.