RESUMO
Objective To observe the tissular pathological and ultrastructural changes of gastric mucosa with experimental atrophic gastritis caused by salt-water in rats and to explore the relationship between atrophic gastritis and high-salt diet.Methods The atrophic gastritis rat model was made by salt-water perfusion(0.15g/ml,2.5ml/d).The tissue of sinus ventriculi was detected with histopathologic examination and the ultrastructure of gastric mucosa was observed by scanning electron microscope.Results Under light microscope,the gastric gland was shrunk obviously in high-salt water perfusion group after high-salt water was given for 24 weeks.The muscle in muscular layer was hyperplasia and cramped into the lamina propria.The glandular epithelium in the upper 1/3～2/3 edge of the gland was atrophic.The gastric micro-depression became broaden and the width of cervical part of gastric pit became narrow.Under the scanning electromicroscope,the gastric mucosa was comparted into many gastric areas in the control group.Round or elliptic epithelium cells lined on the wall of gastric pits were in the same size and had short and spare microvillus.The epithelium was lined regularly and covered by laminar mucus.In salt-water perfusion group after the salt-water was given for 24 weeks,the surface of the gastric mucosa became thin and flat,and the glandular cells became rough.The mucosa between lumens of gland was broadened and the limited exfoliation of mucosa also could be seen.When the salt-water was given for 32 weeks,the gastric epithelial cells were shrunk,and the diameter of lumen of gland was broadened.There was breakage in the cell surface.Erosions with various size and shape and fibrous exudation could be seen.Conclusion Salt-water perfusion may cause the damage of the epithelia of gastric mucosa in experimental rats and long-term high-salt stimulation can induce the injury and atrophy of gastric mucosa.
RESUMO
Using scanning electromicroscope, seven species of anopheline mosquito eggs were studied. Among them, the microstructure of exochorion of Anopheles liangshanensis. An. kweiyangensis An. kunmingensis, An. hyrcanus and An. messeae were not reported before. The results showed that ultrastructure of plastron network, frill and tubercles of deck were useful in distinguishing sibling species of anopheline eggs. Microstructure of float had little difference between species. The micropylar area and lobed tubercles presented obvious intraspecific variations, so, it should be careful for using in classification.