RESUMO
Objective: To compare the influences of Shenfu Qiangxin Pillsat different medication time and dosage on corpulmonale model rats, and define the best drug use entry point of Shenfu Qiangxin Pills in rats with corpulmonale. Methods: The smudging combined with ip rattlebush alkaline were used to prepare rats corpulmonale model, and the effects of Shenfu Qiangxin Pills at different drug administration time and dosage on right ventricle hypertrophy index, alveolar mean linear intercept, alveolar numbers, airway inflammation pathological scores, and matrix metalloproteinase-9 (MMP-9) in rats were investigated. Results: Comparing with the model group, the right ventricle hypertrophy index of rats in high- and low-dosage groups of Shenfu Qiangxin Pills was significantly reduced. Alveolar number was significantly increased. Serum ANP level was reduced. Cardiopulmonary MMP-9 level was reduced (P < 0.05). The effects in the high-dosage group was significant. Conclusion: The short-term drug administration of Shenfu Qiangxin Pills for two weeks was superior to long-term precaution drug administration for four weeks by reducing right ventricle hypertrophy index and inhibiting airway reaction, and existed dose-effect relationship.
RESUMO
Objective To investigate whether the effect of puerarin on right ventricle hypertrophy of pulmonary hypertensive rats induced by chronic hypoxia-hypercapnia was related to new peptide Apelin or its receptor(APJ).Methods Thirty male Sprague-Dawley rats were randomly divided into three groups, they are control group,hypoxia-hypercapnia 4-week model group,and hypoxia-hypercapnia 4-week plus puerarin group.The concentrations of Apelin-36 protein in plasma and homogenate of right ventricular muscle were detected by radioimmunoassay.The mRNA expressions of Apelin and APJ in right ventricu- lar muscle were measured by semi-quantitive reverse transcription polymerase chain reaction(RT-PCR). Results The weight ratio of right ventricle to left ventricle plus septum[RV/(LV+S)] in model group was significantly higher than that in control group(P0.05).The plasma concen- tration of Apelin-36 protein in model group was significantly higher than that in control group(P