Protection Effect of Schisanhenol on Oxidative Damage Induced by H2O2 in SH-SY5Y Cells / 中国药学杂志

OBJECTIVE: To investigate the protective effect and possible mechanism of schisanhenol(Sal) in SH-SY5Y cell induced by H2O2. METHODS: SH-SY5Y cells were treated with Sal (1, 10 and 50 μmol•L-1) for 4 h and then exposed to H2O2 100 μmol•L-1 for 24 h. Cell viability was measured by MTT assay. The expressions of silent information regulator 1(SIRT1), PGC-1α and p-tau (S396) protein were detected using Western blotting. RESULTS: MTT results showed that Sal significantly increased the survival rate of SH-SY5Y cell damaged by H2O2. Western blotting analysis showed that H2O2 reduced the expressions of SIRT1 and PGC-1α in SH-SY5Y cells. However, tau protein content was increased by H2O2 at p-tau(S396) sites. Sal treatment significantly increased the levels of SIRT1 and PGC-1α and decreased p-tau(S396) level induced by H2O2 in SH-SY5Y cells. CONCLUSION: These results suggest that Sal has a protective effect on H2O2 damaged SH-SY5Y cells, which is related to up regulating the expressions of SIRT1 and PGC-1α protein and decreasing the phosphorylation of tau protein.

Protection effect of schisanhenol on learning and memory acquired disorder induced by scopolamine in mice / 中国药学杂志

OBJECTIVE: To study the effect and mechanism of schisanhenol on learning and memory acquired disorder induced by scopolamine.

Pharmacokinetic study of schisanhenol via femoral intravenous administration in rats / 中草药

Objective To establish an HPLC method for the determination of schisanhenol in plasma and to study the pharmacokinetics of schisanhenol in rats.Methods After sedimentation by methanol,plasma samples were then prepared based on a liquid-liquid extraction by ether.The extracted samples were analyzed by liquid chromatography.Schisanhenol was eluted on Eclipse XDB-C18 Agilent(250 mm?4.6 mm,5 ?m) column,using a mobile phase of acetonitrile-H2O(65∶35),and detected at 254 nm.The plasma concentration of schisanhenol in rats was determined after iv administration of 18 mg/kg,and the data were processed with the pharmacokinetic software 3P87.Results Calibration curves were linear over 0.1—2.5 ?g/mL (r2=0.999) and the LOD was 10 ng/mL.The recoveries of schisanhenol from plasma were between 88%—110%,and the RSD values of intra-day and interday assay were below 15%.After iv administration at 18 mg/kg,the schisanhenol concentration-time curve confirmed in a two-compartment model and the pharmacokinetic parameters of t1/2?,t1/2?,V,AUC,MRT were(0.22?0.11) h,(1.19?0.22) h,(12.81?2.91) L/kg,(1.32?0.19) ?g/mL/h,(1.51?0.24) h,respectively.Conclusion A reliable HPLC-DAD method is developed for the determination of schisanhenol in rat plasma and it is applicable to the in vivo analysis.