Effect of morphine on the Kv channel in bronchial smooth muscle in guinea-pigs / 中国药理学通报

Aim To explore the effect of the morphine(MP) on the Kv channel in bronchial smooth muscle in guinea-pigs (GPBSM) and expressions of the Kv 1.5 mRNA and protein . Methods The effect of the MP on the Kv channel was studied by patch clamp recording in GPBSM and expressions of the Kv 1.5 mRNA and protein were measured by Western-blot and RT-PCR techniques. Results Morphine remarkably restrained the Kv channel current and the expression of the Kv 1.5 mRNA and protein in cultured GPBSM (n=6, each group, P