The Correlation between Vitamin D Receptor Gene Polymophism and Bone Metabolism Disorder in Children with Nephrotic Syndrome / 昆明医科大学学报

Objective To study the correlation between VDR gene polymorphism and the transformation of bone markers in children with nephrotic syndrome and to observe whether active intervention treatment of vitamin D with nephrotic syndrome is influenced by VDR gene polymorphism. Methods We used polymerase chain reaction restriction fragment length polymorphism (PCR- RFLP) technology to detect the VDR genotypes of 70 children with nephrotic syndrome (one group received hormone for 2 weeks and the other received no hormone therapy) . Then we detected bone metabolism index among patients with nephrotic syndrome and 30 normal children (control group) and compared the index between the two groups with and without hormone therapy.We divided 70 children with nephrotic syndrome into the AA+Aa genotype group and the aa genotype group to study the difference between various genotypes of bone metabolism indexes. After retesting bone metabolism index of those children receiving the same dose of calcitriol therapy after 2 weeks, we divided them into three groups AA genotype, Aa genotype and aa genotype to detect the changes of bone metabolism index in different genotypes.Results (1) The calcitonin and serum phosphorus of children with AA+Aa genotype were significantly lower than those of childrenwith aa genotype (P＜ 0.05);25 (OH) D3 of children with aa genotype was significantly lower than that of ones with Aa + Aa genotype (P＜ 0.05) . (2) The changes of bone metabolism index in children with nephrotic syndrome after receiving the same dose of calcitriol for 2 weeks: the calcitonin and 25 (OH) D3 in AA+Aa genotype group were significantly higher after treatment (P＜0.05) .Conclusions Vitamin D receptor gene ApaⅠpolymorphism may be a genetic susceptibility factors affecting bone metabolic abnormalities. AA and Aa genotype may be a protective factor of bone metabolic abnormalities.The AA+Aa genetype of Vitamin D receptor gene ApaⅠpolymorphism response to treatment of calcitriol is positive.

Association of Calcium Intake and Bone Mineral Density by Vitamin D Receptor Genotype among Elderly Women Living in Rural a Area / 대한지역사회영양학회지

Recent studies have shown that environmental, biochemical, nutritional or genetic factors affect bone mineral density (BMD). The purpose of the present study was to investigate the effect of vitamin D receptor (VDR) gentotype and nutritional status on BMD of elderly women living in a rural area. Three hundred thirty five elderly women over 65 years in Asan participated the study. Data for demographic and nutrient intakes were obtained by survey with a two day 24 hr recall method. BMD was measured by broadband ultrasound attenuation (BUA) using quantitative ultrasound (QUS). VDR genotypes of the subjects analyzed with Bsm I restriction enzyme were bb (92%), Bb (7%) and BB (1%). No differences were found between genotypes bb and Bb/BB in age, menopausal age, body mass index and body fat. BUA of bb genotype was higher (62.5 +/- 15.6 dB/MHz) than Bb/BB genotype (56.1 +/- 17.6 dB/MHz) by Student's t-tests. Correlation analyses showed strong negative correlation of BMD and age, but positive correlation with BMI, energy and calcium intake. When subgroup analyses were conducted after stratification by the median calcium intake level (412.9 mg/d), the above median calcium intake group showed significant difference in BUA by VDR genotype while the lower median calcium intake group did not show significant difference. The current study confirmed interaction of calcium intake and VDR genotype in association with BMD. Further nutritional intervention will be needed to improve calcium status of the elderly women living in rural areas.

Study of the relationship between gene polymorphism of vitamin D receptor, calcitonin receptor and bone mineral density of the Han nationality woman in Hebei / 中国康复理论与实践

@#ObjectiveTo study the relationship between vitamin D receptor (VDR) gene polymorphism and calcitonin receptor (CTR) gene polymorphism, and bone mineral density (BMD) of the Han nationality woman in Hebei, explore the pathogenesis of osteoporosis (OP) at the gene level.MethodsPolymorphisms of VDR gene and CTR gene were analyzed by restriction fragment length polymorphisms (RFLPs) in 60 Han nationality women in Hebei.ResultsBb genetype of VDR had lower BMD values at all sites which were measured compared with bb genetype (P<0.05); CC genetype of CTR had tendency for lower BMD values at the L1～L4 compared with CT genetype (0.05<P<0.1); BMD value of CCBb genetype was the lowest.ConclusionBb genetype of VDR has a relationship with lower BMD. CCBb genetype can act as a heredity mark of OP in Chinese Han nationality woman.