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Objective To investigate the role of interferon ?on smooth muscle cells proliferation and migration after balloon injury. Methods Animal model of rabbit iliac artery balloon injury was set up, smooth muscle cells derived from injured artery were cultured. Smooth muscle cells were divided into four groups ( control, TGF ? 1, IFN ?, TGF ? 1 and IFN ?).Cells from each group were treated with medium or TGF ? 1 (10 ng/ml) and/or IFN ?(500 u/ml) for 72 h separately. Smooth muscle cell proliferation was determined by cell count and MTT, migration of smooth muscle cells was also detected. Matrix metalloproteinase 2 was also detected by zymography. Results Our results showed that, compared with group control(2.875?0.323?10 5 cells/ml,279.9?8.129 ?m), TGF ? 1 increased cell count (4.188?0.239?10 5 cells/ml, P
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Objective To investigate the influence of endogenous transforming growth factor ? 1 (TGF? 1) on the cell cycle regulation and proliferation of bladder cancer. Methods A constructed replication defective retroviral vector pRevT? AS,which carried antisense RNA of TGF? 1,was transfected to a bladder cancer cell line EJ.The proliferation and clone formation of transferred cells were observed in vitro,and the alteration of cell cycle was also detected by flow cytometric analysis. Results TGF? 1 antisense RNA was transferred into EJ cell and expressed efficiently.After the inhibition of target gene expression in EJ cells,the reduced growth and clone formation rates were demonstrated,and the proliferative indexes were decreased by 12%.The ratios of G 0 and G 1 stage cells to the antisense RNA transfected EJ cells were increased,simultaneously,the ratio of S stage cells to the antisense RNA transfected EJ cells ratios were decreased,compared with the control group. Conclusions The proliferative mechanism of endogenous TGF? 1 in bladder cancer cells is to stimulate the G 1 to S stage transition.
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Objective To investigate the influence of transforming growth factor ? 1 to expression of tumor necross factor ?(TNF ?) after cerebral ischemic reperfusion injury.Methods Using thread embolism method to develop the model of focal cerebral ischemic reperfusive injury in rats, different dose of TGF ? 1 or 0 9% NaCl was injected intracerebroventricularly.Neurological functional dificit scales in the different dose of TGF ? 1 groups and the expression of TNF ? were observed Results In both big and small dose of TGF ? 1 groups,the expression of TNF ? decreased after cerebral ischemic reperfusion,and the neurological functional dificit scales were significantly lower than those of the control.There was no significant difference between the group of big and small dose of TGF ? 1 Conclusion TGF ? 1 may have a protective effect on cerebral ischemic reperfusive injury by inhibiting expression of TNF ?,and it may be irrelative with the dose
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Objective To investigate the influence of the aberrant protein expression and mRNA transcription of transforming growth factor ? 1(TGF? 1) on the biological behavior of human bladder transitional cell carcinomas. Methods The expression of TGF? 1 was investigated in 74 specimens of TCCs by SP immunohistochemistry staining, and the level of TGF? 1 mRNA were determined in 43 cases of TCCs by the method of quantitative RT PCR. Results The positives expression rate of TGF? 1 was 89.2%. Superficial tumors had lower overexpressive rate of TGF? 1(33.3%) than the invasive TCCs(83.8%), P
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Objective To investigate the protective effect of Quercetin on kidneys in diabetic rats. Methods STZ induced diabetic rats were given quercetin 100 mg?kg -1 ?d -1 for 8 weeks. Urinary albumin excretion rate (UAER) was measured by radioimmunoassay. The changes of creatinine clearance rate (Ccr) and glomerular protein kinase C (PKC) activities were determined. The expression of TGF ? 1 mRNA of renal cortex in diabetic rats were determined by RT PCR analysis. The glomerular changes were also observed morphologically. Results In untreated diabetic rats, Ccr, UAER, kidney weight/body weight and PKC activity in renal glomeruli were significantly increased, the expression of TGF ? 1 mRNA in renal cortex was elevated, and glomerular hypertrophy existed. After Quercetin treatment, Ccr, UAER, PKC activity and the expression of TGF ? 1 mRNA were markedly reduced as compared with those of untreated diabetic rats in 2 and 8 weeks, no significantly abnormal changes in kidney morphology were observed in Quercetin treated group. Conclusion Quercetin ameliorates early diabetic renal hyperdynamic abnormality via inhibiting PKC activity, in which inhibiting of TGF ? 1 production seems to be involved. Reduction of the PKC activity is important in preventing or delaying the development of diabetic nephropathy.
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Objective To analyze the actions of transforming growth factor ? 1 (TGF-? 1) and tumor necrosis factor ?(TNF-?) in the liver fibrosis formation in chronic viral hepatitis patients and liver cirrhosis patients.Methods Serum levels of TGF-? 1 and TNF-? were measured by ELISA in 107 patients with chronic liver diseases,liver biopsy was performed in 23 patients in order to identify pathological stages under optic microscope.Results The serum levels of TGF-? 1(?g/L) in mild,middle and severe groups of chronic viral hepatitis and liver cirrhosis group were 14 2?5 9,20 1?7 0,30 2?6 7 and 32 6?7 5 respectively.The serum levels of TNF-? (pg/L) were 6 1?3 2,29 8?18 6,57 3?22 5 and 96 7?38 2 respectively.The serum levels of above two markers increased with hepatitis progression.The results of liver biopsy revealed that the changes in these two markers were related to liver cirrhosis degree.Conclusions TGF-? 1 and TNF-? are related to liver cirrhosis formation.
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Objective To study the regulatory effect of TGF ? 1 on the expression of multiple genes in prostatic stromal cells in vitro. Methods The primary culture of prostatic stromal cells (including fibroblasts and smooth muscle cells ) have been established and cultured to 4～6 passages. Then the cells were cultured in the medium with various concentration of TGF ? 1(0.01, 0.10, 1.00 and 10.0 ng/ml)for 48h. By semi quantitative RT PCR method, the androgen receptor(AR), TGF ? 1, bFGF and smoothlin mRNA were measured. Results The expression of AR could be stimulated by low concentration of TGF ? 1 ( P
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Objective To investigate the influence of vitamin E on type Ⅰ collagen, messenger RNA expression of transforming growth factor ? 1 (TGF? 1) and tissue inhibitor of matrix metalloproteinases-2 (TIMP 2) in hepatic stellate cells (HSC). Methods HSC s were cultured in vitro, the effect of vitamin E on type Ⅰ collagen was observed by mean of immunohistochemistry; mRNA expression of TGF? 1 and TIMP 2 were investigated with in situ hybridization. Results In experiment group, vitamin E markedly inhibited expression of type Ⅰ collagen of HSC as compared with control group, but could not suppress mRNA expression of TGF? 1 and TIMP 2.Conclusions Anti-fibrotic effect of Vitamin E may be implemented through inhibiting the expression of type Ⅰ collagen.
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0.05 ),the levels of CSF TGF ? 1 were higher significantly than that of plasma levels and CSF in the controls( P
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Objective To observe the changes of the levels of transforming growth factor ? 1(TGF ? 1),fibronectin(FN),laminin(LN) and collagen content and myocardium ultrastructure in streptozotocin experimental diabetes mellitus rats.To study the role of TGF ? 1 and extracellar matrix(ECM) in diabetic cardiomyopathy episode.Methods Observing myocardium ultrastructure by electron microscope and assaying TGF ? 1,FN,LN by immunohistochemistric,and observing collagen content by VG staining.Results TGF ? 1,FN,LN were expressed more in STZ inroduced diabetic rats than in normal rats.The difference between two groups was significant ( P
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Objective To investigate the mechanism of Quercetin treatment on diabetic nephropathy.Methods Quercetin 100mg/(kg?d) was given to diabetic rats.Urinary albumin excretion was measured by radioimmunoassay.The expression of TGF ? 1 protein and TGF ? 1 mRNA in renal cortex of diabetic rats were determined by immunohistochemical staining and RT PCR analysis.Results Quercetin treatment decreased urinary albumin excretion in diabetic rats.TGF ? 1 immunohistochemical staining and TGF ? 1 mRNA levels in Quercetion treated group was significantly decreased than untreated DM group ( P
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Objective:To study the correlation between plasma TGF? 1 and other liver fibrosis parameters.Methods: Plasma TGF? 1 was detected with ELISA in 71 patients with chronic liver diseases;serum hyalauronic acid (HA), laminin (LN),type Ⅲ procollagen (PCⅢ),type Ⅳ collagen(CoⅣ) were detected with RIA. Results:Plasma TGF? 1 levels were elevated in chronic liver disease patients than those in normal control( P