RESUMO
Objective To observe the therapeutic efficacy of 6% hydroxyethyl starch 130/0.4 (trade name: voluven) and polygeline on dog with hemorrhagic shock and their effect on immune function of red blood cells (RBC). Methods Eighteen dogs were randomized into three groups (n=6 in each group): normal sodium group, voluven group and polygeline group. Hemorrhagic shock models were set up according to Wiggers’ method. The mean arterial pressure value was bled to (45.56?3.69) mmHg within 10 min and maintained at this level for 60 min. Subsequently the dogs were resuscitated with normal saline or voluven or polygeline. The hemodynamics were measured before and 60 min after shock and 10, 30 and 60 min after infusion. The concentrations of MDA in plasm, C3b receptor garland rate (RBC-C3bRR) and RBC immune complexes garland rate (RBC-ICR) were measured. Results At 60 min after shock, MAP and CO were significantly lower than these before shock (P
RESUMO
Objective: To study the effect of Bazhen decoction on red blood cell immunity in rats with Qi deficiency.Methods:After the rats were forced to swim for two weeks continuously, the rat model of Qi deficiency was established. Bazhen group and normal group were fed with Bazhen decoction (20g?kg -1 ,daily), and distilled water for fourteen days, respectively. The changes in red blood cell c 3b receptor rosette rate (RBC.c 3bRR) and red blood cell immune complexes rosette rate (RBC.ICRR) were studied while the influences of Bazhen decoction on above mentioned parameters were observed. Results: In Qi deficiency syndrome the levels of RBC.c 3bRR were markedly depressed, while RBC.ICRR markedly elevated. After feeding Bazhen decoction orally to rats model with Qi deficiency syndrome, the levels of RBC. c 3bRR were elevated, while RBC.ICRR depressed (compared with Qi deficiency model group, P
RESUMO
Siegel et al (1981) first put forward a new concept of the erythrocyte immune system. Guo Feng et al (1982) were the first ones to find red blood cell could adhering to complement coated yeast cells, red blood cell could adhering to complement coated cancer cells in 1986, blood cells (including Red blood cell, white blood cell) could adhering to complement Coated Cancer cells in 1999, and erythrocytes accounted for 99% in the formation cosettes when adherent to cancer cells. In 2001, The author and his colleagues (2001) found that activity of red cell in enhancing lymphocyte CR1 innate immune activity (or T lymphocyte or NK cell activity ) might be related to ECR1 genomic density polymorphism. In 2005the author postulated the main pathway of erythrocyte innate immune reaction, and the proposed algorithm consisted of: activation of complements by antigen (cancer cells or yeast cells et al) adherence of C3b adherence to red blood cell adheherence to white blood cells activation of blood immune reaction system. The author hypothesized that in blood immune reaction against antigen, there were 4 essential elements: antigen, plasma (complement), red blood cells, and white blood cells. Red blood cells and complement might play an important role in regulation of blood immune reaction. We attempted to establisha new experimental system of modern immunology. As cancer cell (or yeast cells) is a kind of antigenic cell, which can activate immunoreaction of the blood, a new experimental system of hematogenic immunoreaction algorithm could be built. Changes in various immunity indexes, including blood cell adherence rate, Il-8, IL-6, IL-12, CD35, CD44, CD55, CD59, CD4, CD8, CD2, CD25,CXCR4, Fy6, ete, indicate that cancer cells (S180) can activate immunoreaction, of the blood. A new experimental systerm enables us to study the immanent relationship between whole blood cells and plasma, red blood cell and white blood cells in immunoreaction, and it also provides us a useful method to study innate and adaptative immunity, hematogenic immunoreaction road map, and clinical immunotherapy. It is a new system for experimental study of modern systemic immunology.