RESUMO
OBJECTIVE:To examine the effects of sanazole and of sanazole combining with ?-ray irradiation on the colony formation ratio of granuloid/macrophage committed progenitor cell(CFU-GM)from mouse bone marrow in vivo.METHODS:(1)After a series of doses of sanazole solution(50,100,200mg/kg)was given to mice intravenously,CFU-GMs were separated from mouse bone marrow for evaluating their colony formation ratio.(2)To evaluate the radiosensitization of sanazole,a series of doses of sanazole solution(50,100,200mg/kg)was given to mice intravenously 30min before the mice were exposed to ?-ray irradiation in different doses(5,10,20Gy)respectively.After each treatment,CFU-GMs were separated from mice marrow for counting the ratio of colony formation.RESULTS:(1)Sanazole especially in large doses didn’t showed considerable cytotoxicity to CFU-GM colony formation in mice in vivo.(2)The inhibiting effect of a series of doses of sanazole solution combined with a series of doses of radiation (5,10,20Gy)on colony formation of CFU-GM from mice bone marrow was the same as that of radiation alone.CONCLUSION:Large doses of sanazole showed no obvious effect on the colony formation of CFU-GM of mouse bone marrow in vivo.No radiosensitization to the colony formation of CFU-GM was found in vivo when a series of doses of sanazole in combination with radiation were used.