RESUMO
Objective To understand correctly the hook like effect of the immune transmission and transmission turbidity in the automatic biochemical analyzer and the prevention of the hook like effect .Methods First ,it had the function set of measurement linear range of the upper limit of the instrument and the instrument automatic dilution redo ,and then ,had determination of excee‐ding the upper limit of measurement range and artificial dilution before the sample setting .Results The group average value was 2 .49 g/Lbefore the determination setting ,3 .31 g/L after the determination setting ,and 3 .33 g/L artificial dilution determination . Conclusion High dose hook effect makes the strong positive to weak positive samples of measurement by error ,even false negative results ,and high concentration sample makes low value .So the set of samples and dilution to determination of instrument ,measured values are more close to the true value .